{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T05:59:52Z","timestamp":1789106392177,"version":"build-2803163510"},"reference-count":24,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004386","name":"Universiti Malaya","doi-asserted-by":"publisher","award":["PV046\/2011B"],"award-info":[{"award-number":["PV046\/2011B"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004386","name":"Universiti Malaya","doi-asserted-by":"publisher","award":["UM.C\/625\/1\/HIR\/MOHE\/02"],"award-info":[{"award-number":["UM.C\/625\/1\/HIR\/MOHE\/02"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004386","name":"Universiti Malaya","doi-asserted-by":"publisher","award":["PV046\/2011B"],"award-info":[{"award-number":["PV046\/2011B"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004386","name":"Universiti Malaya","doi-asserted-by":"publisher","award":["UM.C\/625\/1\/HIR\/MOHE\/02"],"award-info":[{"award-number":["UM.C\/625\/1\/HIR\/MOHE\/02"]}],"id":[{"id":"10.13039\/501100004386","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p><jats:italic>Staphylococcus aureus<\/jats:italic>, including methicillin-resistant<jats:italic>S. aureus<\/jats:italic>(MRSA), is an important human pathogen that produces a variety of toxins and causes a wide range of infections, including soft-tissue infections, bacteremia, and staphylococcal food poisoning. A loop-mediated isothermal amplification (LAMP) assay targeting the<jats:italic>arcC<\/jats:italic>gene of<jats:italic>S. aureus<\/jats:italic>was developed and evaluated with 119<jats:italic>S. aureus<\/jats:italic>and 25 non-<jats:italic>S. aureus<\/jats:italic>strains. The usefulness of the assay was compared with the PCR method that targets<jats:italic>spa<\/jats:italic>and<jats:italic>arcC<\/jats:italic>genes. The optimal temperature for the LAMP assay was 58.5\u00b0C with a detection limit of 2.5\u2009ng\/<jats:italic>\u03bc<\/jats:italic>L and 10<jats:sup>2<\/jats:sup>\u2009CFU\/mL when compared to 12.5\u2009ng\/<jats:italic>\u03bc<\/jats:italic>L and 10<jats:sup>3<\/jats:sup>\u2009CFU\/mL for PCR (<jats:italic>spa<\/jats:italic>and<jats:italic>arcC<\/jats:italic>). Both LAMP and PCR assays were 100% specific, 100% sensitive, 100% positive predictive value (PPV), and 100% negative predictive value (NPV). When tested on 30 spiked blood specimens (21 MRSA, eight non-<jats:italic>S. aureus<\/jats:italic>and one negative control), the performance of LAMP and PCR was comparable: 100% specific, 100% sensitive, 100% PPV, and 100% NPV. In conclusion, the LAMP assay was equally specific with a shorter detection time when compared to PCR in the identification of<jats:italic>S. aureus<\/jats:italic>. The LAMP assay is a promising alternative method for the rapid identification of<jats:italic>S. aureus<\/jats:italic>and could be used in resource-limited laboratories and fields.<\/jats:p>","DOI":"10.1155\/2013\/895816","type":"journal-article","created":{"date-parts":[[2013,1,15]],"date-time":"2013-01-15T21:11:02Z","timestamp":1358284262000},"page":"1-5","source":"Crossref","is-referenced-by-count":37,"title":["Loop-Mediated Isothermal Amplification Assay for the Rapid Detection of<i>Staphylococcus aureus<\/i>"],"prefix":"10.1155","volume":"2013","author":[{"given":"King Ting","family":"Lim","sequence":"first","affiliation":[{"name":"Institute of Biological Science, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia"},{"name":"Laboratory of Biomedical Science and Molecular Microbiology, Institute of Graduate Studies, University of Malaya, 50603 Kuala Lumpur, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy Shuan 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