{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T14:21:48Z","timestamp":1787062908133,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>\n                    We report a rapid purification method using one-step chromatography of SVSP Rhombeobin (LMR-47) from\n                    <jats:italic>Lachesis muta rhombeata<\/jats:italic>\n                    venom and its procoagulant activities and effects on platelet aggregation. The venom was fractionated by a single chromatographic step in RP-HPLC on a C8 Discovery BIO Wide Pore, showing high degree of molecular homogeneity with molecular mass of 47035.49\u2009Da. Rhombeobin showed amidolytic activity upon BA\n                    <jats:italic>\u03c1<\/jats:italic>\n                    NA, with a broad optimum pH (7\u201310) and was stable in solution up to 60\u00b0C. The amidolytic activity was inhibited by serine proteinase inhibitors and reducing agents, but not chelating agents. Rhombeobin showed high coagulant activity on mice plasma and bovine fibrinogen. The deduced amino acid sequence of Rhombeobin showed homology with other SVSPs, especially with LM-TL (\n                    <jats:italic>L. m. muta<\/jats:italic>\n                    ) and Gyroxin (\n                    <jats:italic>C. d. terrificus<\/jats:italic>\n                    ). Rhombeobin acts,\n                    <jats:italic>in vitro<\/jats:italic>\n                    , as a strong procoagulant enzyme on mice citrated plasma, shortening the APTT and PT tests in adose-dependent manner. The protein showed, \u201c\n                    <jats:italic>ex vivo<\/jats:italic>\n                    \u201d, a strong defibrinogenating effect with 1\u2009\u00b5g\/animal. Lower doses activated the intrinsic and extrinsic coagulation pathways and impaired the platelet aggregation induced by ADP. Thus, this is the first report of a venom component that produces a venom-induced consumptive coagulopathy (VICC).\n                  <\/jats:p>","DOI":"10.1155\/2013\/903292","type":"journal-article","created":{"date-parts":[[2013,8,24]],"date-time":"2013-08-24T17:02:41Z","timestamp":1377363761000},"page":"1-12","source":"Crossref","is-referenced-by-count":19,"title":["Rapid Purification and Procoagulant and Platelet Aggregating Activities of Rhombeobin: A Thrombin-Like\/Gyroxin-Like Enzyme from\n                    <i>Lachesis muta rhombeata<\/i>\n                    Snake Venom"],"prefix":"10.1155","volume":"2013","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7638-8847","authenticated-orcid":true,"given":"Frank Denis","family":"Torres-Huaco","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Institute of Biology, University of Campinas (UNICAMP), P.O. Box 6109, 13083-970 Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9413-0572","authenticated-orcid":true,"given":"Cl\u00e1udio C.","family":"Werneck","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Biology, University of Campinas (UNICAMP), P.O. Box 6109, 13083-970 Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9896-4630","authenticated-orcid":true,"given":"Cristina Pontes","family":"Vicente","sequence":"additional","affiliation":[{"name":"Department of Structural and Functional Biology, Institute of Biology, State University of Campinas (UNICAMP), P.O. Box 6109, 13083-865 Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3848-5192","authenticated-orcid":true,"given":"Talita","family":"Vassequi-Silva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Biology, University of Campinas (UNICAMP), P.O. Box 6109, 13083-970 Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5730-5540","authenticated-orcid":true,"given":"Ana Cl\u00e1udia Coelho","family":"Nery-Diez","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Biology, University of Campinas (UNICAMP), P.O. Box 6109, 13083-970 Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Camila B.","family":"Mendes","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, State University of Campinas (UNICAMP), P.O. Box 6109, 13083-887 Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2201-8247","authenticated-orcid":true,"given":"Edson","family":"Antunes","sequence":"additional","affiliation":[{"name":"Department of Pharmacology, State University of Campinas (UNICAMP), P.O. Box 6109, 13083-887 Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S\u00e9rgio","family":"Marangoni","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Biology, University of Campinas (UNICAMP), P.O. Box 6109, 13083-970 Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniela C. S.","family":"Damico","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, Institute of Biology, University of Campinas (UNICAMP), P.O. Box 6109, 13083-970 