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The nucleoside analogue acyclovir reduces clinical symptoms of the three human alpha herpesviruses, HSV-1, HSV-2, and VZV. Long-term administration of acyclovir (ACV) can reduce the frequency and severity of reactivation, but its low bioavailability and short half-life require a daily drug regimen. Our lab is working to develop a subcutaneous delivery system to provide long-lasting, sustained release of ACV. Previously, we demonstrated that an implantable silicone (MED-4050) device, impregnated with ACV protected against HSV-1 both<jats:italic>in vitro<\/jats:italic>and<jats:italic>in vivo<\/jats:italic>. Here, we extend our<jats:italic>in vitro<\/jats:italic>observations to include protection against both HSV-2 and VZV. We also demonstrate protection against HSV-2<jats:italic>in vitro<\/jats:italic>using MED-4750, a silicone polymer designed for long-term use in humans. When release of ACV from MED-4750 is quantitated on a daily basis, an initial burst of 5 days is observed, followed by a long period of slow release with near-zero-order kinetics, with an average daily release of 1.3923 \u00b1 0.5908\u2009<jats:italic>\u03bc<\/jats:italic>g ACV over days 20\u201360. Development of a slow-release implant has the potential to significantly impact the treatment of human alpha herpesvirus infections.<\/jats:p>","DOI":"10.1155\/2013\/915159","type":"journal-article","created":{"date-parts":[[2013,8,4]],"date-time":"2013-08-04T17:01:28Z","timestamp":1375635688000},"page":"1-9","source":"Crossref","is-referenced-by-count":1,"title":["Silicone-Acyclovir Controlled Release Devices Suppress Primary Herpes Simplex Virus-2 and Varicella Zoster Virus Infections<i>In Vitro<\/i>"],"prefix":"10.1155","volume":"2013","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9629-5373","authenticated-orcid":true,"given":"Carol L.","family":"Berkower","sequence":"first","affiliation":[{"name":"Towson University Herpes Virus Lab, Department of Biological Sciences, Towson University, Towson, MD 21252, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6452-9542","authenticated-orcid":true,"given":"Nicole 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