{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T14:28:16Z","timestamp":1785767296892,"version":"3.56.0"},"reference-count":49,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004482","name":"Kuwait University","doi-asserted-by":"publisher","award":["MR04\/09"],"award-info":[{"award-number":["MR04\/09"]}],"id":[{"id":"10.13039\/501100004482","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Diabetes Research"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Angiotensin-(1-7) [Ang-(1-7)] may have beneficial effects in diabetes mellitus-induced erectile dysfunction (DMIED) but its molecular actions in the diabetic corpus cavernosum (CC) are not known. We characterized the effects of diabetes and\/or chronic in vivo administration of Ang-(1-7) on vascular reactivity in the rat corpus cavernosum (CC) and on protein expression levels of potential downstream effectors of the renin-angiotensin-aldosterone system (RAAS) such as angiotensin-converting enzyme (ACE), ACE2, Rho kinases 1 and 2 (ROCK1 and ROCK2), and omega-hydroxylase, the cytochrome-P450 enzyme that metabolizes arachidonic acid to form the vasoconstrictor, 20-hydroxyeicosatetraenoic acid. Streptozotocin-treated rats were chronicically administered Ang-(1-7) with or without A779, a Mas receptor antagonist, during weeks 4 to 6 of diabetes. Ang-(1-7) reversed diabetes-induced abnormal reactivity to vasoactive agents (endothelin-1, phenylepherine, and carbachol) in the CC without correcting hyperglycemia. Six weeks of diabetes led to elevated ACE, ROCK1, ROCK 2, and omega-hydroxylase and a concomitant decrease in ACE2 protein expression levels that were normalized by Ang-(1-7) treatment but not upon coadministration of A779. These data are supportive of the notion that the beneficial effects of Ang-(1-7) in DMIED involve counterregulation of diabetes-induced changes in ACE, ACE2, Rho kinases, and omega-hydroxylase proteins in the diabetic CC via a Mas receptor-dependent mechanism.<\/jats:p>","DOI":"10.1155\/2014\/142154","type":"journal-article","created":{"date-parts":[[2014,9,17]],"date-time":"2014-09-17T01:42:15Z","timestamp":1410918135000},"page":"1-10","source":"Crossref","is-referenced-by-count":24,"title":["Chronic Treatment with Ang-(1-7) Reverses Abnormal Reactivity in the Corpus Cavernosum and Normalizes Diabetes-Induced Changes in the Protein Levels of ACE, ACE2, ROCK1, ROCK2 and Omega-Hydroxylase in a Rat Model of Type 1 Diabetes"],"prefix":"10.1155","volume":"2014","author":[{"given":"Mariam H. M.","family":"Yousif","sequence":"first","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Batoul","family":"Makki","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed Z.","family":"El-Hashim","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Therapeutics, Faculty of Pharmacy, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3177-946X","authenticated-orcid":true,"given":"Saghir","family":"Akhtar","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibrahim F.","family":"Benter","sequence":"additional","affiliation":[{"name":"Department of Pharmacology and Toxicology, Faculty of Medicine, Kuwait University, P.O. Box 24923, 13110 Safat, Kuwait"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.2174\/1573399809666131126151024"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1111\/jsm.12248"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1124\/pr.111.004515"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdiacomp.2010.03.003"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1038\/485S2a"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabres.2011.10.029"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1111\/eci.12117"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1046\/j.1464-410x.1999.00142.x"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2008.01168.x"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-008-0076-0"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.pbb.2013.11.024"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1111\/jsm.12262"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.02.046"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2007.05.008"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2008.01188.x"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1042\/CS20130344"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1530\/JOE-12-0341"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00372.2006"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2007.08.002"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-8673.2009.00426.x"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00173.2007"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0b013e3182a3718f"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0400520101"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.3109\/03602532.2012.754460"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0082482"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.45.120403.100045"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2007.014269"},{"issue":"1","key":"29","doi-asserted-by":"crossref","first-page":"388","DOI":"10.1016\/S0022-3565(24)37208-8","volume":"284","year":"1998","journal-title":"Journal of Pharmacology and Experimental Therapeutics"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0b013e31821324b6"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2012.01905.x"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1007\/s10571-012-9858-7"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.2011.01613.x"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2012.05.001"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0067813"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1007\/s00424-012-1120-0"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijir.3901534"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2009.01517.x"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)64901-2"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2008.02.090"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1124\/mol.105.017830"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.3109\/10409238.2013.786671"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.09.104"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2010.02147.x"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1111\/jsm.12134"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-8673.2009.00429.x"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1159\/000317521"},{"issue":"3","key":"48","doi-asserted-by":"crossref","first-page":"252","DOI":"10.1016\/j.phrs.2005.04.001","volume":"52","year":"2005","journal-title":"Pharmacological Research"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1111\/j.1474-8673.2005.00343.x"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2012.068551"}],"container-title":["Journal of Diabetes Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2014\/142154.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2014\/142154.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jdr\/2014\/142154.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,4]],"date-time":"2025-05-04T17:37:10Z","timestamp":1746380230000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/jdr\/2014\/142154\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":49,"alternative-id":["142154","142154"],"URL":"https:\/\/doi.org\/10.1155\/2014\/142154","relation":{},"ISSN":["2314-6745","2314-6753"],"issn-type":[{"value":"2314-6745","type":"print"},{"value":"2314-6753","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}