Campinas, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1006\/bijl.1997.0162"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(96)00142-0"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(98)00268-2"},{"key":"4","year":"2001"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.04.011"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(98)00089-0"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1995.1227"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.02.020"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(95)00159-X"},{"issue":"8","key":"10","doi-asserted-by":"crossref","first-page":"809","DOI":"10.1023\/A:1026372018547","volume":"16","year":"1997","journal-title":"Journal of Protein Chemistry"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(92)90022-W"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(93)80205-9"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2009.03.022"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2005.04.018"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1995.1208"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2007.10.005"},{"issue":"16","key":"32","doi-asserted-by":"crossref","first-page":"7628","DOI":"10.1016\/S0021-9258(18)68544-8","volume":"263","year":"1988","journal-title":"The Journal of Biological Chemistry"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3540161"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(01)00177-7"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"issue":"1-2","key":"13","doi-asserted-by":"crossref","first-page":"248","DOI":"10.1016\/0003-2697(76)90527-3","volume":"72","year":"1976","journal-title":"Analytical Biochemistry"},{"issue":"2","key":"14","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1016\/0003-9861(61)90145-X","volume":"95","year":"1961","journal-title":"Archives of Biochemistry and Biophysics"},{"issue":"6","key":"15","first-page":"949","volume":"61","year":"1983","journal-title":"Bulletin of the World Health Organization"},{"key":"11","doi-asserted-by":"crossref","first-page":"773","DOI":"10.1016\/j.toxicon.2012.06.010","volume":"60","year":"2012","journal-title":"Toxicon"},{"issue":"3","key":"16","doi-asserted-by":"crossref","first-page":"235","DOI":"10.1016\/0041-0101(80)90002-1","volume":"18","year":"1980","journal-title":"Toxicon"},{"key":"17","volume-title":"Isolation and characterization of gyroxin from Crotalus durissus terriftcus venom","year":"1978"},{"issue":"6","key":"18","first-page":"509","volume":"64","year":"2004","journal-title":"Medicina B"},{"issue":"7","key":"20","doi-asserted-by":"crossref","first-page":"1843","DOI":"10.1021\/bi00173a030","volume":"33","year":"1994","journal-title":"Biochemistry"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2005.12.033"},{"issue":"3","key":"22","first-page":"438","volume":"83","year":"2008","journal-title":"Thrombosis and Haemostasis"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0977"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(02)00140-X"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2007.02.018"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2003.08.001"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(92)80559-Y"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-003-3325-z"},{"issue":"23","key":"37","doi-asserted-by":"crossref","first-page":"12230","DOI":"10.1016\/S0021-9258(19)86454-2","volume":"254","year":"1979","journal-title":"The Journal of Biological Chemistry"},{"issue":"33","key":"38","doi-asserted-by":"crossref","first-page":"17471","DOI":"10.1016\/S0021-9258(19)77860-0","volume":"263","year":"1988","journal-title":"The Journal of Biological Chemistry"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(98)00232-5"},{"issue":"16","key":"40","doi-asserted-by":"crossref","first-page":"3570","DOI":"10.1021\/bi00583a021","volume":"18","year":"1979","journal-title":"Biochemistry"},{"issue":"1","key":"41","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1111\/j.1432-1033.1997.00121.x","volume":"247","year":"1997","journal-title":"European Journal of Biochemistry"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1055\/s-0029-1214152"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.02.030"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1254053"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(00)01803-2"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(95)00035-K"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/903292.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/903292.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2013\/903292.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T15:40:41Z","timestamp":1646408441000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2013\/903292\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013]]},"references-count":46,"alternative-id":["903292","903292"],"URL":"https:\/\/doi.org\/10.1155\/2013\/903292","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013]]}}}