{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T18:58:23Z","timestamp":1790881103503,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>This study focused on the therapeutic effect of a propolis SLNC 106<jats:sup><jats:italic>PI<\/jats:italic><\/jats:sup>extract on experimental colitis. Wistar adult rats received 0.8\u2009mL rectal dose of one of the following solutions: saline (group S), 20\u2009mg TNBS in 50% ethanol (group TNBS), 20\u2009mg TNBS in 50% ethanol and propolis extract in saline (group TNBS-P), propolis extract in saline (group SP), and 20\u2009mg TNBS in 50% ethanol and 50\u2009mg\/kg mesalazine (group TNBS-M). The animals were euthanized 7 or 14 days after the colitis induction. Samples of the distal colon were harvested for the analysis of myeloperoxidase (MPO) enzyme activity and for morphometric analysis in paraffin-embedded histological sections with hematoxylin-eosin or histochemical staining. The animals treated with TNBS exhibited the typical clinical signs of colitis. Increased MPO activity confirmed the presence of inflammation. TNBS induced the development of megacolon, ulceration, transmural inflammatory infiltrate, and thickened bowel walls. Treatment with propolis moderately reduced the inflammatory response, decreased the number of cysts and abscesses, inhibited epithelial proliferation, and increased the number of goblet cells. The anti-inflammatory activity of the propolis SLNC 106 extract was confirmed by the reductions in both the inflammatory infiltrate and the number of cysts and abscesses in the colon mucosa.<\/jats:p>","DOI":"10.1155\/2013\/853976","type":"journal-article","created":{"date-parts":[[2013,9,11]],"date-time":"2013-09-11T21:01:54Z","timestamp":1378933314000},"page":"1-11","source":"Crossref","is-referenced-by-count":20,"title":["Use of Propolis Hydroalcoholic Extract to Treat Colitis Experimentally Induced in Rats by 2,4,6-Trinitrobenzenesulfonic Acid"],"prefix":"10.1155","volume":"2013","author":[{"given":"Cely Cristina Martins","family":"Gon\u00e7alves","sequence":"first","affiliation":[{"name":"Laboratory of Animal Histology, Department of Morphological Sciences, State University of Maring\u00e1, 87020-900 Maring\u00e1, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luzmarina","family":"Hernandes","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Histology, Department of Morphological Sciences, State University of Maring\u00e1, 87020-900 Maring\u00e1, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciomar Aparecida","family":"Bersani-Amado","sequence":"additional","affiliation":[{"name":"Laboratory of Inflammation, Department of Pharmacology and Therapeutics, State University of Maring\u00e1, 87020-900 Maring\u00e1, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selma Lucy","family":"Franco","sequence":"additional","affiliation":[{"name":"Laboratory of Phytotherapy and Apitherapy Development, Department of Pharmacy, State University of Maring\u00e1, 87020-900 Maring\u00e1, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joaquim Felipe de Souza","family":"Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Histology, Department of Morphological Sciences, State University of Maring\u00e1, 87020-900 Maring\u00e1, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7486-2581","authenticated-orcid":true,"given":"Maria Raquel Mar\u00e7al","family":"Natali","sequence":"additional","affiliation":[{"name":"Laboratory of Animal Histology, Department of Morphological Sciences, State University of Maring\u00e1, 87020-900 Maring\u00e1, PR, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","first-page":"108","volume-title":"Inflammatory bowel disease","year":"2002"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(98)70381-6"},{"issue":"3","key":"3","first-page":"390","volume":"7","year":"2006","journal-title":"Nature Clinical Practice Gastroenterology and Hepatology"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.37050"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1124\/mol.107.034041"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1139\/Y11-080"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2007.10.053"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nep081"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.08.035"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2010.01.024"},{"issue":"3","key":"11","first-page":"915","volume":"299","year":"2001","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmc.2006.03.015"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2006.03.045"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2004.01.013"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(98)00131-7"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.aca.2009.01.019"},{"issue":"2","key":"17","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1051\/apido:19950202","volume":"26","year":"1995","journal-title":"Apidologie"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nem177"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(02)00298-X"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2005.01.042"},{"issue":"2-3","key":"21","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.jep.2004.06.006","volume":"94","year":"2004","journal-title":"Journal