{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,5]],"date-time":"2026-07-05T00:20:37Z","timestamp":1783210837461,"version":"3.54.6"},"reference-count":51,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Long-Term Research Grant Scheme","award":["203\/PSK\/6722001"],"award-info":[{"award-number":["203\/PSK\/6722001"]}]},{"name":"Long-Term Research Grant Scheme","award":["PICSA12-173"],"award-info":[{"award-number":["PICSA12-173"]}]},{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["203\/PSK\/6722001"],"award-info":[{"award-number":["203\/PSK\/6722001"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003093","name":"Ministry of Higher Education, Malaysia","doi-asserted-by":"publisher","award":["PICSA12-173"],"award-info":[{"award-number":["PICSA12-173"]}],"id":[{"id":"10.13039\/501100003093","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Science and Technology, Cuba","award":["203\/PSK\/6722001"],"award-info":[{"award-number":["203\/PSK\/6722001"]}]},{"name":"Ministry of Science and Technology, Cuba","award":["PICSA12-173"],"award-info":[{"award-number":["PICSA12-173"]}]},{"name":"Institute of Science and Technology of Mexico City","award":["203\/PSK\/6722001"],"award-info":[{"award-number":["203\/PSK\/6722001"]}]},{"name":"Institute of Science and Technology of Mexico City","award":["PICSA12-173"],"award-info":[{"award-number":["PICSA12-173"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>A more effective vaccine against tuberculosis (TB) is urgently needed. Based on its high genetic homology with<jats:italic>Mycobacterium tuberculosis<\/jats:italic>(Mtb), the nonpathogenic mycobacteria,<jats:italic>Mycobacterium smegmatis<\/jats:italic>(Ms), could be an attractive source of potential antigens to be included in such a vaccine. We evaluated the capability of lipid-based preparations obtained from Ms to provide a protective response in Balb\/c mice after challenge with Mtb H37Rv strain. The intratracheal model of progressive pulmonary TB was used to assess the level of protection in terms of bacterial load as well as the pathological changes in the lungs of immunized Balb\/c mice following challenge with Mtb. Mice immunized with the lipid-based preparation from Ms either adjuvanted with Alum (LMs-AL) or nonadjuvanted (LMs) showed significant reductions in bacterial load (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.01<\/mml:mn><\/mml:math>) compared to the negative control group (animals immunized with phosphate buffered saline (PBS)). Both lipid formulations showed the same level of protection as Bacille Calmette and Guerin (BCG). Regarding the pathologic changes in the lungs, mice immunized with both lipid formulations showed less pneumonic area when compared with the PBS group (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn fontstyle=\"italic\">0.01<\/mml:mn><\/mml:math>) and showed similar results compared with the BCG group. These findings suggest the potential of LMs as a promising vaccine candidate against TB.<\/jats:p>","DOI":"10.1155\/2014\/273129","type":"journal-article","created":{"date-parts":[[2014,12,7]],"date-time":"2014-12-07T16:01:25Z","timestamp":1417968085000},"page":"1-6","source":"Crossref","is-referenced-by-count":7,"title":["Protective Effect of a Lipid-Based Preparation from<i>Mycobacterium smegmatis<\/i>in a Murine Model of Progressive Pulmonary Tuberculosis"],"prefix":"10.1155","volume":"2014","author":[{"given":"Maria de los Angeles","family":"Garc\u00eda","sequence":"first","affiliation":[{"name":"Instituto Finlay, 11600 Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0679-7973","authenticated-orcid":true,"given":"Reinier","family":"Borrero","sequence":"additional","affiliation":[{"name":"Instituto Finlay, 11600 Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria E.","family":"Lanio","sequence":"additional","affiliation":[{"name":"Center for Protein Studies, Faculty of Biology, Havana University, 10400 Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanely","family":"Tirado","sequence":"additional","affiliation":[{"name":"Instituto Finlay, 11600 Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadine","family":"Alvarez","sequence":"additional","affiliation":[{"name":"Instituto Finlay, 11600 Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alina","family":"Puig","sequence":"additional","affiliation":[{"name":"Instituto Finlay, 11600 Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alicia","family":"Aguilar","sequence":"additional","affiliation":[{"name":"Instituto Finlay, 11600 Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liem","family":"Canet","sequence":"additional","affiliation":[{"name":"Center for Protein Studies, Faculty of Biology, Havana University, 10400 Havana, Cuba"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dulce","family":"Mata Espinoza","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, 14000 M\u00e9xico, DF, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jorge","family":"Barrios Pay\u00e1n","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, 14000 M\u00e9xico, DF, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mar\u00eda Elena","family":"Sarmiento","sequence":"additional","affiliation":[{"name":"Instituto