{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,25]],"date-time":"2026-07-25T08:08:20Z","timestamp":1784966900654,"version":"3.55.0"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p><jats:italic>Gokshuradi Yog (GY)<\/jats:italic>is a polyherbal ayurvedic formulation used traditionally for several decades in India for the treatment of urolithiasis. The aim of the present study was to determine the underlying mechanism of<jats:italic>GY<\/jats:italic>action in the management of calcium oxalate urolithiasis. The effect of<jats:italic>Gokshuradi polyherbal aqueous extracts<\/jats:italic>(<jats:italic>GPAEs<\/jats:italic>) was studied on various biochemical parameters involved in calcium oxalate formation by employing<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>methods.<jats:italic>GPAE<\/jats:italic>exhibited significant antioxidant activity against 1, 1-diphenyl-2-picrylhydrazyl free radical and inhibited lipid peroxidation in the<jats:italic>in vitro<\/jats:italic>experiments. The rat model of urolithiasis induced by 0.75% ethylene glycol (EG) and 1% ammonium chloride (AC) in water caused polyuria, weight loss, impairment of renal function, and oxidative stress and decreased antioxidant enzyme activities in untreated control groups. However,<jats:italic>GPAE-<\/jats:italic>(25, 50, and 100\u2009mg\/kg) treated groups caused diuresis accompanied by a saluretic effect and revealed significant increase in antioxidant enzyme activities along with decreased oxalate synthesizing biochemical parameters at higher doses. This study revealed the antiurolithic effect of<jats:italic>GPAE<\/jats:italic>mediated possibly through inhibiting biochemical parameters involved in calcium oxalate formation, along with its diuretic and antioxidant effects, hence supporting its use in the treatment of calcium oxalate urolithiasis.<\/jats:p>","DOI":"10.1155\/2013\/763720","type":"journal-article","created":{"date-parts":[[2013,3,11]],"date-time":"2013-03-11T21:01:14Z","timestamp":1363035674000},"page":"1-9","source":"Crossref","is-referenced-by-count":17,"title":["Exploring Antiurolithic Effects of Gokshuradi Polyherbal Ayurvedic Formulation in Ethylene-Glycol-Induced Urolithic Rats"],"prefix":"10.1155","volume":"2013","author":[{"given":"Amol L.","family":"Shirfule","sequence":"first","affiliation":[{"name":"Food and Drug Toxicology Research Centre, National Institute of Nutrition, Hyderabad 500007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Venkatesh","family":"Racharla","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, CMR College of Pharmacy, Hyderabad 500007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. S. Y. H.","family":"Qadri","sequence":"additional","affiliation":[{"name":"Pathology Division, National Institute of Nutrition, Hyderabad 500007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arjun L.","family":"Khandare","sequence":"additional","affiliation":[{"name":"Food and Drug Toxicology Research Centre, National Institute of Nutrition, Hyderabad 500007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)66027-0"},{"key":"2","first-page":"720","volume-title":"Nephrolithiasis","year":"1992"},{"key":"3","year":"1992"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1080\/10496470902787493"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2003.04.002"},{"issue":"3","key":"6","first-page":"93","volume":"15","year":"1971","journal-title":"Indian Journal of Physiology and Pharmacology"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(03)00014-X"},{"key":"8","volume":"1","year":"1976"},{"key":"9","year":"1956"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(89)90072-X"},{"issue":"5","key":"11","first-page":"473","volume":"35","year":"1997","journal-title":"Indian Journal of Experimental Biology"},{"issue":"4","key":"12","first-page":"381","volume":"39","year":"2001","journal-title":"Indian Journal of Experimental Biology"},{"key":"13","first-page":"62","volume":"2","year":"2005","journal-title":"The African Journal of Traditional, Complementary and Alternative Medicines"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(95)01291-5"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2005.08.058"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2005.09.030"},{"key":"17","first-page":"110","volume-title":"Herbal drugs of Himalaya (medicinal plants of Garhwal and Kumaon regions of India)","volume":"15","year":"1998"},{"key":"18","first-page":"393","year":"1989"},{"issue":"2","key":"19","first-page":"14","volume":"5","year":"1983","journal-title":"Journal of the Chemical Society of Pakistan"},{"issue":"2","key":"20","first-page":"256","volume":"38","year":"2007","journal-title":"Southeast Asian Journal of Tropical Medicine and Public Health"},{"key":"21","first-page":"513","volume":"1","year":"2008","journal-title":"Pharmacologyonline"},{"key":"22","first-page":"179","volume":"45","year":"2004","journal-title":"Botanical Bulletin of Academia Sinica"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(03)00142-9"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(98)00194-9"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-410X.2003.04289.x"},{"key":"26","doi-asserted-by":"crossref","first-page":"263","DOI":"10.1093\/clinchem\/18.3.263","volume":"18","year":"1972","journal-title":"Clinical Chemistry"},{"issue":"2","key":"27","doi-asserted-by":"crossref","first-page":"214","DOI":"10.1093\/clinchem\/33.2.214","volume":"33","year":"1987","journal-title":"Clinical Chemistry"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(90)86141-H"},{"issue":"1","key":"29","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1016\/0304-4165(79)90289-7","volume":"582","year":"1979","journal-title":"Biochimica et Biophysica Acta"},{"key":"30","first-page":"273","volume-title":"Enzymes I: oxidoreductases, transferases","year":"1987"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(66)09069-4"},{"key":"32","volume":"87","year":"1965"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1007\/BF00307936"},{"key":"34","first-page":"707","volume":"5","year":"1991","journal-title":"Scanning Microscopy"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1159\/000083547"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(03)00764-7"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/S0955-2863(03)00033-0"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2005.05579.x"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000141311.51003.87"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)65141-3"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1007\/s00240-007-0122-4"},{"key":"42","first-page":"299","volume":"13","year":"1999","journal-title":"Scanning Microscopy"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2007.11.039"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2013\/763720.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2013\/763720.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2013\/763720.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,7,23]],"date-time":"2020-07-23T18:57:17Z","timestamp":1595530637000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/ecam\/2013\/763720\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":43,"alternative-id":["763720","763720"],"URL":"https:\/\/doi.org\/10.1155\/2013\/763720","relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"value":"1741-427X","type":"print"},{"value":"1741-4288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013]]}}}