{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T14:28:09Z","timestamp":1781706489318,"version":"3.54.5"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical and Developmental Immunology"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>CD8+ T cells have been reported to play an important role in defense against<jats:italic>B. abortus<\/jats:italic>infection in mouse models. In the present report, we use CD8 knockout mice to further elucidate the role of these cells in protection from<jats:italic>B. melitensis<\/jats:italic>infection. Mice were immunized orally by administration of<jats:italic>B. melitensis<\/jats:italic>WR201, a purine auxotrophic attenuated vaccine strain, then challenged intranasally with<jats:italic>B. melitensis<\/jats:italic>16M. In some experiments, persistence of WR201 in the spleens of CD8 knockout mice was slightly longer than that in the spleens of normal mice. However, development of anti-LPS serum antibody, antigen-induced production of<jats:italic>\u03b3<\/jats:italic>-interferon (IFN-<jats:italic>\u03b3<\/jats:italic>) by immune splenic lymphocytes, protection against intranasal challenge, and recovery of nonimmunized animals from intranasal challenge were similar between normal and knockout animals. Further, primary<jats:italic>Brucella<\/jats:italic>infection was not exacerbated in perforin knockout and Fas-deficient mice and these animals\u2019 anti-<jats:italic>Brucella<\/jats:italic>immune responses were indistinguishable from those of normal mice. These results indicate that CD8+ T cells do not play an essential role as either cytotoxic cells or IFN-<jats:italic>\u03b3<\/jats:italic>producers, yet they do participate in a specific immune response to immunization and challenge in this murine model of<jats:italic>B. melitensis<\/jats:italic>infection.<\/jats:p>","DOI":"10.1155\/2013\/686919","type":"journal-article","created":{"date-parts":[[2013,10,28]],"date-time":"2013-10-28T21:04:48Z","timestamp":1382994288000},"page":"1-7","source":"Crossref","is-referenced-by-count":14,"title":["CD8 Knockout Mice Are Protected from Challenge by Vaccination with WR201, a Live Attenuated Mutant of<i>Brucella melitensis<\/i>"],"prefix":"10.1155","volume":"2013","author":[{"given":"Samuel L.","family":"Yingst","sequence":"first","affiliation":[{"name":"Department of Bacterial Diseases, Walter Reed Army Institute of Research, Silver Spring, MD 20910-5100, USA"},{"name":"Armed Forces Research Institute of Medical Sciences, 315\/6 Rajvithi, Bangkok 10400, Thailand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3708-2495","authenticated-orcid":true,"given":"Mina","family":"Izadjoo","sequence":"additional","affiliation":[{"name":"Diagnostics and Translational Research Center, Henry M. 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