{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,4]],"date-time":"2026-05-04T20:36:08Z","timestamp":1777926968021,"version":"3.51.4"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100007774","name":"Instituto de Ciencia y Tecnolog\u00eda del Distrito Federal, Mexico","doi-asserted-by":"crossref","award":["236\/2010"],"award-info":[{"award-number":["236\/2010"]}],"id":[{"id":"10.13039\/100007774","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007774","name":"Instituto de Ciencia y Tecnolog\u00eda del Distrito Federal, Mexico","doi-asserted-by":"crossref","award":["69081"],"award-info":[{"award-number":["69081"]}],"id":[{"id":"10.13039\/100007774","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Consejo Nacional de Ciencia y Tecnolog\u00eda, Mexico","award":["236\/2010"],"award-info":[{"award-number":["236\/2010"]}]},{"name":"Consejo Nacional de Ciencia y Tecnolog\u00eda, Mexico","award":["69081"],"award-info":[{"award-number":["69081"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>The dog is considered the main domestic reservoir for<jats:italic>Trypanosoma cruzi<\/jats:italic>infection and a suitable experimental animal model to study the pathological changes during the course of Chagas disease (CD). Vaccine development is one of CD prevention methods to protect people at risk. Two plasmids containing genes encoding a<jats:italic>trans<\/jats:italic>-sialidase protein (TcSP) and an amastigote-specific glycoprotein (<jats:italic>TcSSP4<\/jats:italic>) were used as DNA vaccines in a canine model. Splenomegaly was not found in either of the recombinant plasmid-immunized groups; however, cardiomegaly was absent in animals immunized only with the plasmid containing the<jats:italic>TcSSP4<\/jats:italic>gene. The inflammation of subendocardial and myocardial tissues was prevented only with the immunization with<jats:italic>TcSSP4<\/jats:italic>gene. In conclusion, the vaccination with these genes has a partial protective effect on the enlargement of splenic and cardiac tissues during the chronic CD and on microscopic hearth damage, since both plasmids prevented splenomegaly but only one avoided cardiomegaly, and the lesions in heart tissue of dog immunized with plasmid containing the<jats:italic>TcSSP4<\/jats:italic>gene covered only subepicardial tissue.<\/jats:p>","DOI":"10.1155\/2013\/826570","type":"journal-article","created":{"date-parts":[[2013,9,20]],"date-time":"2013-09-20T00:48:10Z","timestamp":1379638090000},"page":"1-8","source":"Crossref","is-referenced-by-count":10,"title":["Effect of the Plasmid-DNA Vaccination on Macroscopic and Microscopic Damage Caused by the Experimental Chronic<i>Trypanosoma cruzi<\/i>Infection in the Canine Model"],"prefix":"10.1155","volume":"2013","author":[{"given":"Olivia","family":"Rodr\u00edguez-Morales","sequence":"first","affiliation":[{"name":"Department of Molecular Biology, Instituto Nacional de Cardiolog\u00eda \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, 14080 Mexico City, DF, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia C.","family":"Carrillo-S\u00e1nchez","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Instituto Nacional de Cardiolog\u00eda \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, 14080 Mexico City, DF, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Humberto","family":"Garc\u00eda-Mendoza","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Instituto Nacional de Cardiolog\u00eda \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, 14080 Mexico City, DF, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Aranda-Fraustro","sequence":"additional","affiliation":[{"name":"Department of Pathological Anatomy, Instituto Nacional de Cardiolog\u00eda \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, 14080 Mexico City, DF, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha A.","family":"Ballinas-Verdugo","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Instituto Nacional de Cardiolog\u00eda \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, 14080 Mexico City, DF, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ricardo","family":"Alejandre-Aguilar","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Escuela Nacional de Ciencias Biol\u00f3gicas del I.P.N., Prolongaci\u00f3n de Carpio y Plan de Ayala, Col. Sto. Tom\u00e1s, Miguel Hidalgo, 11340 Mexico City, DF, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Luis","family":"Rosales-Encina","sequence":"additional","affiliation":[{"name":"Department of Infectomics and Molecular Pathogenesis, Centro de Investigaci\u00f3n y de Estudios Avanzados del I.P.N., Avenida Instituto Polit\u00e9cnico Nacional No. 2508, Col. San Pedro Zacatenco, Gustavo A. Madero, 07360 Mexico City, DF, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5074-2473","authenticated-orcid":true,"given":"Maite","family":"Vallejo","sequence":"additional","affiliation":[{"name":"Department of Sociomedical Research, Instituto Nacional de Cardiolog\u00eda \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, 14080 Mexico City, DF, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minerva","family":"Arce-Fonseca","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology, Instituto Nacional de Cardiolog\u00eda \u201cIgnacio