{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T23:37:32Z","timestamp":1788910652507,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001332","name":"Council for Scientific and Industrial Research","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001332","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>The study investigated the growth-inhibiting and apoptosis mediating effects of<jats:italic>B. serrata<\/jats:italic>extract as monotherapy and combination therapy with DOX against hepatocellular carcinoma cell lines. Boswellic acid rich fraction of<jats:italic>B. serrata<\/jats:italic>extract was prepared. MTT assay on HepG2 and Hep3B cells was carried out using<jats:italic>B. serrata<\/jats:italic>alone and in combination with DOX. Further, caspase-3 activity, TNF-<jats:italic>\u03b1<\/jats:italic>level, and IL-6 level were estimated. Isobolographic analysis was carried out to evaluate the effect of combination therapy. Additionally, protective effect of<jats:italic>B. serrata<\/jats:italic>extract on DOX induced hepatic toxicity was also evaluated in Wistar rats.<jats:italic>B. serrata<\/jats:italic>extract inhibited growth of HepG2 (IC50 value of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mn>21.21<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.92<\/mml:mn><\/mml:math>\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) as well as HepG2 (IC50 value of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mn>18.65<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.71<\/mml:mn><\/mml:math>\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL). DOX inhibited growth in HepG2 and Hep3B cells with an IC50 of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mn>1.06<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.04<\/mml:mn><\/mml:math>\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mn>1.92<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.09<\/mml:mn><\/mml:math>\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL. Isobolographic analysis showed combination index (CI) of DOX and<jats:italic>B. serrata<\/jats:italic>extract of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mn>0.53<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.03<\/mml:mn><\/mml:math>to<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M6\"><mml:mn>0.79<\/mml:mn><mml:mo>\u00b1<\/mml:mo><mml:mn>0.02<\/mml:mn><\/mml:math>suggesting synergistic behavior against the two cell lines.<jats:italic>B. serrata<\/jats:italic>extract also caused dose dependent increase in caspase-3 activity, TNF-<jats:italic>\u03b1<\/jats:italic>level, and IL-6 level which was higher (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M7\"><mml:mi>P<\/mml:mi><mml:mo>&lt;<\/mml:mo><mml:mn>0.001<\/mml:mn><\/mml:math>) with DOX (1\u2009<jats:italic>\u03bc<\/jats:italic>M) and<jats:italic>B. serrata<\/jats:italic>extract (20\u2009<jats:italic>\u03bc<\/jats:italic>g\/mL) combination.<jats:italic>B. serrata<\/jats:italic>extract also protected Wistar rats against DOX induced hepatic toxicity.<\/jats:p>","DOI":"10.1155\/2014\/294143","type":"journal-article","created":{"date-parts":[[2014,8,9]],"date-time":"2014-08-09T21:02:38Z","timestamp":1407618158000},"page":"1-11","source":"Crossref","is-referenced-by-count":25,"title":["Caspase Mediated Synergistic Effect of<i>Boswellia serrata<\/i>Extract in Combination with Doxorubicin against Human Hepatocellular Carcinoma"],"prefix":"10.1155","volume":"2014","author":[{"given":"Mohammad Ahmed","family":"Khan","sequence":"first","affiliation":[{"name":"Department of Pharmacology, Faculty of Pharmacy, Hamdard University, Hamdard Nagar, New Delhi 110062, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mhaveer","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy & Phytochemistry, Faculty of Pharmacy, Hamdard University (Jamia Hamdard), Hamdard Nagar, New Delhi 110062, 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