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This study was designed to examine the protective effects and mechanisms of Nec-1 in concanavalin A- (ConA-) induced hepatitis in mice.\n                    <jats:italic>Methods.<\/jats:italic>\n                    C57BL\/6 mice were exposed to ConA via tail vein injection and injected intraperitoneally with Nec-1 or vehicle. Levels of serum liver enzymes and histopathology were determined. Levels of inflammatory cytokines with ConA-induced hepatitis were determined with real-time polymerase chain reaction (real-time PCR). The expression of TNF-\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , RIP1, and LC3 was detected with immunohistochemical staining. The expression of TNF-\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , IFN-\n                    <jats:italic>\u03b3<\/jats:italic>\n                    , IL2, IL6, caspase 3, RIP1, beclin-1, and LC3 protein was assessed by immunofluorescence and western blotting. Autophagosomes were observed with transmission electron microscopy (TEM).\n                    <jats:italic>Results.<\/jats:italic>\n                    Amelioration in liver functions and histopathological changes and the suppression of inflammatory cytokine production were observed in Nec-1-injected mice. Western blotting analysis showed that the expression of TNF-\n                    <jats:italic>\u03b1<\/jats:italic>\n                    , IFN-\n                    <jats:italic>\u03b3<\/jats:italic>\n                    , IL2, IL6, and RIP1 was significantly reduced in the Nec-1-injected mice, which was confirmed by immunofluorescence and immunohistochemistry. Autophagosome formation was significantly reduced by Nec-1 treatment, as the expression of beclin-1 and LC3, determined with immunofluorescence and western blotting.\n                    <jats:italic>Conclusion.<\/jats:italic>\n                    These results demonstrate that Nec-1 prevents ConA-induced liver injury via RIP1-related and autophagy-related pathways.\n                  <\/jats:p>","DOI":"10.1155\/2013\/706156","type":"journal-article","created":{"date-parts":[[2013,10,1]],"date-time":"2013-10-01T17:01:38Z","timestamp":1380646898000},"page":"1-15","source":"Crossref","is-referenced-by-count":57,"title":["Protective Effects of Necrostatin-1 against Concanavalin A-Induced Acute Hepatic Injury in Mice"],"prefix":"10.1155","volume":"2013","author":[{"given":"Yingqun","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, The Tenth People's Hospital of Tongji University, Shanghai 200072, 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