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To clarify this mechanism, we constructed the wild and mutated<jats:italic>Wisp3<\/jats:italic>expression vectors and transfected into human chondrocytes lines C-20\/A4;<jats:italic>Wisp3<\/jats:italic>proteins subcellular localization, cell proliferation, cell apoptosis, and<jats:italic>Wisp3<\/jats:italic>-mediated gene expression were determined, and dynamic secretion of collagen in transfected chondrocytes was analyzed by<jats:sup>14<\/jats:sup>C-proline incorporation experiment. Mutated<jats:italic>Wisp3<\/jats:italic>protein increased proliferation activity, decreased apoptosis of C-20\/A4 cells, and aggregated abnormally in cytoplasm. Expression of collagen II was also downregulated in C-20\/A4 cells transfected with mutated<jats:italic>Wisp3<\/jats:italic>. Wild type<jats:italic>Wisp3<\/jats:italic>transfection increased intracellular collagen content and extracellular collagen secretion, but the mutated<jats:italic>Wisp3<\/jats:italic>lost this function, and the peak phase of collagen secretion was delayed in mutated<jats:italic>Wisp3<\/jats:italic>transfected cells. Thus abnormal protein distribution, cell proliferation, collagen synthesis, and secretion in<jats:italic>Wisp3<\/jats:italic>mutated chondrocytes might contribute to the pathogenesis of SEDT-PA.<\/jats:p>","DOI":"10.1155\/2013\/679763","type":"journal-article","created":{"date-parts":[[2013,3,19]],"date-time":"2013-03-19T17:01:16Z","timestamp":1363712476000},"page":"1-9","source":"Crossref","is-referenced-by-count":13,"title":["Dysfunction of Collagen Synthesis and Secretion in Chondrocytes Induced by<i>Wisp3<\/i>Mutation"],"prefix":"10.1155","volume":"2013","author":[{"given":"Min","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Endocrinology and Metabolism, The Second Xiang-Ya Hospital of Central South University, Changsha, Hunan 410011, China"},{"name":"Department of Endocrinology and Metabolism, Xiang-Ya Hospital of Central South University, Changsha, Hunan 410008, 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