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Sphingomyelinases (SMase) are enzymes that catalyze the hydrolysis of sphingomyelin into ceramide and phosphorylcholine. Ceramide appears to be a second messenger lipid in programmed apoptosis, cell differentiation, and cell proliferation. Sphingomyelinase is probably a major source of ceramide in cells. Signal transduction mediated by ceramide leads cells to produce cytokine induced apoptosis during several inflammatory responses. SMase are also relevant toxins in several microorganisms. The main objective of this research is to identify SMase activity of<jats:italic>T. vaginalis<\/jats:italic>in the total extract (TE), P30, and S30 subfractions from brooked trophozoites. It was found that these fractions of<jats:italic>T. vaginalis<\/jats:italic>have SMase activity, which comes principally from P30 subfraction and was mainly type C. Enzymatic activity of SMase increased linearly with time and is pH dependent with two peaks by pH 5.5 and pH 7.5. The addition of manganese to the reaction mixture increased the SMase activity by 1.97.<\/jats:p>","DOI":"10.1155\/2013\/679365","type":"journal-article","created":{"date-parts":[[2013,8,19]],"date-time":"2013-08-19T21:01:53Z","timestamp":1376946113000},"page":"1-8","source":"Crossref","is-referenced-by-count":3,"title":["Sphingomyelinase Activity of<i>Trichomonas vaginalis<\/i>Extract and Subfractions"],"prefix":"10.1155","volume":"2013","author":[{"given":"Francisco","family":"Gonz\u00e1lez-Salazar","sequence":"first","affiliation":[{"name":"Divisi\u00f3n de Biolog\u00eda Celular y Molecular, Centro de Investigaci\u00f3n Biom\u00e9dica del Noreste, Instituto Mexicano del Seguro Social, Administraci\u00f3n de Correo No. 4, 2 de abril 501 Colonia Independencia, 64720 Monterrey, NL, Mexico"},{"name":"Departamento de Ciencias B\u00e1sicas, Divisi\u00f3n de Ciencias de la Salud, Universidad de Monterrey, Avenida Morones Prieto 4500 Pte, 66238 San Pedro Garza Garc\u00eda, NL, 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