{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:11:28Z","timestamp":1788869488627,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, Bras\u00edlia, Brazil","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Foundation of the State of Minas Gerais, Belo Horizonte, Brazil"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>\n                    Infectious epididymitis is considered a major cause of economic losses for the sheep industry worldwide. This study aimed to investigate clinical and pathological changes associated with experimental infections with\n                    <jats:italic>A. seminis<\/jats:italic>\n                    and\n                    <jats:italic>H. somni<\/jats:italic>\n                    in rams. Twenty rams of age 18 to 24 months were infected by intraepididymal inoculation of\n                    <jats:italic>A. seminis<\/jats:italic>\n                    (\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                      <mml:mrow>\n                        <mml:mi>n<\/mml:mi>\n                        <mml:mo>=<\/mml:mo>\n                        <mml:mn>10<\/mml:mn>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    ) and\n                    <jats:italic>H. somni<\/jats:italic>\n                    (\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                      <mml:mrow>\n                        <mml:mi>n<\/mml:mi>\n                        <mml:mo>=<\/mml:mo>\n                        <mml:mn>10<\/mml:mn>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    ). Rams were weekly examined and biological samples were collected during six weeks. All rams inoculated with\n                    <jats:italic>A. seminis<\/jats:italic>\n                    and 80% inoculated with\n                    <jats:italic>H. somni<\/jats:italic>\n                    became infected. The recovery of bacteria was possible in semen and urine samples and tissues in both experimental groups. Clinically, there were a decrease in testicular consistency and an increase in measures of the left epididymis tails in both experimental groups. The main gross changes were observed in the reproductive tract. Microscopically, the main lesions were inflammatory changes in the genitourinary tract and testicular degeneration.\n                    <jats:italic>A. seminis<\/jats:italic>\n                    and\n                    <jats:italic>H. somni<\/jats:italic>\n                    were able to colonize several organs of the genitourinary tract in rams, being indistinguishable by clinical exam, necropsy or histopathology. For differential diagnosis, it is important to use diagnostic techniques for direct confirmation of the etiologic agent.\n                  <\/jats:p>","DOI":"10.1155\/2014\/241452","type":"journal-article","created":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T16:10:48Z","timestamp":1390839048000},"page":"1-10","source":"Crossref","is-referenced-by-count":19,"title":["Clinical and Pathological Changes in Rams Experimentally Infected with\n                    <i>Actinobacillus seminis<\/i>\n                    and\n                    <i>Histophilus somni<\/i>"],"prefix":"10.1155","volume":"2014","author":[{"given":"Val\u00e9ria S.","family":"Moustacas","sequence":"first","affiliation":[{"name":"Departamento de Cl\u00ednica e Cirurgia Veterin\u00e1rias, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teane M. A.","family":"Silva","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednica e Cirurgia Veterin\u00e1rias, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luciana F.","family":"Costa","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Geral, Instituto de Ci\u00eancias Biol\u00f3gicas da Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos, 6627, 31270-901 Belo Horizonte, MG, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cust\u00f3dio A.","family":"Carvalho J\u00fanior","sequence":"additional","affiliation":[{"name":"Departamento de Cl\u00ednica e Cirurgia Veterin\u00e1rias, Escola de Veterin\u00e1ria, Universidade Federal de Minas Gerais, Avenida Ant\u00f4nio Carlos, 6627, 31270-901 Belo Horizonte, MG, 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