{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,8]],"date-time":"2025-09-08T06:08:12Z","timestamp":1757311692288,"version":"3.37.3"},"reference-count":20,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Fundo de Incentivo \u00e0 Pesquisa e Eventos do Hospital de Cl\u00ednicas de Porto Alegre","award":["09-270","10\/0026-1"],"award-info":[{"award-number":["09-270","10\/0026-1"]}]},{"DOI":"10.13039\/501100004263","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado do Rio Grande do Sul","doi-asserted-by":"publisher","award":["09-270","10\/0026-1"],"award-info":[{"award-number":["09-270","10\/0026-1"]}],"id":[{"id":"10.13039\/501100004263","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","award":["09-270","10\/0026-1"],"award-info":[{"award-number":["09-270","10\/0026-1"]}],"id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Modified Hodge Test (MHT) has been suggested as screening tests for carbapenemases, but concerns regarding its difficult interpretation and common false-positive results obtained in the presence of other<jats:italic>\u03b2<\/jats:italic>-lactamases have been noted. This study aimed to quantify the enhanced growth formed by the indicator strain and thus evaluate the performance of a quantitative interpretation of MHT for KPC screening. MHT was performed in 50 KPC-producing isolates and 334 non-carbapenemase-producing isolates, using ertapenem (ETP) and meropenem (MEM) as substrates. The size of enhanced growth of indicator strain was measured for each isolate tested and for the positive control used, and a ratio was calculated. Our results revealed 17 different ETP and MEM ratios, with distinct sensitivity (SN) and specificity (SP). Higher SN combined to higher SP was achieved when ETP and MEM ratios were 0.45, with a SN value of 96% for both substrates and SP values of 99.4% and 100% for ETP and MEM, respectively. The quantification with both substrates increased SP of the test for KPC detection. Considering that MHT is the unique phenotypic test that is referred to by CLSI, a more accurate approach for its interpretation could be applied to make it a more useful tool.<\/jats:p>","DOI":"10.1155\/2014\/139305","type":"journal-article","created":{"date-parts":[[2014,3,27]],"date-time":"2014-03-27T03:23:47Z","timestamp":1395890627000},"page":"1-6","source":"Crossref","is-referenced-by-count":7,"title":["Performance of Quantification of Modified Hodge Test: An Evaluation with<i>Klebsiella pneumoniae<\/i>Carbapenemase-Producing Enterobacteriaceae Isolates"],"prefix":"10.1155","volume":"2014","author":[{"given":"Vanessa Bley","family":"Ribeiro","sequence":"first","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Rio Grande do Sul (UFRGS), 2752 Ipiranga Avenue, 90160-093 Porto Alegre, RS, Brazil"},{"name":"Laborat\u00f3rio de Pesquisa em Resist\u00eancia Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Cl\u00ednicas de Porto Alegre (HCPA), 2350 Ramiro Barcelos Street, 90035-903 Porto Alegre, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriano Rostirolla","family":"Linhares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa em Resist\u00eancia Bacteriana (LABRESIS), Centro de Pesquisa Experimental, Hospital de Cl\u00ednicas de Porto Alegre (HCPA), 2350 Ramiro Barcelos Street, 90035-903 Porto Alegre, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexandre P.","family":"Zavascki","sequence":"additional","affiliation":[{"name":"Infectious Diseases Service, HCPA, 2350 Ramiro Barcelos Street, 90035-903 Porto Alegre, RS, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Afonso Luis","family":"Barth","sequence":"additional","affiliation":[{"name":"Faculdade de Farm\u00e1cia, Universidade Federal do Rio Grande do Sul (UFRGS), 2752 Ipiranga Avenue, 90160-093 Porto Alegre, RS, Brazil"},{"name":"Laborat\u00f3rio de Pesquisa 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