{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:47:52Z","timestamp":1787017672628,"version":"build-2736575974"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2013]]},"abstract":"<jats:p>\n                    <jats:italic>Plukenetia volubilis<\/jats:italic>\n                    Linneo, or Sacha inca, is an oleaginous plant from the Euphorbiaceae family. The aim of this work was to perform a chemical and biological analysis of different leaf extracts from\n                    <jats:italic>P. volubilis<\/jats:italic>\n                    such as aqueous extract (AEL), methanol (MEL), ethanol (EEL), chloroform (CEL), and hexane (HEL). Thin layer chromatography analysis revealed the presence of phenolic compounds, steroids, and\/or terpeno\u00eddes. Furthermore, the antioxidant activities were analyzed by\n                    <jats:italic>in vitro<\/jats:italic>\n                    assays and their effects on cell lineages by\n                    <jats:italic>in vivo<\/jats:italic>\n                    assays. The Total Antioxidant Capacity (TCA) was expressed as equivalent ascorbic acid (EEA\/g) and it was observed that the extracts showed values ranging from 59.31 to 97.76\u2009EAA\/g. Furthermore, the DPPH assay values ranged from 62.8% to 88.3%. The cell viability assay showed that the extracts were able to reduce viability from cancer cells such as HeLa and A549 cells. The extracts MEL and HEL (250\u2009\u00b5g\/mL) were able to reduce the proliferation of HeLa cells up to 54.3% and 48.5%, respectively. The flow cytometer results showed that these extracts induce cell death via the apoptosis pathway. On the other hand, the extracts HEL and AEL were able to induce cell proliferation of normal fibroblast 3T3 cells.\n                  <\/jats:p>","DOI":"10.1155\/2013\/950272","type":"journal-article","created":{"date-parts":[[2013,9,18]],"date-time":"2013-09-18T17:03:51Z","timestamp":1379523831000},"page":"1-10","source":"Crossref","is-referenced-by-count":53,"title":["Antioxidant and Antiproliferative Activities of Leaf Extracts from\n                    <i>Plukenetia volubilis<\/i>\n                    Linneo (Euphorbiaceae)"],"prefix":"10.1155","volume":"2013","author":[{"given":"Ana Karina Lima","family":"Nascimento","sequence":"first","affiliation":[{"name":"Departamento de Biologia Celular e Gen\u00e9tica, Centro de Bioci\u00eancias, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, RN, Brazil"},{"name":"Laborat\u00f3rio de Biotecnologia de Pol\u00edmeros Naturais-Biopol, Departamento de Bioqu\u00edmica, Centro de Bioci\u00eancias, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raniere Fagundes","family":"Melo-Silveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Pol\u00edmeros Naturais-Biopol, Departamento de Bioqu\u00edmica, Centro de Bioci\u00eancias, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nednaldo","family":"Dantas-Santos","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Pol\u00edmeros Naturais-Biopol, Departamento de Bioqu\u00edmica, Centro de Bioci\u00eancias, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00falia Morais","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia, Centro de Ci\u00eancias da Sa\u00fade, Universidade Federal do Rio Grande do Norte, 59010-180 Natal, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvana Maria","family":"Zucolotto","sequence":"additional","affiliation":[{"name":"Departamento de Farm\u00e1cia, Centro de Ci\u00eancias da Sa\u00fade, Universidade Federal do Rio Grande do Norte, 59010-180 Natal, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo Alexandre Oliveira","family":"Rocha","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biotecnologia de Pol\u00edmeros Naturais-Biopol, Departamento de Bioqu\u00edmica, Centro de Bioci\u00eancias, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katia Castanho","family":"Scortecci","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Celular e Gen\u00e9tica, Centro de Bioci\u00eancias, Universidade Federal do Rio Grande do Norte, 59072-970 Natal, RN, 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