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After a quiescent period, 4 <jats:italic>rfb<\/jats:italic>N (serogroup O1) positive water samples that were culture negative were detected by multiplex single\u2010tube nested PCR (MSTNPCR); 2 of these were also <jats:italic>ctx<\/jats:italic>A (cholera toxin) positive. From May to June 2012, 30 <jats:italic>V. cholerae<\/jats:italic> O1 isolates were obtained by culturing samples. These isolates were analyzed for the presence of virulence genes by PCR, intergenic spacer region 16S\u201023S PCR (ISR\u2010PCR), and pulsed field gel electrophoresis (PFGE). The isolates were positive for the <jats:italic>rfb<\/jats:italic>N gene and negative for the assessed pathogenic genes and were classified into 2 groups by ISR and the same profile by PFGE. Close genetic similarity was observed between them (2012) and environmental strains from 2004 to 2005, indicating the permanence of endemic <jats:italic>V. cholerae<\/jats:italic> O1 in the region.<\/jats:p>","DOI":"10.1155\/2013\/746254","type":"journal-article","created":{"date-parts":[[2013,2,25]],"date-time":"2013-02-25T21:02:02Z","timestamp":1361826122000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Aquatic Environment as a Reservoir of <i>Vibrio cholerae<\/i> O1 in Hydrographic Basins of the State of Pernambuco, Brazil"],"prefix":"10.1155","volume":"2013","author":[{"given":"Carina Lucena","family":"Mendes-Marques","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir da Mota","family":"Silveira Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula Rocha","family":"da Costa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana de Lira","family":"Nunes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandoval Vieira da","family":"Silva Filho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"\u00c2ngela Cristina Torres de Ara\u00fajo","family":"Figueir\u00f4a","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ernesto","family":"Hofer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alzira Maria Paiva","family":"de Almeida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilma Cintra","family":"Leal","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,2,25]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2008.06.015"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2009.10.003"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02026-08"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652006000200002"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02763.x"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.274.5295.2025"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2007.12.008"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2007.11.018"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.11.4.385-388.1980"},{"key":"e_1_2_6_10_2","first-page":"97","article-title":"Medium of Rugai and lysine motility combined in one single tube for the presumptive identification of enterobacteriaceae","volume":"32","author":"Pessoa G. 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