{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:23:41Z","timestamp":1791307421502,"version":"4.3.1"},"reference-count":33,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Deanship of Scientific Research at King Saud University","award":["RGP-VPP-038"],"award-info":[{"award-number":["RGP-VPP-038"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>This study was designed to investigate the prevalence of metallo-<jats:italic>\u03b2<\/jats:italic>-lactamases (MBL) and extended-spectrum<jats:italic>\u03b2<\/jats:italic>-lactamases (ESBL) in<jats:italic>P. aeruginosa<\/jats:italic>isolates collected from two different hospitals in Cairo, Egypt. Antibiotic susceptibility testing and phenotypic screening for ESBLs and MBLs were performed on 122<jats:italic>P. aeruginosa<\/jats:italic>isolates collected in the period from January 2011 to March 2012. MICs were determined. ESBLs and MBLs genes were sought by PCR. The resistant rate to imipenem was 39.34%. The resistance rates for<jats:italic>P. aeruginosa<\/jats:italic>to cefuroxime, cefoperazone, ceftazidime, aztreonam, and piperacillin\/tazobactam were 87.7%, 80.3%, 60.6%, 45.1%, and 25.4%, respectively. Out of 122<jats:italic>P. aeruginosa<\/jats:italic>, 27% and 7.4% were MBL and ESBL, respectively. The prevalence of<jats:italic>bla<\/jats:italic><jats:sub>VIM<jats:italic>-<\/jats:italic>2<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>OXA<jats:italic>-<\/jats:italic>10<\/jats:sub>-,<jats:italic>bla<\/jats:italic><jats:sub>VEB<jats:italic>-<\/jats:italic>1<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>NDM<\/jats:sub>-, and<jats:italic>bla<\/jats:italic><jats:sub>IMP<jats:italic>-<\/jats:italic>1<\/jats:sub>-like genes were found in 58.3%, 41.7%, 10.4%, 4.2%, and 2.1%, respectively. GIM-, SPM-, SIM-, and OXA-2-like genes were not detected in this study. OXA-10-like gene was concomitant with VIM-2 and\/or VEB. Twelve isolates harbored both OXA-10 and VIM-2; two isolates carried both OXA-10 and VEB. Only one strain contained OXA-10, VIM-2, and VEB. In conclusion,<jats:italic>bla<\/jats:italic><jats:sub>VIM<jats:italic>-<\/jats:italic>2<\/jats:sub>- and<jats:italic>bla<\/jats:italic><jats:sub>OXA<jats:italic>-<\/jats:italic>10<\/jats:sub>-like genes were the most prevalent genes in<jats:italic>P. aeruginosa<\/jats:italic>in Egypt. To our knowledge, this is the first report of<jats:italic>bla<\/jats:italic><jats:sub>VIM<jats:italic>-<\/jats:italic>2<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>IMP<jats:italic>-<\/jats:italic>1<\/jats:sub>,<jats:italic>bla<\/jats:italic><jats:sub>NDM<\/jats:sub>, and<jats:italic>bla<\/jats:italic><jats:sub>OXA<jats:italic>-<\/jats:italic>10<\/jats:sub>in<jats:italic>P. aeruginosa<\/jats:italic>in Egypt.<\/jats:p>","DOI":"10.1155\/2014\/101635","type":"journal-article","created":{"date-parts":[[2014,2,23]],"date-time":"2014-02-23T21:00:38Z","timestamp":1393189238000},"page":"1-8","source":"Crossref","is-referenced-by-count":65,"title":["Antimicrobial Resistance Pattern and Their Beta-Lactamase Encoding Genes among<i>Pseudomonas aeruginosa<\/i>Strains Isolated from Cancer Patients"],"prefix":"10.1155","volume":"2014","author":[{"given":"Mai M.","family":"Zafer","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Faculty of Pharmacy, Ahram Canadian University, Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed H.","family":"Al-Agamy","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics and Microbiology, College of Pharmacy, King Saud University, P.O. 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