{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T13:24:05Z","timestamp":1791725045631,"version":"4.3.4"},"reference-count":69,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003711","name":"Ministry of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003711","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004809","name":"Financiadora de Estudos e Projetos","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005287","name":"Funda\u00e7\u00e3o de Tecnologia do Acre","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005287","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de N\u00edvel Superior","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Rede de Biodiversidade e Biotecnologia da Amaz\u00f4nia Legal"},{"DOI":"10.13039\/501100005292","name":"Instituto Nacional para Pesquisa Translacional em Sa\u00fade e Ambiente na Regi\u00e3o Amaz\u00f4nica","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005292","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004933","name":"Instituto Nacional para Pesquisa em Toxinas","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004933","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Secretary of Development of Rondonia State"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Bothrops mattogrossensis<\/jats:italic>snake is widely distributed throughout eastern South America and is responsible for snakebites in this region. This paper reports the purification and biochemical characterization of three new phospholipases A<jats:sub>2<\/jats:sub>(PLA<jats:sub>2<\/jats:sub>s), one of which is presumably an enzymatically active Asp49 and two are very likely enzymatically inactive Lys49 PLA<jats:sub>2<\/jats:sub>homologues. The purification was obtained after two chromatographic steps on ion exchange and reverse phase column. The 2D SDS-PAGE analysis revealed that the proteins have pI values around 10, are each made of a single chain, and have molecular masses near 13 kDa, which was confirmed by MALDI-TOF mass spectrometry. The N-terminal similarity analysis of the sequences showed that the proteins are highly homologous with other Lys49 and Asp49 PLA<jats:sub>2<\/jats:sub>s from<jats:italic>Bothrops<\/jats:italic>species. The PLA<jats:sub>2<\/jats:sub>s isolated were named BmatTX-I (Lys49 PLA<jats:sub>2<\/jats:sub>-like), BmatTX-II (Lys49 PLA<jats:sub>2<\/jats:sub>-like), and BmatTX-III (Asp49 PLA<jats:sub>2<\/jats:sub>). The PLA<jats:sub>2<\/jats:sub>s induced cytokine release from mouse neutrophils and showed cytotoxicity towards JURKAT (leukemia T) and SK-BR-3 (breast adenocarcinoma) cell lines and promastigote forms of<jats:italic>Leishmania amazonensis<\/jats:italic>. The structural and functional elucidation of snake venoms components may contribute to a better understanding of the mechanism of action of these proteins during envenomation and their potential pharmacological and therapeutic applications.<\/jats:p>","DOI":"10.1155\/2014\/195356","type":"journal-article","created":{"date-parts":[[2014,3,3]],"date-time":"2014-03-03T21:00:36Z","timestamp":1393880436000},"page":"1-13","source":"Crossref","is-referenced-by-count":40,"title":["Purification and Biochemical Characterization of Three Myotoxins from<i>Bothrops mattogrossensis<\/i>Snake Venom with Toxicity against<i>Leishmania<\/i>and Tumor Cells"],"prefix":"10.1155","volume":"2014","author":[{"given":"Andr\u00e9a A. de","family":"Moura","sequence":"first","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas \u00e0 Saude, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, N\u00facleo de Sa\u00fade, Universidade Federal de Rond\u00f4nia, UNIR, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anderson M.","family":"Kayano","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas \u00e0 Saude, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, N\u00facleo de Sa\u00fade, Universidade Federal de Rond\u00f4nia, UNIR, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George A.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas \u00e0 Saude, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, N\u00facleo de Sa\u00fade, Universidade Federal de Rond\u00f4nia, UNIR, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sulamita S.","family":"Set\u00fabal","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o G.","family":"Ribeiro","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas \u00e0 Saude, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, N\u00facleo de Sa\u00fade, Universidade Federal de Rond\u00f4nia, UNIR, 76812\u2013245 Porto Velho, RO, Brazil"},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neuza B.","family":"Barros","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Nicolete","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura A.","family":"Moura","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, UFF, 24020-141 Niter\u00f3i, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre L.","family":"Fuly","sequence":"additional","affiliation":[{"name":"Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, UFF, 24020-141 Niter\u00f3i, RJ, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Auro","family":"Nomizo","sequence":"additional","affiliation":[{"name":"Faculdade de Ci\u00eancias Farmac\u00eauticas, FCFRP, Universidade de S\u00e3o Paulo, USP, 14040-903 Ribeir\u00e3o Preto, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saulo L.","family":"da Silva","sequence":"additional","affiliation":[{"name":"Universidade Federal de S\u00e3o Jo\u00e3o del Rei, UFSJ, 36420-000 Ouro Branco, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carla F. C.","family":"Fernandes","sequence":"additional","affiliation":[{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juliana P.","family":"Zuliani","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas \u00e0 Saude, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, N\u00facleo de Sa\u00fade, Universidade Federal de Rond\u00f4nia, UNIR, 76812\u2013245 Porto Velho, RO, Brazil"},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rodrigo G.","family":"St\u00e1beli","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas \u00e0 Saude, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, N\u00facleo de Sa\u00fade, Universidade Federal de Rond\u00f4nia, UNIR, 76812\u2013245 Porto Velho, RO, Brazil"},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andreimar M.","family":"Soares","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas \u00e0 