{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,8,6]],"date-time":"2024-08-06T05:22:21Z","timestamp":1722921741704},"reference-count":38,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>The present study describes the beneficial effects of potential probiotic<jats:italic>E. coli<\/jats:italic>16 (pUC8:16<jats:italic>gfp<\/jats:italic>) expressing<jats:italic>Vitreoscilla<\/jats:italic>hemoglobin (<jats:italic>vgb<\/jats:italic>) gene, associated with bacterial respiration under microaerobic condition, on gastrointestinal (GI) colonization and its antioxidant activity on carbon tetrachloride (CCl<jats:sub>4<\/jats:sub>) induced toxicity in Charles Foster rats.<jats:italic>In vitro<\/jats:italic>, catalase activity in<jats:italic>E. coli<\/jats:italic>16 (pUC8:16<jats:italic>gfp<\/jats:italic>) was 1.8 times higher compared to<jats:italic>E. coli<\/jats:italic>16 (pUC-<jats:italic>gfp<\/jats:italic>) control.<jats:italic>In vivo<\/jats:italic>,<jats:italic>E. coli<\/jats:italic>16 (pUC8:16<jats:italic>gfp<\/jats:italic>) not only was recovered in the fecal matter after 70 days of oral administration but also retained antibacterial activities, whereas<jats:italic>E. coli<\/jats:italic>16 (pUC-<jats:italic>gfp<\/jats:italic>) was not detected. Oral administration of 200 and 500\u2009<jats:italic>\u03bc<\/jats:italic>L\/kg body weight of CCl<jats:sub>4<\/jats:sub>to rats at weekly interval resulted in elevated serum glutamyl pyruvate transaminase (SGPT) and serum glutamyl oxalacetate transaminase (SGOT) levels compared to controls. Rats prefed with<jats:italic>E. coli<\/jats:italic>16 (pUC8:16<jats:italic>gfp<\/jats:italic>) demonstrated near to normal levels for SGPT and SGOT, whereas the liver homogenate catalase activity was significantly increased compared to CCl<jats:sub>4<\/jats:sub>treated rats. Thus, pUC8:16<jats:italic>gfp<\/jats:italic>plasmid encoding<jats:italic>vgb<\/jats:italic>improved the growth and GI tract colonization of<jats:italic>E. coli<\/jats:italic>16. In addition, it also enhanced catalase activity in rats harboring<jats:italic>E. coli<\/jats:italic>16 (pUC8:16<jats:italic>gfp<\/jats:italic>), thereby preventing the absorption of CCl<jats:sub>4<\/jats:sub>to GI tract.<\/jats:p>","DOI":"10.1155\/2014\/213574","type":"journal-article","created":{"date-parts":[[2014,6,22]],"date-time":"2014-06-22T10:35:42Z","timestamp":1403433342000},"page":"1-9","source":"Crossref","is-referenced-by-count":7,"title":["Potential Probiotic<i>Escherichia coli<\/i>16 Harboring the<i>Vitreoscilla<\/i>Hemoglobin Gene Improves Gastrointestinal Tract Colonization and Ameliorates Carbon Tetrachloride Induced Hepatotoxicity in Rats"],"prefix":"10.1155","volume":"2014","author":[{"given":"Prasant","family":"Kumar","sequence":"first","affiliation":[{"name":"Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara Gujarat 390002, India"},{"name":"C.G. Bhakta Institute of Biotechnology, Faculty of Applied Science, UKA Tarsadia University, Bardoli, Surat Gujarat 394 350, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ayush V.","family":"Ranawade","sequence":"additional","affiliation":[{"name":"Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara Gujarat 390002, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naresh G.","family":"Kumar","sequence":"additional","affiliation":[{"name":"Molecular Microbial Biochemistry Laboratory, Department of Biochemistry, Faculty of Science, Maharaja Sayajirao University of Baroda, Vadodara Gujarat 390002, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"98","reference":[{"issue":"14","key":"1","doi-asserted-by":"crossref","first-page":"3495","DOI":"10.1128\/JB.180.14.3495-3502.1998","volume":"180","year":"1998","journal-title":"Journal of Bacteriology"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1038\/448542a"},{"issue":"4","key":"3","doi-asserted-by":"crossref","first-page":"1157","DOI":"10.1128\/MMBR.62.4.1157-1170.1998","volume":"62","year":"1998","journal-title":"Microbiology and Molecular Biology Reviews"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1126\/science.1110591"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s11739-010-0451-0"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506655103"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1038\/nature05414"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro2298"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pbio.0050177"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2006.05.005"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2006.02834.x"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-008-1726-5"},{"issue":"2","key":"13","doi-asserted-by":"crossref","first-page":"112","DOI":"10.3844\/ajidsp.2009.112.117","volume":"5","year":"2009","journal-title":"The American Journal of Infectious Diseases"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.8.4586"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1002\/bit.10440"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.00484-07"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2008.09.018"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(03)00083-4"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20091417"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.2478\/s11756-011-0099-x"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-006-0190-x"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbiosc.2010.07.001"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21097"},{"key":"24","year":"2001"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(84)05016-3"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.01.007"},{"issue":"1","key":"27","doi-asserted-by":"crossref","first-page":"265","DOI":"10.1016\/S0021-9258(19)52451-6","volume":"193","year":"1951","journal-title":"The Journal of Biological Chemistry"},{"issue":"2","key":"28","doi-asserted-by":"crossref","first-page":"351","DOI":"10.1016\/0003-2697(79)90738-3","volume":"95","year":"1979","journal-title":"Analytical Biochemistry"},{"issue":"3","key":"29","doi-asserted-by":"crossref","first-page":"288","DOI":"10.1021\/bp00033a008","volume":"11","year":"1995","journal-title":"Biotechnology Progress"},{"issue":"6157","key":"30","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1038\/331633a0","volume":"331","year":"1988","journal-title":"Nature"},{"issue":"14","key":"31","doi-asserted-by":"crossref","first-page":"4149","DOI":"10.1093\/nar\/18.14.4149","volume":"18","year":"1990","journal-title":"Nucleic Acids Research"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(90)90002-T"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0893-926"},{"issue":"2","key":"34","first-page":"71","volume":"4","year":"2008","journal-title":"International Journal of Biological Sciences"},{"issue":"25","key":"35","doi-asserted-by":"crossref","first-page":"12224","DOI":"10.1016\/S0021-9258(18)37743-3","volume":"263","year":"1988","journal-title":"Journal of Biological Chemistry"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00482-06"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47403-0"},{"issue":"4","key":"38","first-page":"208","volume":"2","year":"2006","journal-title":"International Journal of Biological Sciences"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/213574.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/213574.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/213574.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,9]],"date-time":"2022-04-09T02:14:23Z","timestamp":1649470463000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2014\/213574\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":38,"alternative-id":["213574","213574"],"URL":"https:\/\/doi.org\/10.1155\/2014\/213574","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}