{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T09:50:22Z","timestamp":1778579422584,"version":"3.51.4"},"reference-count":17,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>A novel bacterium capable of growth on trichloroethylene as the sole carbon source was identified as<jats:italic>Stenotrophomonas maltophilia<\/jats:italic>PM102 by 16S rDNA sequencing (accession number of NCBI GenBank: JQ797560). In this paper, we report the growth pattern, TCE degradation, and total proteome of this bacterium in presence of various other carbon sources: toluene, phenol, glucose, chloroform, and benzene. TCE degradation was comparatively enhanced in presence of benzene. Densitometric analysis of the intracellular protein profile revealed four proteins of 78.6, 35.14, 26.2, and 20.47\u2009kDa while the extracellular protein profile revealed two distinct bands at 14\u2009kDa and 11\u2009kDa that were induced by TCE, benzene, toluene, and chloroform but absent in the glucose lane. A rabbit was immunised with the total protein extracted from the bacteria grown in 0.2% TCE + 0.2% peptone. Antibody preadsorbed on proteins from peptone grown PM102 cells reacted with a single protein of 35.14\u2009kDa (analysed by MALDI-TOF-mass-spectrometry) from TCE, benzene, toluene, or chloroform grown cells. No reaction was seen for proteins of PM102 grown with glucose. The PM102 strain was immobilised in calcium alginate beads, and TCE degradation by immobilised cells was almost double of that by free cells. The beads could be reused 8 times.<\/jats:p>","DOI":"10.1155\/2013\/714232","type":"journal-article","created":{"date-parts":[[2013,6,26]],"date-time":"2013-06-26T21:02:06Z","timestamp":1372280526000},"page":"1-9","source":"Crossref","is-referenced-by-count":10,"title":["Persistent Organic Pollutants Induced Protein Expression and Immunocrossreactivity by<i>Stenotrophomonas maltophilia<\/i>PM102: A Prospective Bioremediating Candidate"],"prefix":"10.1155","volume":"2013","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3939-4310","authenticated-orcid":true,"given":"Piyali","family":"Mukherjee","sequence":"first","affiliation":[{"name":"Department of Biotechnology, University of Burdwan, Golapbag More, Burdwan, West Bengal 713104, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1583-5735","authenticated-orcid":true,"given":"Pranab","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Haldia Institute of Technology, Haldia, West Bengal 721657, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1093\/toxsci\/kfl055"},{"issue":"5","key":"2","doi-asserted-by":"crossref","first-page":"332","DOI":"10.1007\/s002040050394","volume":"71","year":"1997","journal-title":"Archives of Toxicology"},{"issue":"6","key":"5","first-page":"1289","volume":"104","year":"1996","journal-title":"Environmental Health Perspectives"},{"key":"6","year":"1996"},{"issue":"11","key":"8","doi-asserted-by":"crossref","first-page":"1057","DOI":"10.1002\/bit.260411108","volume":"41","year":"1993","journal-title":"Biotechnology and Bioengineering"},{"issue":"4","key":"9","doi-asserted-by":"crossref","first-page":"1523","DOI":"10.1128\/AEM.63.4.1523-1530.1997","volume":"63","year":"1997","journal-title":"Applied and Environmental Microbiology"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.02914-0"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.syapm.2005.05.011"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/BF00170570"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1111\/j.1472-765X.2006.01910.x"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.bej.2005.03.005"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.4236\/aim.2012.23034"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1038\/210945a0"},{"issue":"1-2","key":"17","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","year":"1976","journal-title":"Analytical Biochemistry"},{"key":"18","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1016\/j.ymeth.2004.08.015","volume":"35","year":"2005","journal-title":"Methods"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080029"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2008-9-4-r74"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/714232.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/714232.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/714232.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,29]],"date-time":"2020-07-29T05:20:14Z","timestamp":1596000014000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2013\/714232\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":17,"alternative-id":["714232","714232"],"URL":"https:\/\/doi.org\/10.1155\/2013\/714232","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013]]}}}