{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T21:24:55Z","timestamp":1790803495569,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Disease Markers"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Background<\/jats:italic>. It is well known that diabetes mellitus may cause testicular damage. Vascular endothelial growth factor (VEGF) and nerve growth factor beta (NGF-<jats:italic>\u03b2<\/jats:italic>) are important neurotrophic factors for male reproductive system.<jats:italic>Objective<\/jats:italic>. We aimed to investigate the correlation between testicular damage and testicular VEGF and NGF-<jats:italic>\u03b2<\/jats:italic>levels in diabetic rats.<jats:italic>Methods<\/jats:italic>. Diabetes was induced by streptozotocin (STZ, 45\u2009mg\/kg\/i.p.) in adult rats. Five weeks later testicular tissue was removed; testicular VEGF and NGF-<jats:italic>\u03b2<\/jats:italic>levels were measured by ELISA. Testicular damage was detected by using hematoxylin and eosin staining and periodic acid-Schiff staining, and apoptosis was identified by terminal-deoxynucleotidyl-transferase-mediated dUTP nick end labeling (TUNEL). Seminiferous tubular sperm formation was evaluated using Johnsen\u2019s score.<jats:italic>Results<\/jats:italic>. In diabetic rats, seminiferous tubule diameter was found to be decreased; basement membrane was found to be thickened in seminiferous tubules and degenerated germ cells. Additionally, TUNEL-positive cells were increased in number of VEGF+ cells and levels of VEGF and NGF-<jats:italic>\u03b2<\/jats:italic>were decreased in diabetic testes. Correlation between VEGF and NGF-<jats:italic>\u03b2<\/jats:italic>levels was strong.<jats:italic>Conclusion<\/jats:italic>. These results suggest that the decrease of VEGF and NGF-<jats:italic>\u03b2<\/jats:italic>levels is associated with the increase of the apoptosis and testicular damage in diabetic rats. Testis VEGF and NGF-<jats:italic>\u03b2<\/jats:italic>levels could be potential novel biomarkers for diabetes induced testicular damage.<\/jats:p>","DOI":"10.1155\/2014\/108106","type":"journal-article","created":{"date-parts":[[2014,3,20]],"date-time":"2014-03-20T17:00:42Z","timestamp":1395334842000},"page":"1-7","source":"Crossref","is-referenced-by-count":36,"title":["Potential Novel Biomarkers for Diabetic Testicular Damage in Streptozotocin-Induced Diabetic Rats: Nerve Growth Factor Beta and Vascular Endothelial Growth Factor"],"prefix":"10.1155","volume":"2014","author":[{"given":"Ali R\u0131za","family":"Sisman","sequence":"first","affiliation":[{"name":"Department of Biochemistry, Dokuz Eylul University, 35340 Izmir, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muge","family":"Kiray","sequence":"additional","affiliation":[{"name":"Department of Physiology, Dokuz Eylul University, 35340 Izmir, Turkey"},{"name":"Division of Behavioral Physiology, 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