Finlay, 11600 Havana, Cuba"},{"name":"School of Health Sciences, Universiti Sains Malaysia, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rogelio","family":"Hern\u00e1ndez-Pando","sequence":"additional","affiliation":[{"name":"Instituto Nacional de Ciencias M\u00e9dicas y Nutrici\u00f3n Salvador Zubir\u00e1n, 14000 M\u00e9xico, DF, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohd-Nor","family":"Norazmi","sequence":"additional","affiliation":[{"name":"School of Health Sciences, Universiti Sains Malaysia, Malaysia"},{"name":"Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Armando","family":"Acosta","sequence":"additional","affiliation":[{"name":"Instituto Finlay, 11600 Havana, Cuba"},{"name":"Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1200894"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(14)70033-5"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.3114"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/917860"},{"key":"5","first-page":"123","volume-title":"Lipid adjuvants and antigens embedded in the mycobacterial cell envelope","year":"2014"},{"key":"6","first-page":"949","volume-title":"New TB vaccines. What is the pipeline?","year":"2014"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxg091"},{"issue":"3","key":"8","first-page":"160","volume":"30","year":"1993","journal-title":"Indian Journal of Biochemistry and Biophysics"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-695X.1994.tb00433.x"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-14-S1-S11"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-14-S1-S13"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.06.077"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-14-S1-S7"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/0041-3879(70)90073-5"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1139\/o59-099"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1184-979"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2012.11.042"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1086\/426453"},{"key":"19","year":"1984"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4614-6111-1_11"},{"key":"21","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1201667"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1172\/JCI46216"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900858"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0804044"},{"key":"25","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2012.01166.x"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0403787101"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2006.01.006"},{"key":"28","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2009.02.003"},{"key":"29","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2008.10.007"},{"issue":"2","key":"30","first-page":"66","volume":"17","year":"2010","journal-title":"Malaysian Journal of Medical Sciences"},{"key":"31","doi-asserted-by":"publisher","DOI":"10.1016\/j.provac.2010.07.011"},{"key":"32","volume-title":"Towards a new challenge in TB control: development of antibody-based protection","year":"2012"},{"key":"33","volume":"14","year":"2013","journal-title":"BMC immunology"},{"key":"34","volume-title":"The role of antibodies in the defense against tuberculosis","year":"2013"},{"key":"35","first-page":"239","volume-title":"The role of antibodies against TB","year":"2014"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.9.6101-6109.2005"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2011.01.110"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.08.029"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1080\/08820130701690782"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003116"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1080\/08982100902726820"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.1021\/mp100208f"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2011.05.019"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1002\/jps.22427"},{"key":"45","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamem.2005.10.011"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0005771"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0804091"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejpb.2010.10.001"},{"key":"49","first-page":"985","volume-title":"Mucosal immunity and vaccination with special emphasis on respiratory tract","year":"2014"},{"key":"50","first-page":"1027","volume-title":"Inhalable vaccines for TB","year":"2014"},{"key":"51","first-page":"999","volume-title":"Respiratory mucosal immunization against pulmonary TB","year":"2014"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/273129.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/273129.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/273129.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2016,8,18]],"date-time":"2016-08-18T11:18:11Z","timestamp":1471519091000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2014\/273129\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":51,"alternative-id":["273129","273129"],"URL":"https:\/\/doi.org\/10.1155\/2014\/273129","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}