of Ethnopharmacology"},{"issue":"17","key":"22","first-page":"48","volume":"11","year":"1999","journal-title":"Infarma"},{"issue":"3","key":"23","doi-asserted-by":"crossref","first-page":"795","DOI":"10.1016\/0016-5085(89)90904-9","volume":"96","year":"1989","journal-title":"Gastroenterology"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejps.2005.12.005"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011032328064"},{"issue":"3","key":"26","doi-asserted-by":"crossref","first-page":"401","DOI":"10.1242\/jcs.102.3.401","volume":"102","year":"1992","journal-title":"Journal of Cell Science"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-3659(01)00532-6"},{"issue":"6","key":"28","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.1016\/0016-5085(84)90202-6","volume":"87","year":"1984","journal-title":"Gastroenterology"},{"issue":"2","key":"29","doi-asserted-by":"crossref","first-page":"155","DOI":"10.3109\/00365529309096063","volume":"28","year":"1993","journal-title":"Scandinavian Journal of Gastroenterology"},{"key":"30","year":"1984"},{"issue":"1-2","key":"31","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/0014-4827(67)90237-6","volume":"47","year":"1967","journal-title":"Experimental Cell Research"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1080\/13880200701575411"},{"issue":"5","key":"33","doi-asserted-by":"crossref","first-page":"493","DOI":"10.1590\/S0074-02762006000500002","volume":"101","year":"2006","journal-title":"Memorias do Instituto Oswaldo Cruz"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1023\/A:1012313424144"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200215695"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2005.00703.x"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1007\/BF02877715"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/S0952-3278(96)90129-5"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(96)01466-3"},{"issue":"5","key":"40","doi-asserted-by":"crossref","first-page":"496","DOI":"10.1248\/bpb.20.496","volume":"20","year":"1997","journal-title":"Biological and Pharmaceutical Bulletin"},{"issue":"2","key":"41","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1590\/S0104-79302000000200006","volume":"6","year":"2000","journal-title":"Journal of Venomous Animals and Toxins"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1329.062"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.17.9090"},{"issue":"4","key":"44","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1097\/00054725-200307000-00005","volume":"9","year":"2003","journal-title":"Inflammatory Bowel Diseases"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2003.11.011"},{"issue":"2","key":"46","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.intimp.2013.04.004","volume":"16","year":"2013","journal-title":"International Immunopharmacology"},{"issue":"4","key":"47","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1016\/S0002-9440(10)65207-8","volume":"155","year":"1999","journal-title":"American Journal of Pathology"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1007\/s00428-009-0795-x"},{"key":"49","volume-title":"Inflammatory bowel disease: ulcerative colitis and Crohn\u2019s disease","year":"1998"},{"issue":"7","key":"50","doi-asserted-by":"crossref","first-page":"1578","DOI":"10.1111\/j.1572-0241.2003.07542.x","volume":"98","year":"2003","journal-title":"American Journal of Gastroenterology"},{"key":"51","doi-asserted-by":"publisher","DOI":"10.1016\/j.suc.2007.03.001"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21288"},{"key":"53","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2982.1999.00165.x"},{"issue":"1","key":"54","doi-asserted-by":"crossref","first-page":"68","DOI":"10.1097\/00054725-200102000-00014","volume":"7","year":"2001","journal-title":"Inflammatory Bowel Diseases"},{"issue":"12","key":"55","doi-asserted-by":"crossref","first-page":"2523","DOI":"10.1023\/A:1026651408998","volume":"44","year":"1999","journal-title":"Digestive Disease and Sciences"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015931128583"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.3892\/or-00000932"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1016\/j.etp.2007.11.009"}],"container-title":["Evidence-Based Complementary and Alternative Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2013\/853976.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2013\/853976.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/ecam\/2013\/853976.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,6]],"date-time":"2022-03-06T02:17:27Z","timestamp":1646533047000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/ecam\/2013\/853976\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":58,"alternative-id":["853976","853976"],"URL":"https:\/\/doi.org\/10.1155\/2013\/853976","relation":{},"ISSN":["1741-427X","1741-4288"],"issn-type":[{"value":"1741-427X","type":"print"},{"value":"1741-4288","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013]]}}}