{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,11]],"date-time":"2024-08-11T21:08:30Z","timestamp":1723410510775},"reference-count":38,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Aim.<\/jats:italic>To assess the efficiency of phosphorothioate antisense oligodeoxynucleotide 1 (S-ODN1) on HCV translation inhibition in PBMC compared to hepatoma cells<jats:italic>in vitro<\/jats:italic>for the first time.<jats:italic>Materials and Methods.<\/jats:italic>The study included 34 treatment naive HCV patients. IRES domain III and IV sequence variations were tested in 45 clones from 9 HCV patients. PBMC of HCV positive patients were subjected to S-ODN<jats:italic>in vitro<\/jats:italic>. Concomitantly HepG2 cells infected by the same patient\u2019s serum were also treated with S-ODN1 for 24 and 48 hours. Cellular RNA was tested for HCV plus and minus strands by reverse transcription polymerase chain reaction (RT-PCR).<jats:italic>Results.<\/jats:italic>Sequence variations were seen in HCV IRES domain III only while domain IV was conserved among all the tested patient\u2019s clones. S-ODN1 successfully inhibited HCV translation in HepG2 cells, while in PBMC inhibition was partial.<jats:italic>Conclusion.<\/jats:italic>HCV IRES domain IV is more conserved than domain IIId in genotype 4 HCV patients. S-ODN against HCV IRES domain IV was not efficient to inhibit HCV translation in PBMC under the study conditions. Further studies testing other S-ODN targeting other HCV IRES domains in PBMC should be done.<\/jats:p>","DOI":"10.1155\/2014\/196712","type":"journal-article","created":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T21:03:10Z","timestamp":1401656590000},"page":"1-7","source":"Crossref","is-referenced-by-count":2,"title":["<i>In Vitro<\/i>Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells"],"prefix":"10.1155","volume":"2014","author":[{"given":"Samar Samir","family":"Youssef","sequence":"first","affiliation":[{"name":"Microbial Biotechnology Department, National Research Center, Cairo 12311, Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed Mohamed","family":"Fahmy","sequence":"additional","affiliation":[{"name":"Reproductive Health and Family Planning Department, National Research Center, Cairo, Egypt"},{"name":"INRS-Institut Armand Frappier, Laval, QC, Canada H7V 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