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During progression of gastric cancer (GC), degradation of the extracellular matrix is mediated by the matrix metalloproteases (MMPs) and their tissue inhibitors (TIMPs): changes in the expression of these have been related to unfavorable prognosis in GC.<jats:italic>Objective<\/jats:italic>. To analyze the expression of certain MMPs and TIMPs in chronic superficial gastritis (SG) and GC.<jats:italic>Methods<\/jats:italic>. The expression of MMPs and TIMPs was determined using qRT-PCR; the expression was classified, using threshold cycle (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:msub><mml:mrow><mml:mi>C<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>) values, as very high (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:msub><mml:mrow><mml:mi>C<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><\/mml:msub><mml:mo>\u2264<\/mml:mo><mml:mn>25<\/mml:mn><\/mml:math>), high (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:msub><mml:mrow><mml:mi>C<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><\/mml:msub><mml:mo>=<\/mml:mo><mml:mn>26<\/mml:mn><mml:mtext>\u2013<\/mml:mtext><mml:mn>30<\/mml:mn><\/mml:math>), moderate (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:msub><mml:mrow><mml:mi>C<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><\/mml:msub><mml:mo>=<\/mml:mo><mml:mn>31<\/mml:mn><mml:mtext>\u2013<\/mml:mtext><mml:mn>35<\/mml:mn><\/mml:math>), low (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:msub><mml:mrow><mml:mi>C<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><\/mml:msub><mml:mo>=<\/mml:mo><mml:mn>36<\/mml:mn><mml:mtext>\u2013<\/mml:mtext><mml:mn>39<\/mml:mn><\/mml:math>), or not detected (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:msub><mml:mrow><mml:mi>C<\/mml:mi><\/mml:mrow><mml:mrow><mml:mi>T<\/mml:mi><\/mml:mrow><\/mml:msub><mml:mo>=<\/mml:mo><mml:mn>40<\/mml:mn><\/mml:math>). Strength of association was estimated between the proteins, which were detected by Western blot, and the risk of developing GC.<jats:italic>Results<\/jats:italic>. We found a high expression of<jats:italic>MMP1<\/jats:italic>,<jats:italic>MMP2<\/jats:italic>,<jats:italic>MMP14<\/jats:italic>,<jats:italic>TIMP1,<\/jats:italic>and<jats:italic>TIMP3<\/jats:italic>; moderate one of<jats:italic>MMP9<\/jats:italic>and<jats:italic>MMP25,<\/jats:italic>and low one of<jats:italic>MMP13<\/jats:italic>and<jats:italic>MMP24<\/jats:italic>in both tissues. In absolute mRNA levels, significant differences were found in expression of<jats:italic>MMP2<\/jats:italic>,<jats:italic>MMP24,<\/jats:italic>and<jats:italic>MMP25<\/jats:italic>, which are overexpressed in GC compared with SG. The presence of the proteins MMP-14 and TIMP-3 was associated with the risk of developing GC.<jats:italic>Conclusions<\/jats:italic>. We consider that<jats:italic>MMP2<\/jats:italic>,<jats:italic>MMP24,<\/jats:italic>and<jats:italic>MMP25<\/jats:italic>and the proteins MMP-14 and TIMP-3 could be candidates for prognostic molecular markers in GC.<\/jats:p>","DOI":"10.1155\/2014\/285906","type":"journal-article","created":{"date-parts":[[2014,2,11]],"date-time":"2014-02-11T16:01:34Z","timestamp":1392134494000},"page":"1-9","source":"Crossref","is-referenced-by-count":17,"title":["Expression of the Matrix Metalloproteases 2, 14, 24, and 25 and Tissue Inhibitor 3 as Potential Molecular Markers in Advanced Human Gastric Cancer"],"prefix":"10.1155","volume":"2014","author":[{"given":"Sol","family":"de la Pe\u00f1a","sequence":"first","affiliation":[{"name":"Biomedical Sciences Doctoral Program, Biomedical Research Center, University of Veracruz, 91190 Xalapa, Veracruz, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clara 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