{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T16:38:46Z","timestamp":1790786326711,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>The haemolytic uraemic syndrome (HUS) is the most frequent cause of acute renal failure in childhood. We investigated L-arginine\/NO pathway in 12 children with typical HUS and 12 age-matched healthy control subjects. Nitrite and nitrate, the major NO metabolites in plasma and urine, asymmetric dimethylarginine (ADMA) in plasma and urine, and dimethylamine (DMA) in urine were determined by GC-MS and GC-MS\/MS techniques. Urinary measurements were corrected for creatinine excretion. Plasma nitrate was significantly higher in HUS patients compared to healthy controls<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mfenced separators=\"|\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.021<\/mml:mn><\/mml:mrow><\/mml:mfenced><\/mml:mrow><\/mml:math>, whereas urine nitrate was borderline lower in HUS patients compared to healthy controls<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:mfenced separators=\"|\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.24<\/mml:mn><\/mml:mrow><\/mml:mfenced><\/mml:mrow><\/mml:math>. ADMA plasma concentrations were insignificantly lower, but urine ADMA levels were significantly lower in the HUS patients<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:mfenced separators=\"|\"><mml:mrow><mml:mi>P<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.019<\/mml:mn><\/mml:mrow><\/mml:mfenced><\/mml:mrow><\/mml:math>. Urinary DMA was not significantly elevated. In HUS patients, nitrate<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mrow><mml:mfenced separators=\"|\"><mml:mrow><mml:mi>R<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.91<\/mml:mn><\/mml:mrow><\/mml:mfenced><\/mml:mrow><\/mml:math>but not nitrite, L-arginine, or ADMA concentrations in plasma correlated with free haemoglobin concentration. Our results suggest that both NO production and ADMA synthesis are decreased in children with typical HUS. We hypothesize that in the circulation of children with HUS a vicious circle between the L-arginine\/NO pathway and free haemoglobin-mediated oxidative stress exists. Disruption of this vicious circle by drugs that release NO and\/or sulphydryl groups-containing drugs may offer new therapeutic options in HUS.<\/jats:p>","DOI":"10.1155\/2014\/203512","type":"journal-article","created":{"date-parts":[[2014,3,17]],"date-time":"2014-03-17T21:01:17Z","timestamp":1395090077000},"page":"1-9","source":"Crossref","is-referenced-by-count":10,"title":["L-Arginine\/NO Pathway Is Altered in Children with Haemolytic-Uraemic Syndrome (HUS)"],"prefix":"10.1155","volume":"2014","author":[{"given":"Nele Kirsten","family":"Kanzelmeyer","sequence":"first","affiliation":[{"name":"Department of Paediatric Kidney, Liver and Metabolic Diseases, Hannover Medical School, Carl-Neuberg-Stra\u00dfe 1, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Pape","sequence":"additional","affiliation":[{"name":"Department of Paediatric Kidney, Liver and Metabolic Diseases, Hannover Medical School, Carl-Neuberg-Stra\u00dfe 1, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristine","family":"Chobanyan-J\u00fcrgens","sequence":"additional","affiliation":[{"name":"Department of Paediatric Kidney, Liver and Metabolic Diseases, Hannover Medical School, Carl-Neuberg-Stra\u00dfe 1, 30625 Hannover, Germany"},{"name":"Institute of Clinical Pharmacology, Hannover Medical School, Carl-Neuberg-Stra\u00dfe 1, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dimitrios","family":"Tsikas","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Hannover Medical School, Carl-Neuberg-Stra\u00dfe 1, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4333-3839","authenticated-orcid":true,"given":"Hans","family":"Hartmann","sequence":"additional","affiliation":[{"name":"Department of Paediatric Kidney, Liver and Metabolic Diseases, Hannover Medical School, Carl-Neuberg-Stra\u00dfe 1, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne-Jule","family":"Fuchs","sequence":"additional","affiliation":[{"name":"Department of Paediatrics, University of Mainz, Langenbeckstra\u00dfe 1, 55131 Mainz, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernhard","family":"Vaske","sequence":"additional","affiliation":[{"name":"Institute of Biometry, Hannover Medical School, Carl-Neuberg-Stra\u00dfe 1, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anibh Martin","family":"Das","sequence":"additional","affiliation":[{"name":"Department of Paediatric Kidney, Liver and Metabolic Diseases, Hannover Medical School, Carl-Neuberg-Stra\u00dfe 1, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marion","family":"Haubitz","sequence":"additional","affiliation":[{"name":"Department of Medicine III, Klinikum Fulda, Pacelliallee 4, 36043 Fulda, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jens","family":"Jordan","sequence":"additional","affiliation":[{"name":"Institute of Clinical Pharmacology, Hannover Medical School, Carl-Neuberg-Stra\u00dfe 1, 30625 Hannover, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"L\u00fccke","sequence":"additional","affiliation":[{"name":"Department of Neuropaediatrics, University Children\u2019s Hospital, Ruhr University Bochum, Gudrunstra\u00dfe 56, 44791 Bochum, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1007\/s00467-006-0232-1"},{"issue":"6","key":"2","first-page":"991","volume":"74","year":"2006","journal-title":"The