{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T23:51:44Z","timestamp":1787615504987,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>Antibiotic resistance continues to pose a significant problem in the management of bacterial infections, despite advances in antimicrobial chemotherapy and supportive care. Here, we suggest a simple, inexpensive, and easy-to-perform assay to screen antimicrobial compounds from natural products or synthetic chemical libraries for their potential to work in tandem with the available antibiotics against multiple drug-resistant bacteria. The aqueous extract of<jats:italic>Juglans regia<\/jats:italic>tree bark was tested against representative multiple drug-resistant bacteria in the aforementioned assay to determine whether it potentiates the activity of selected antibiotics. The aqueous extract of<jats:italic>J. regia<\/jats:italic>bark was added to Mueller-Hinton agar, followed by a lawn of multiple drug-resistant bacteria,<jats:italic>Salmonella typhi<\/jats:italic>or enteropathogenic<jats:italic>E. coli<\/jats:italic>. Next, filter paper discs impregnated with different classes of antibiotics were placed on the agar surface. Bacteria incubated with extract or antibiotics alone were used as controls. The results showed a significant increase (&gt;30%) in the zone of inhibition around the aztreonam, cefuroxime, and ampicillin discs compared with bacteria incubated with the antibiotics\/extract alone. In conclusion, our assay is able to detect either synergistic or additive action of<jats:italic>J. regia<\/jats:italic>extract against multiple drug-resistant bacteria when tested with a range of antibiotics.<\/jats:p>","DOI":"10.1155\/2013\/927323","type":"journal-article","created":{"date-parts":[[2013,6,20]],"date-time":"2013-06-20T17:20:33Z","timestamp":1371748833000},"page":"1-4","source":"Crossref","is-referenced-by-count":13,"title":["A Simple Assay to Screen Antimicrobial Compounds Potentiating the Activity of Current Antibiotics"],"prefix":"10.1155","volume":"2013","author":[{"given":"Junaid","family":"Iqbal","sequence":"first","affiliation":[{"name":"Department of Biological and Biomedical Sciences, Aga Khan University, Stadium Road, Karachi 74800, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7229-9166","authenticated-orcid":true,"given":"Ruqaiyyah","family":"Siddiqui","sequence":"additional","affiliation":[{"name":"Department of Biological and Biomedical Sciences, Aga Khan University, Stadium Road, Karachi 74800, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shahana Urooj","family":"Kazmi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Karachi, Karachi 75270, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7667-8553","authenticated-orcid":true,"given":"Naveed Ahmed","family":"Khan","sequence":"additional","affiliation":[{"name":"Department of Biological and Biomedical Sciences, Aga Khan University, Stadium Road, Karachi 74800, 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