Saude, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, N\u00facleo de Sa\u00fade, Universidade Federal de Rond\u00f4nia, UNIR, 76812\u2013245 Porto Velho, RO, Brazil"},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonardo A.","family":"Calderon","sequence":"additional","affiliation":[{"name":"Centro de Estudos de Biomol\u00e9culas Aplicadas \u00e0 Saude, CEBio, Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia e Departamento de Medicina, N\u00facleo de Sa\u00fade, Universidade Federal de Rond\u00f4nia, UNIR, 76812\u2013245 Porto Velho, RO, Brazil"},{"name":"Funda\u00e7\u00e3o Oswaldo Cruz, Fiocruz Rond\u00f4nia, 76812\u2013245 Porto Velho, RO, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2008.12.014"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1002\/jms.1242"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2011.08115.x"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.5772\/2707"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.11.004"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2012.12.020"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1134\/S0006297913020089"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2007.08.007"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/789689"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.11606\/issn.2316-9079.v7i1p45-90"},{"key":"12","year":"2004"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.3891\/acta.chem.scand.04-0283"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(96)00057-8"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(02)00873-0"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(84)90393-X"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(89)90096-2"},{"key":"20","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1016\/S0021-9258(17)41059-3","volume":"171","year":"1947","journal-title":"The Journal of Biological Chemistry"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpc.2005.11.020"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64014-X"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1023\/B:JOPC.0000027852.92208.60"},{"issue":"5","key":"24","doi-asserted-by":"crossref","first-page":"1576","DOI":"10.4049\/jimmunol.141.5.1576","volume":"141","year":"1988","journal-title":"The Journal of Immunology"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(83)90303-4"},{"key":"26","first-page":"28","volume":"3","year":"2012","journal-title":"Journal of Venom Research"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(95)00085-Z"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)01163-9"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-4838(99)00268-X"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.2174\/1385272043369610"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.06.008"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2008.08.020"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1999.1492"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.2000.2244"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1007\/s00204-011-0665-6"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/S0300-9084(00)01150-0"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(02)01210-8"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2010.02.031"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2009.01.003"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2009.02.001"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(88)90244-9"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2009.07.031"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/S0041-0101(98)00188-3"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1016\/S1357-2725(03)00237-1"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(90)90114-M"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(95)00012-B"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(90)90062-C"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1021\/cr200085w"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2009.10.025"},{"key":"52","doi-asserted-by":"publisher","DOI":"10.1016\/S1388-1981(00)00110-4"},{"issue":"4","key":"53","first-page":"647","volume":"58","year":"2011","journal-title":"Acta Chimica Slovenica"},{"key":"54","doi-asserted-by":"publisher","DOI":"10.1016\/0041-0101(84)90144-2"},{"key":"55","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2009.08.009"},{"key":"56","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2003.11.006"},{"key":"57","doi-asserted-by":"publisher","DOI":"10.1080\/0962935021000051548"},{"key":"58","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2004.10.005"},{"key":"59","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02762005000900031"},{"key":"60","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2006.11.006"},{"key":"61","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpba.2012.04.008"},{"key":"62","doi-asserted-by":"publisher","DOI":"10.1080\/09629350220131980"},{"key":"63","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0510263"},{"key":"64","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2005.06.017"},{"key":"65","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2005.08.003"},{"key":"66","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(01)00841-3"},{"key":"67","doi-asserted-by":"publisher","DOI":"10.1016\/j.toxicon.2009.05.040"},{"key":"68","doi-asserted-by":"publisher","DOI":"10.1016\/j.tiv.2007.04.010"},{"issue":"3","key":"69","doi-asserted-by":"crossref","first-page":"793","DOI":"10.1016\/S0022-3565(24)39138-4","volume":"294","year":"2000","journal-title":"The Journal of Pharmacology and Experimental Therapeutics"},{"issue":"10","key":"70","doi-asserted-by":"crossref","first-page":"1678","DOI":"10.1016\/S0022-2275(20)32223-9","volume":"42","year":"2001","journal-title":"Journal of Lipid Research"},{"key":"71","doi-asserted-by":"publisher","DOI":"10.1046\/j.1432-1327.1998.2530452.x"},{"key":"72","doi-asserted-by":"publisher","DOI":"10.2174\/138161210793292456"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/195356.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/195356.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/195356.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T23:49:47Z","timestamp":1746143387000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2014\/195356\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":69,"alternative-id":["195356","195356"],"URL":"https:\/\/doi.org\/10.1155\/2014\/195356","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}