{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:49:49Z","timestamp":1787017789168,"version":"build-2736575974"},"reference-count":39,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>\n                    <jats:italic>Giardia duodenalis<\/jats:italic>\n                    is a small intestinal protozoan parasite of several terrestrial vertebrates. This work aims to assess the genotypic variability of\n                    <jats:italic>Giardia duodenalis<\/jats:italic>\n                    isolates from cattle, sheep and pigs in the Southeast of Brazil, by comparing the standard characterization between glutamate dehydrogenase (\n                    <jats:italic>gdh<\/jats:italic>\n                    ) and triose phosphate isomerase (\n                    <jats:italic>tpi<\/jats:italic>\n                    ) primers. Fecal samples from the three groups of animals were analyzed using the zinc sulphate centrifugal flotation technique. Out of 59 positive samples, 30 were from cattle, 26 from sheep and 3 from pigs. Cyst pellets were stored and submitted to PCR and nested-PCR reactions with\n                    <jats:italic>gdh<\/jats:italic>\n                    and\n                    <jats:italic>tpi<\/jats:italic>\n                    primers. Fragment amplification of\n                    <jats:italic>gdh<\/jats:italic>\n                    and\n                    <jats:italic>tpi<\/jats:italic>\n                    genes was observed in 25 (42.4%) and 36 (61.0%) samples, respectively. Regarding the sequencing, 24 sequences were obtained with\n                    <jats:italic>gdh<\/jats:italic>\n                    and 20 with\n                    <jats:italic>tpi<\/jats:italic>\n                    . For both genes, there was a prevalence of E specific species assemblage, although some isolates have been identified as A and B, by the\n                    <jats:italic>tpi<\/jats:italic>\n                    sequencing. This has also shown a larger number of heterogeneous sequences, which have been attribute to mixed infections between assemblages B and E. The largest variability of inter-assemblage associated to the frequency of heterogeneity provided by\n                    <jats:italic>tpi<\/jats:italic>\n                    sequencing reinforces the polymorphic nature of this gene and makes it an excellent target for studies on molecular epidemiology.\n                  <\/jats:p>","DOI":"10.1155\/2013\/875048","type":"journal-article","created":{"date-parts":[[2013,11,6]],"date-time":"2013-11-06T16:08:28Z","timestamp":1383754108000},"page":"1-9","source":"Crossref","is-referenced-by-count":21,"title":["Performance of Glutamate Dehydrogenase and Triose Phosphate Isomerase Genes in the Analysis of Genotypic Variability of Isolates of\n                    <i>Giardia duodenalis<\/i>\n                    from Livestocks"],"prefix":"10.1155","volume":"2013","author":[{"given":"Nat\u00e1lia M. N.","family":"Fava","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, 1720 Avenida Par\u00e1, Umuarama Campus, 38400-902 Uberl\u00e2ndia, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo M.","family":"Soares","sequence":"additional","affiliation":[{"name":"Departamento de Veterin\u00e1ria Preventiva e Sa\u00fade Animal, Faculdade de Medicina Veterin\u00e1ria e Zootecnia, Universidade de S\u00e3o Paulo, Avenida Prof. Dr. Orlando Marques de Paiva, 87-Cidade Universit\u00e1ria, 05508-270 S\u00e3o Paulo, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luana A. M.","family":"Scalia","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, 1720 Avenida Par\u00e1, Umuarama Campus, 38400-902 Uberl\u00e2ndia, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evanguedes","family":"Kalapothakis","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia e Marcadores Moleculares, Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de MG, 6627 Avenida Ant\u00f4nio Carlos, Pampulha Campus, 31270-901, Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabella F.","family":"Pena","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia e Marcadores Moleculares, Departamento de Biologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de MG, 6627 Avenida Ant\u00f4nio Carlos, Pampulha Campus, 31270-901, Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos U.","family":"Vieira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, 1720 Avenida Par\u00e1, Umuarama Campus, 38400-902 Uberl\u00e2ndia, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elaine S. M.","family":"Faria","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, 1720 Avenida Par\u00e1, Umuarama Campus, 38400-902 Uberl\u00e2ndia, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria J.","family":"Cunha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Parasitologia, Instituto de Ci\u00eancias Biom\u00e9dicas, Universidade Federal de Uberl\u00e2ndia, 1720 Avenida Par\u00e1, Umuarama Campus, 38400-902 Uberl\u00e2ndia, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Talles R.","family":"Couto","sequence":"additional","affiliation":[{"name":"M\u00e9dico Veterin\u00e1rio\u2014Instituto de Ci\u00eancias Biom\u00e9dicas, 1720 Avenida Par\u00e1, Umuarama Campus, 38400-902 Uberl\u00e2ndia, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e1rcia 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