{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T18:38:27Z","timestamp":1789151907081,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003718","name":"Korean Institute of Oriental Medicine","doi-asserted-by":"crossref","award":["K14050"],"award-info":[{"award-number":["K14050"]}],"id":[{"id":"10.13039\/501100003718","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Analytical Methods in Chemistry"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>The<jats:italic>Acer tegmentosum<\/jats:italic>(3\u2009kg) was extracted using hot water, and the freeze-dried extract powder was partitioned successively using dichloromethane (DCM), ethyl acetate (EA), butyl alcohol (<jats:italic>n<\/jats:italic>-BuOH), and water. From the EA extract fraction (1.24\u2009g), five phenolic compounds were isolated by the silica gel, octadecyl silica gel, and Sephadex LH-20 column chromatography. Based on spectroscopic methods such as<jats:sup>1<\/jats:sup>H-NMR,<jats:sup>13<\/jats:sup>C-NMR, and LC\/MS the chemical structures of the compounds were confirmed as feniculin (<jats:bold>1<\/jats:bold>), avicularin (<jats:bold>2<\/jats:bold>), (+)-catechin (<jats:bold>3<\/jats:bold>), (\u2212)-epicatechin (<jats:bold>4<\/jats:bold>), and 6\u2032-<jats:italic>O<\/jats:italic>-galloyl salidroside (<jats:bold>5<\/jats:bold>). Moreover, a rapid on-line screening HPLC-ABTS<jats:sup>+<\/jats:sup>system for individual bioactivity of the EA-soluble fraction (five phenolic compounds) was developed. The results indicated that compounds<jats:bold>1<\/jats:bold>and<jats:bold>2<\/jats:bold>were first isolated from the<jats:italic>A. tegmentosum<\/jats:italic>. The anti-inflammatory activities and on-line screening HPLC-ABTS<jats:sup>+<\/jats:sup>assay method of these compounds in LPS-stimulated murine macrophages were rapid and efficient for the investigation of bioactivity of<jats:italic>A. tegmentosum<\/jats:italic>.<\/jats:p>","DOI":"10.1155\/2014\/150509","type":"journal-article","created":{"date-parts":[[2014,10,15]],"date-time":"2014-10-15T17:05:11Z","timestamp":1413392711000},"page":"1-15","source":"Crossref","is-referenced-by-count":17,"title":["Isolation and Bioactivity Analysis of Ethyl Acetate Extract from<i>Acer tegmentosum<\/i>Using In Vitro Assay and On-Line Screening HPLC-ABTS<sup>+<\/sup>System"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7800-5448","authenticated-orcid":true,"given":"Kwang Jin","family":"Lee","sequence":"first","affiliation":[{"name":"Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Na-Young","family":"Song","sequence":"additional","affiliation":[{"name":"Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"You Chang","family":"Oh","sequence":"additional","affiliation":[{"name":"Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Won-Kyung","family":"Cho","sequence":"additional","affiliation":[{"name":"Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin Yeul","family":"Ma","sequence":"additional","affiliation":[{"name":"Korean Institute of Oriental Medicine (KIOM), KM-Based Herbal Drug Development Group, 1672 Yuseongdae-ro, Yuseong-gu, Daejeon 305-811, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2004.08.007"},{"key":"2","first-page":"293","volume":"57","year":"2013","journal-title":"Yakhak Hoeji"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1016\/j.jep.2009.12.042"},{"key":"4","first-page":"149","volume":"49","year":"2006","journal-title":"Journal of the Korean Society for Applied Biological Chemistry"},{"issue":"1","key":"5","first-page":"67","volume":"38","year":"2007","journal-title":"Korean Journal of Pharmacognosy"},{"issue":"2","key":"6","first-page":"188","volume":"44","year":"2013","journal-title":"Korean Journal of 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