{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:23:45Z","timestamp":1740173025748,"version":"3.37.3"},"reference-count":32,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Chinese National Mega Science & Technology Program on Infectious Diseases","award":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"],"award-info":[{"award-number":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"],"award-info":[{"award-number":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"],"award-info":[{"award-number":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"],"award-info":[{"award-number":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"],"award-info":[{"award-number":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100013105","name":"Shanghai Rising-Star Program","doi-asserted-by":"crossref","award":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"],"award-info":[{"award-number":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"]}],"id":[{"id":"10.13039\/501100013105","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shanghai Health Bureau","award":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"],"award-info":[{"award-number":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"]}]},{"name":"Shanghai Science and Technology Commission","award":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"],"award-info":[{"award-number":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"]}]},{"name":"Shanghai Science and Technology Commission","award":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"],"award-info":[{"award-number":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"]}]},{"name":"Shanghai Natural Science Fund for Youth Scholars","award":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"],"award-info":[{"award-number":["2013ZX10003007-003","81273328","31170876","81301407","81101213","12QH1401900","20114013","10411962700","134119a5200","12ZR1448200"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>One approach for improving BCG efficacy is to utilize BCG as vehicle to develop recombinant BCG (rBCG) strains overexpressing<jats:italic>Mycobacterium tuberculosis (M. tb)<\/jats:italic>antigens. Also expression level of a candidate antigen should impact the final T cell responses conferred by rBCG. In this study, based on our previously constructed differential expression system, we developed two rBCG strains overexpressing<jats:italic>M. tb<\/jats:italic>chimeric antigen Ag856A2 (coding a recombinant<jats:italic>ag85a<\/jats:italic>with 2 copies of<jats:italic>esat-6<\/jats:italic>inserted at<jats:italic>Acc<\/jats:italic>I site of<jats:italic>ag85a<\/jats:italic>) at differential levels under the control of the subtly modified<jats:italic>furA<\/jats:italic>promoters. These two rBCG strains were used to vaccinate C57BL\/6 mice and exploit dose of incorporated antigen in rBCG to optimize immune response and protective efficiency against<jats:italic>M. tb<\/jats:italic>challenge in mouse model. The results showed that rBCG strains overexpressing Ag856A2 at differential levels induced different antigen-specific IFN-<jats:italic>\u03b3<\/jats:italic>production and comparable number of<jats:italic>M. tb<\/jats:italic>-specific CD4 T cells expressing IL-2.<jats:italic>M. tb<\/jats:italic>challenge experiment showed that rBCG strains afforded enhanced but comparable immune protection characterized by reduced bacillary load, lung pathology, and inflammation. These results suggested that the dose of antigens incorporated in rBCG can impact T cell immune responses but imposed no significantly differential protective efficacies.<\/jats:p>","DOI":"10.1155\/2014\/196124","type":"journal-article","created":{"date-parts":[[2014,7,23]],"date-time":"2014-07-23T22:29:55Z","timestamp":1406154595000},"page":"1-9","source":"Crossref","is-referenced-by-count":2,"title":["Dose of Incorporated Immunodominant Antigen in Recombinant BCG Impacts Modestly on Th1 Immune Response and Protective Efficiency against<i>Mycobacterium tuberculosis<\/i>in Mice"],"prefix":"10.1155","volume":"2014","author":[{"given":"Hui","family":"Ma","sequence":"first","affiliation":[{"name":"Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9024-795X","authenticated-orcid":true,"given":"Kang","family":"Wu","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fang","family":"Liu","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua","family":"Yang","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han","family":"Kang","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China"},{"name":"Key Laboratory of Medical Molecular Virology of MOE\/MOH, Shanghai Medical College, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning-Ning","family":"Chen","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Yuan","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen-Jiang","family":"Zhou","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6654-3559","authenticated-orcid":true,"given":"Xiao-Yong","family":"Fan","sequence":"additional","affiliation":[{"name":"Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai 201508, China"},{"name":"Key Laboratory of Medical Molecular Virology of MOE\/MOH, Shanghai Medical College, Fudan University, Shanghai 200032, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1086\/430786"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra021964"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24617"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/j.plasmid.2008.09.002"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2458-10-394"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60410-2"},{"issue":"1","key":"7","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1007\/BF02113516","volume":"25","year":"1997","journal-title":"Infection"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(00)88986-6"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1038\/nm859"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.250480397"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2012.03.004"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002607"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2478(03)00043-9"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005930"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(02)00289-X"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0029732"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2006.25.25"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.08.020"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003869"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4906(01)01865-8"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3083.2003.01321.x"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0023360"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1038\/nri2274"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1038\/nm.2285"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.173.9.5372"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/745483"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1054\/tuld.1998.0203"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.3.347"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/1074-7613(95)90001-2"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030186"},{"issue":"4","key":"31","first-page":"213","volume":"120","year":"2004","journal-title":"Indian Journal of Medical Research"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2008.00702.x"}],"container-title":["Journal of Immunology Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2014\/196124.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2014\/196124.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/jir\/2014\/196124.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T03:05:29Z","timestamp":1589252729000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/jir\/2014\/196124\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":32,"alternative-id":["196124","196124"],"URL":"https:\/\/doi.org\/10.1155\/2014\/196124","relation":{},"ISSN":["2314-8861","2314-7156"],"issn-type":[{"type":"print","value":"2314-8861"},{"type":"electronic","value":"2314-7156"}],"subject":[],"published":{"date-parts":[[2014]]}}}