{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T22:16:46Z","timestamp":1767651406731,"version":"3.41.2"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,6,16]],"date-time":"2014-06-16T00:00:00Z","timestamp":1402876800000},"content-version":"vor","delay-in-days":166,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30973814","81273894"],"award-info":[{"award-number":["30973814","81273894"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30973814","81273894"],"award-info":[{"award-number":["30973814","81273894"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Evidence-Based Complementary and Alternative Medicine"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:p><jats:italic>Aim<\/jats:italic>. The present study aims to investigate the effects of nourishing \u201cYin\u201d\u2010removing \u201cFire\u201d Chinese herb mixture on the hypothalamic NKB\/NK3R expression in female precocious model rats. <jats:italic>Materials and Methods<\/jats:italic>. Female Sprague\u2010Dawley rats were randomly divided into four groups: normal (N), central precocious puberty (CPP) model (M), CPP fed with Chinese herbal mixture (CHM), and CPP fed with normal saline (MS). Rats on postnatal day 5 were given a single subcutaneous injection of 300\u2009<jats:italic>\u03bc<\/jats:italic>g to establish CPP model rats. Rats of CHM and MS groups were continuously administered with nourishing \u201cYin\u201d\u2010removing \u201cFire\u201d Chinese herb mixture or saline since postnatal day 15. The expressions of hypothalamic NKB\/NK3R were detected by means of real\u2010time PCR, western blot, and immunofluorescence histochemistry. <jats:italic>Results<\/jats:italic>. The day of vaginal opening and establishment of two regular estrous cycles were delayed in the CHM group compared with M and MS groups. The expression of hypothalamic NKB\/NK3R mRNA and protein in the arcuate nucleus (ARC) and medial preoptic (MPO) area were decreased significantly in the CHM group compared with the M and MS groups on the day of onset\u2010puberty. <jats:italic>Conclusions<\/jats:italic>. These results indicate that the NKB\/NK3R signaling pathway might be involved in the effect of herbal mixture treatment on CPP.<\/jats:p>","DOI":"10.1155\/2014\/217424","type":"journal-article","created":{"date-parts":[[2014,6,17]],"date-time":"2014-06-17T01:41:57Z","timestamp":1402969317000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Effect of Nourishing \u201cYin\u201d\u2010Removing \u201cFire\u201d Chinese Herbal Mixture on Hypothalamic NKB\/NK3R Expression in Female Precocious Rats"],"prefix":"10.1155","volume":"2014","author":[{"given":"Shiran","family":"Wang","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liting","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Yu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhanzhuang","family":"Tian","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,6,16]]},"reference":[{"key":"e_1_2_9_1_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2431-8-27"},{"key":"e_1_2_9_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.02.020"},{"key":"e_1_2_9_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/cne.20626"},{"key":"e_1_2_9_4_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2009-2600"},{"key":"e_1_2_9_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainres.2010.08.087"},{"key":"e_1_2_9_6_2","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.11-002S"},{"key":"e_1_2_9_7_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00517.2010"},{"key":"e_1_2_9_8_2","doi-asserted-by":"publisher","DOI":"10.1262\/jrd.2011-038"},{"key":"e_1_2_9_9_2","doi-asserted-by":"publisher","DOI":"10.3389\/fendo.2012.00048"},{"key":"e_1_2_9_10_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0223"},{"key":"e_1_2_9_11_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.5848-09.2010"},{"key":"e_1_2_9_12_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.87.5.2090"},{"key":"e_1_2_9_13_2","first-page":"57","article-title":"Growth hormone and GnRHa combination therapy in the management of precocious puberty","volume":"42","author":"Khadilkar V. 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