{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,15]],"date-time":"2026-03-15T11:13:44Z","timestamp":1773573224616,"version":"3.50.1"},"reference-count":47,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo","doi-asserted-by":"publisher","award":["2011A610036"],"award-info":[{"award-number":["2011A610036"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo","doi-asserted-by":"publisher","award":["2009C03013-3"],"award-info":[{"award-number":["2009C03013-3"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007834","name":"Natural Science Foundation of Ningbo","doi-asserted-by":"publisher","award":["2012C5017"],"award-info":[{"award-number":["2012C5017"]}],"id":[{"id":"10.13039\/100007834","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008990","name":"Science and Technology Department of Zhejiang Province","doi-asserted-by":"crossref","award":["2011A610036"],"award-info":[{"award-number":["2011A610036"]}],"id":[{"id":"10.13039\/501100008990","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008990","name":"Science and Technology Department of Zhejiang Province","doi-asserted-by":"crossref","award":["2009C03013-3"],"award-info":[{"award-number":["2009C03013-3"]}],"id":[{"id":"10.13039\/501100008990","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008990","name":"Science and Technology Department of Zhejiang Province","doi-asserted-by":"crossref","award":["2012C5017"],"award-info":[{"award-number":["2012C5017"]}],"id":[{"id":"10.13039\/501100008990","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Advanced key Scientific and Technological Programs of Ningbo","award":["2011A610036"],"award-info":[{"award-number":["2011A610036"]}]},{"name":"Advanced key Scientific and Technological Programs of Ningbo","award":["2009C03013-3"],"award-info":[{"award-number":["2009C03013-3"]}]},{"name":"Advanced key Scientific and Technological Programs of Ningbo","award":["2012C5017"],"award-info":[{"award-number":["2012C5017"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Objective<\/jats:italic>. Rheumatic heart disease (RHD) is a serious autoimmune heart disease. The present study was aimed at identifying the differentially expressed proteins between patients with RHD and controls with mitral valve prolapse.<jats:italic>Methods<\/jats:italic>. Nine patients with RHD and nine controls with mitral valve prolapsed were enrolled for this study. Two-dimensional difference in-gel electrophoresis (2D-DIGE) and matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) were performed.<jats:italic>Results<\/jats:italic>. A total of 39 protein spots with differential expressions were identified between the two groups (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.05<\/mml:mn><\/mml:math>, Average Ratio &gt; 1.2 or Average Ratio &lt; \u22121.2) and four upregulated proteins (including heat shock protein 60 (HSP 60), desmin, PDZ and LIM domain protein 1, and proteasome subunit alpha type-1) and three downregulated proteins (including tropomyosin alpha-1 chain, malate dehydrogenase, and chaperone activity of bc1 complex homolog) were determined.<jats:italic>Conclusion<\/jats:italic>. These seven proteins, especially HSP 60, may serve as potential biomarkers for the diagnosis of RHD and provide evidence to explain the mechanisms of this complex disease in the future.<\/jats:p>","DOI":"10.1155\/2014\/151726","type":"journal-article","created":{"date-parts":[[2014,3,12]],"date-time":"2014-03-12T21:01:10Z","timestamp":1394658070000},"page":"1-9","source":"Crossref","is-referenced-by-count":5,"title":["Comparison of the Ventricle Muscle Proteome between Patients with Rheumatic Heart Disease and Controls with Mitral Valve Prolapse: HSP 60 May Be a Specific Protein in RHD"],"prefix":"10.1155","volume":"2014","author":[{"given":"Dawei","family":"Zheng","sequence":"first","affiliation":[{"name":"Department of Cardiothoracic Surgery, The Affiliated Hospital, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"},{"name":"Department of Cardiothoracic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Limin","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The Affiliated Hospital, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lebo","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The Affiliated Hospital, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zishan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The Affiliated Hospital, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guofeng","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The Affiliated Hospital, Ningbo Medical Centre Lihuili Hospital, Ningbo University, Ningbo, Zhejiang 315041, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiming","family":"Ni","sequence":"additional","affiliation":[{"name":"Department of Cardiothoracic Surgery, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1007\/s12098-012-0853-2"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/j.micinf.2012.07.003"},{"key":"3","year":"2007"},{"key":"4","first-page":"13","volume":"60","year":"2012","journal-title":"Journal of the Association of Physicians of India"},{"issue":"3","key":"5","first-page":"135","volume":"19","year":"2008","journal-title":"Cardiovascular Journal of Africa"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2010.10.004"},{"issue":"10","key":"7","doi-asserted-by":"crossref","first-page":"1216","DOI":"10.1002\/1615-9861(200110)1:10<1216::AID-PROT1216>3.0.CO;2-P","volume":"1","year":"2001","journal-title":"Proteomics"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2008.04.023"},{"issue":"1","key":"9","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1111\/j.1582-4934.2005.tb00336.x","volume":"9","year":"2005","journal-title":"Journal of Cellular and Molecular Medicine"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.209643"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00835.2012"},{"issue":"6","key":"12","doi-asserted-by":"crossref","first-page":"3212","DOI":"10.4049\/jimmunol.162.6.3212","volume":"162","year":"1999","journal-title":"Journal of Immunology"},{"issue":"1","key":"13","doi-asserted-by":"crossref","first-page":"13","DOI":"10.4049\/jimmunol.164.1.13","volume":"164","year":"2000","journal-title":"Journal of Immunology"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvt047"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00740.2007"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1006\/jmcc.1998.0646"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1385\/1-59745-214-9:33"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.2000.01283.x"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2009.11.009"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1097\/SHK.0b013e3182094a0b"},{"issue":"3","key":"21","doi-asserted-by":"crossref","first-page":"H919","DOI":"10.1152\/ajpheart.00851.2002","volume":"284","year":"2003","journal-title":"American Journal of Physiology\u2014Heart and Circulatory Physiology"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2008.09.021"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1007\/s004390050233"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00601.2011"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejheart.2003.07.010"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2796.2012.02524.x"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2008.09.026"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1499"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200707045"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2008.05621.x"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjp038"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.139.2.507"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1038\/87920"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-005-3604-y"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.5.1682-1687.2001"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1007\/s004290050050"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1023\/A:1010303732441"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/S1937-6448(10)81003-2"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2009.10.003"},{"key":"40","first-page":"257","volume":"21","year":"2002","journal-title":"General Physiology and Biophysics"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-012-9397-0"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2009.09.012"},{"key":"43","first-page":"37","volume":"15","year":"1994","journal-title":"European Heart Journal"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2007.12.022"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1136\/jnnp-2011-301258"},{"issue":"5","key":"46","first-page":"1246","volume":"62","year":"2002","journal-title":"Cancer Research"},{"issue":"3","key":"47","first-page":"193","volume":"17","year":"1998","journal-title":"General Physiology and Biophysics"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/151726.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/151726.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/151726.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,7,11]],"date-time":"2023-07-11T04:44:25Z","timestamp":1689050665000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2014\/151726\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":47,"alternative-id":["151726","151726"],"URL":"https:\/\/doi.org\/10.1155\/2014\/151726","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}