Ch\u00e1vez\u201d, Juan Badiano No. 1, Col. Secci\u00f3n XVI, Tlalpan, 14080 Mexico City, DF, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"51\/52","key":"1","first-page":"509","volume":"87","year":"2012","journal-title":"Weekly Epidemiological Record"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-8932(09)73071-4"},{"issue":"2","key":"3","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1016\/j.actatropica.2013.04.007","volume":"127","year":"2013","journal-title":"Acta Tropica"},{"issue":"1","key":"4","first-page":"16","volume":"26","year":"2001","journal-title":"Bioquimia"},{"issue":"1","key":"5","first-page":"39","volume":"46","year":"2004","journal-title":"Salud P\u00fablica de M\u00e9xico"},{"issue":"4","key":"6","first-page":"624","volume":"13","year":"2006","journal-title":"Emerging Infectious Diseases"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2007.0224"},{"issue":"3","key":"8","doi-asserted-by":"crossref","first-page":"390","DOI":"10.4269\/ajtmh.2009.81.390","volume":"81","year":"2009","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"issue":"1","key":"9","first-page":"11","volume":"16","year":"2008","journal-title":"Revista M\u00e9dica"},{"issue":"4","key":"10","doi-asserted-by":"crossref","first-page":"521","DOI":"10.2460\/ajvr.1991.53.04.521","volume":"53","year":"1992","journal-title":"American Journal of Veterinary Research"},{"issue":"1","key":"11","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1590\/S0074-02761992000100011","volume":"87","year":"1992","journal-title":"Memorias do Instituto Oswaldo Cruz"},{"issue":"4","key":"12","doi-asserted-by":"crossref","first-page":"339","DOI":"10.1590\/S0037-86822002000400010","volume":"35","year":"2002","journal-title":"Revista da Sociedade Brasileira de Medicina Tropical"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-001-0542-4"},{"issue":"5","key":"14","doi-asserted-by":"crossref","first-page":"273","DOI":"10.1590\/S0036-46652002000500008","volume":"44","year":"2002","journal-title":"Revista do Instituto de Medicina Tropical de Sao Paulo"},{"issue":"4","key":"15","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1590\/S0074-02762002000400006","volume":"97","year":"2002","journal-title":"Memorias do Instituto Oswaldo Cruz"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2008.03.003"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1428.098"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2009.01.004"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0001050"},{"key":"20","doi-asserted-by":"crossref","first-page":"S463","DOI":"10.1590\/S0036-36342009000900012","volume":"51","year":"2009","journal-title":"Salud P\u00fablica de M\u00e9xico"},{"issue":"9","key":"21","doi-asserted-by":"crossref","first-page":"1230","DOI":"10.7150\/ijbs.7.1230","volume":"7","year":"2011","journal-title":"International Journal of Biological Sciences"},{"issue":"1","key":"22","doi-asserted-by":"crossref","first-page":"32","DOI":"10.1111\/pim.12017","volume":"35","year":"2013","journal-title":"Parasite Immunology"},{"key":"23","series-title":"pp. 1\u2013109","year":"2002"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-43-79"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2010.0231"},{"key":"26","series-title":"pp. 1\u201358","year":"1999"},{"key":"27","series-title":"pp. 1\u201317","year":"1995"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1996.0074"},{"key":"29","year":"1992"},{"key":"30","year":"1999"},{"issue":"2","key":"31","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1590\/S0074-02762007000200003","volume":"102","year":"2007","journal-title":"Memorias do Instituto Oswaldo Cruz"},{"issue":"12","key":"32","doi-asserted-by":"crossref","first-page":"2033","DOI":"10.2460\/ajvr.1991.52.12.2033","volume":"52","year":"1991","journal-title":"American Journal of Veterinary Research"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/890672"},{"issue":"9","key":"34","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.7150\/ijbs.7.1357","volume":"7","year":"2011","journal-title":"International Journal of Biological Sciences"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1186\/1297-9716-44-15"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1100\/2012\/635169"},{"key":"37","first-page":"87","volume":"11","year":"2007","journal-title":"Salus Revista de la Facultad de Ciencias de la Salud, Universidad de Carabobo"},{"issue":"6","key":"38","doi-asserted-by":"crossref","first-page":"958","DOI":"10.4269\/ajtmh.2001.65.958","volume":"65","year":"2001","journal-title":"The American Journal of Tropical Medicine and Hygiene"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.10.5547-5555.2002"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/826570.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/826570.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/826570.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,18]],"date-time":"2024-05-18T00:19:04Z","timestamp":1715991544000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2013\/826570\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":39,"alternative-id":["826570","826570"],"URL":"https:\/\/doi.org\/10.1155\/2013\/826570","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013]]}}}