{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T03:18:31Z","timestamp":1783394311292,"version":"3.54.6"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,12,17]],"date-time":"2013-12-17T00:00:00Z","timestamp":1387238400000},"content-version":"vor","delay-in-days":350,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:p>The diagnosis of periprosthetic joint infection is sometimes straightforward with purulent discharge from the fistula tract communicating to the joint prosthesis. However it is often difficult to differentiate septic from aseptic loosening of prosthesis because of the high culture\u2010negative rates in conventional microbiologic culture. This study used quantitative reverse transcription polymerase chain reaction (RT\u2010qPCR) to amplify bacterial 16S ribosomal RNA in vitro and in 11 clinical samples. The in vitro analysis demonstrated that the RT\u2010qPCR method was highly sensitive with the detection limit of bacterial 16S rRNA being 0.148\u2009pg\/<jats:italic>\u03bc<\/jats:italic>l. Clinical specimens were analyzed using the same protocol. The RT\u2010qPCR was positive for bacterial detection in 8 culture\u2010positive cases (including aerobic, anaerobic, and mycobacteria) and 2 culture\u2010negative cases. It was negative in one case that the final diagnosis was confirmed without infection. The molecular diagnosis of bacterial infection using RT\u2010qPCR to detect bacterial 16S rRNA around a prosthesis correlated well with the clinical findings. Based on the promising clinical results, we were attempting to differentiate bacterial species or drug\u2010resistant strains by using species\u2010specific primers and to detect the persistence of bacteria during the interim period before the second stage reimplantation in a larger scale of clinical subjects.<\/jats:p>","DOI":"10.1155\/2013\/950548","type":"journal-article","created":{"date-parts":[[2013,12,17]],"date-time":"2013-12-17T21:05:01Z","timestamp":1387314301000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["Molecular Diagnosis of Periprosthetic Joint Infection by Quantitative RT\u2010PCR of Bacterial 16S Ribosomal RNA"],"prefix":"10.1155","volume":"2013","author":[{"given":"Mel S.","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Hsin","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Su-Chin","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pang-Hsin","family":"Hsieh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hsin-Nung","family":"Shih","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steve W. N.","family":"Ueng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gwo-Bin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,12,17]]},"reference":[{"key":"e_1_2_6_1_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.D.02937"},{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2007.10.017"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-011-2102-9"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-198365080-00008"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.2106\/00004623-199510000-00015"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/00003086-200403000-00010"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2004.12.055"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.TA.0000130757.53559.BF"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1054\/arth.2002.32698"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.G.01255"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.E.01149"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.D.01803"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.arth.2011.03.025"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0883-5403(93)90008-R"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1148\/radiographics.21.5.g01se191229"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11999-010-1245-4"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.F.00609"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.I.00400"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.10.3281-3290.1999"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.1747-1752.2000"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.5.2264-2267.2004"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00869-05"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.3928\/01477447-20110826-08"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.2106\/JBJS.L.00210"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.17.4.840-862.2004"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.2.335-351.1994"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.7.2155-2161.2002"}],"container-title":["The Scientific World Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2013\/950548.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2013\/950548.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1155\/2013\/950548","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,6]],"date-time":"2024-07-06T14:43:34Z","timestamp":1720277014000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1155\/2013\/950548"}},"subtitle":[],"editor":[{"given":"D.","family":"Sakai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]},{"given":"O.","family":"Wahlstrom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1155\/2013\/950548"],"URL":"https:\/\/doi.org\/10.1155\/2013\/950548","archive":["Portico"],"relation":{},"ISSN":["2356-6140","1537-744X"],"issn-type":[{"value":"2356-6140","type":"print"},{"value":"1537-744X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1]]},"assertion":[{"value":"2013-08-13","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"950548"}}