{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T18:52:48Z","timestamp":1760122368458,"version":"3.37.3"},"reference-count":43,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,10,2]],"date-time":"2014-10-02T00:00:00Z","timestamp":1412208000000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000005","name":"U.S. Department of Defense","doi-asserted-by":"publisher","award":["W911SR-11-C-0017"],"award-info":[{"award-number":["W911SR-11-C-0017"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["International Scholarly Research Notices"],"published-print":{"date-parts":[[2014,10,2]]},"abstract":"<jats:p>The etiologic agents for melioidosis and glanders,<jats:italic> Burkholderia mallei<\/jats:italic> and<jats:italic> Burkholderia pseudomallei<\/jats:italic> respectively, are genetically similar making identification and differentiation from other<jats:italic> Burkholderia<\/jats:italic> species and each other challenging. We used pyrosequencing to determine the presence or absence of an insertion sequence<jats:italic> IS<\/jats:italic>407A within the flagellin P (<jats:italic>fli<\/jats:italic>P) gene and to exploit the difference in orientation of this gene in the two species. Oligonucleotide primers were designed to selectively target the<jats:italic> IS<\/jats:italic>407A-<jats:italic>fli<\/jats:italic>P interface in<jats:italic> B. mallei<\/jats:italic> and the<jats:italic> fli<\/jats:italic>P gene specifically at the insertion point in<jats:italic> B. pseudomallei<\/jats:italic>. We then examined DNA from ten<jats:italic> B. mallei<\/jats:italic>, ten<jats:italic> B. pseudomallei,<\/jats:italic> 14<jats:italic> B. cepacia<\/jats:italic>, eight other<jats:italic> Burkholderia<\/jats:italic> spp., and 17 other bacteria. Resultant pyrograms encompassed the target sequence that contained either the<jats:italic> fli<\/jats:italic>P gene with the<jats:italic> IS<\/jats:italic>407A interruption or the fully intact<jats:italic> fli<\/jats:italic>P gene with 100% sensitivity and 100% specificity. These pyrosequencing assays based upon a single gene enable investigators to reliably identify the two species. The information obtained by these assays provides more knowledge of the genomic reduction that created the new species<jats:italic> B. mallei <\/jats:italic>from<jats:italic> B. pseudomallei<\/jats:italic> and may point to new targets that can be exploited in the future.<\/jats:p>","DOI":"10.1155\/2014\/109583","type":"journal-article","created":{"date-parts":[[2014,10,2]],"date-time":"2014-10-02T21:01:14Z","timestamp":1412283674000},"page":"1-10","source":"Crossref","is-referenced-by-count":9,"title":["The Identification and Differentiation between <i>Burkholderia mallei<\/i> and <i>Burkholderia pseudomallei<\/i> Using One Gene Pyrosequencing"],"prefix":"10.1155","volume":"2014","author":[{"given":"Damian H.","family":"Gilling","sequence":"first","affiliation":[{"name":"Center for Biological Defense, College of Public Health, University of South Florida, Tampa, FL 33612, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vicki 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