American Family Physician"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis531"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1086\/341940"},{"issue":"9","key":"5","doi-asserted-by":"crossref","first-page":"1102","DOI":"10.1016\/S0046-8177(88)80093-5","volume":"19","year":"1988","journal-title":"Human Pathology"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1007\/s004670050337"},{"issue":"3","key":"7","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1055\/s-2007-996101","volume":"23","year":"1997","journal-title":"Seminars in Thrombosis and Hemostasis"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1001\/jama.290.10.1360"},{"key":"9","first-page":"165","volume":"73","year":"2003","journal-title":"Methods in Molecular Medicine"},{"issue":"9","key":"10","doi-asserted-by":"crossref","first-page":"3375","DOI":"10.1073\/pnas.86.9.3375","volume":"86","year":"1989","journal-title":"Proceedings of the National Academy of Sciences of the United States of America"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.med.48.1.489"},{"key":"12","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1146\/annurev.pa.30.040190.002535","volume":"30","year":"1990","journal-title":"Annual Review of Pharmacology and Toxicology"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-005-0020-z"},{"issue":"17","key":"14","doi-asserted-by":"crossref","first-page":"12231","DOI":"10.1016\/S0021-9258(18)31375-9","volume":"268","year":"1993","journal-title":"The Journal of Biological Chemistry"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.0000081742.92006.59"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1007\/s00228-005-0013-y"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2001.07819.x"},{"issue":"5","key":"18","doi-asserted-by":"crossref","first-page":"477","DOI":"10.3109\/00365599709030646","volume":"31","year":"1997","journal-title":"Scandinavian Journal of Urology and Nephrology"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2007.300"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfm761"},{"issue":"3","key":"21","first-page":"137","volume":"47","year":"1997","journal-title":"Acta Physiologica, Pharmacologica et Therapeutica Latinoamericana"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfq511"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1111\/cei.12124"},{"issue":"1","key":"24","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1038\/sj.bmt.1702098","volume":"25","year":"2000","journal-title":"Bone Marrow Transplantation"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2009.09.001"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2003.09.001"},{"issue":"17","key":"27","doi-asserted-by":"crossref","first-page":"4064","DOI":"10.1021\/ac9913255","volume":"72","year":"2000","journal-title":"Analytical Chemistry"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2010.04.025"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.jchromb.2006.09.015"},{"key":"30","doi-asserted-by":"publisher","DOI":"10.1111\/j.1538-7836.2006.01794.x"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1159\/000110092"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2000.917307.x"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1055\/s-2006-954584"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2002.00554.x"},{"issue":"10","key":"35","doi-asserted-by":"crossref","first-page":"2088","DOI":"10.1681\/ASN.V12102088","volume":"12","year":"2001","journal-title":"Journal of the American Society of Nephrology"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1620\/tjem.228.247"},{"issue":"3","key":"37","doi-asserted-by":"crossref","first-page":"507","DOI":"10.1111\/j.1476-5381.1992.tb14365.x","volume":"106","year":"1992","journal-title":"The British Journal of Pharmacology"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1038\/nm799"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1038\/nm954"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409876102"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.22195"},{"issue":"4","key":"42","doi-asserted-by":"crossref","first-page":"284","DOI":"10.2174\/187152910793743878","volume":"10","year":"2010","journal-title":"Cardiovascular and Hematological Disorders\u2014Drug Targets"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2009.10.002"},{"issue":"4490","key":"44","doi-asserted-by":"crossref","first-page":"58","DOI":"10.1126\/science.7209517","volume":"212","year":"1981","journal-title":"Science"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1016\/j.niox.2013.03.002"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-200302000-00018"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1002\/mas.21396"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2012.03.020"},{"key":"49","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2010.11.003"},{"key":"50","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2012.09.010"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2014\/203512.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2014\/203512.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2014\/203512.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,30]],"date-time":"2022-03-30T02:04:50Z","timestamp":1648605890000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/omcl\/2014\/203512\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":50,"alternative-id":["203512","203512"],"URL":"https:\/\/doi.org\/10.1155\/2014\/203512","relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}