{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T12:10:50Z","timestamp":1764936650846},"reference-count":21,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>CD109 gene encodes a glycosylphosphatidylinositol-linked glycoprotein found in a subset of platelets and endothelial cell, and human platelet antigen (HPA) 15 is found on CD109. We evaluated the HPA genotype and\/or the CD109 mRNA expression on two peripheral blood stem cells (PBSC), two peripheral bloods (PB), 12 granulocyte products, natural killer (NK)-92, B-lymphocyte (CO88BV59-1), K-562 leukemia cell line, human embryonic stem cell (hESC), and human fibroblasts (HF). HPA genotyping was performed by SNaPshot assay and CD109 mRNA expression was evaluated by real-time PCR with SYBR green and melting curve analysis. Genotype HPA-15a\/-15a was found in PBSC#1 and two granulocyte products, and HPA-15a\/-15b was found in PBSC#2, eight granulocyte products, NK-92, K-562, hESC, and HF, and HPA-15b\/-15b was found in two granulocyte products. CD109 mRNA expression was highly increased in HF and increased in CD34+ and CD34\u2212 PBSCs and some granulocyte products, compared to the PB. However, the increase of expression level varied among the PBSC and granulocyte products. The CD109 mRNA expression of NK-92, K-562, hESC, and CO 88BV59-1 was not detected. HPA genotype was evaluated in various cells and the expression of CD109, which contains HPA 15, was different among cell lines and high in HF and PBSCs.<\/jats:p>","DOI":"10.1155\/2013\/946403","type":"journal-article","created":{"date-parts":[[2013,2,12]],"date-time":"2013-02-12T21:02:11Z","timestamp":1360702931000},"page":"1-5","source":"Crossref","is-referenced-by-count":7,"title":["Human Platelet Antigen Genotyping and Expression of CD109 (Human Platelet Antigen 15) mRNA in Various Human Cell Types"],"prefix":"10.1155","volume":"2013","author":[{"given":"Sang Mee","family":"Hwang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea"},{"name":"Department of Laboratory Medicine, Seoul National University Bundang Hospital, 173-82 Gumiro, Bundanggu, Gyeonggido, Seongnam 463-707, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mi Jung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University Bundang Hospital, 173-82 Gumiro, Bundanggu, Gyeonggido, Seongnam 463-707, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ho Eun","family":"Chang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University Bundang Hospital, 173-82 Gumiro, Bundanggu, Gyeonggido, Seongnam 463-707, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun Ji","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea"},{"name":"Department of Laboratory Medicine, Seoul National University Bundang Hospital, 173-82 Gumiro, Bundanggu, Gyeonggido, Seongnam 463-707, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taek Soo","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea"},{"name":"Department of Laboratory Medicine, Seoul National University Bundang Hospital, 173-82 Gumiro, Bundanggu, Gyeonggido, Seongnam 463-707, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eun Young","family":"Song","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kyoung Un","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea"},{"name":"Department of Laboratory Medicine, Seoul National University Bundang Hospital, 173-82 Gumiro, Bundanggu, Gyeonggido, Seongnam 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