{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T21:21:06Z","timestamp":1740172866766,"version":"3.37.3"},"reference-count":16,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,9]],"date-time":"2014-01-09T00:00:00Z","timestamp":1389225600000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pathology Research International"],"published-print":{"date-parts":[[2014,1,9]]},"abstract":"Objective<\/jats:italic>. To evaluate trophoblastic cell proliferation and angiogenesis in tubal pregnancy assessed by immunohistochemical study and their correlation with an average variation of \u03b2<\/jats:italic>-hCG in an interval of 48 hours before surgery. Methods<\/jats:italic>. A prospective study was conducted on 18 patients with a diagnosis of tubal pregnancy. The patients were divided into two groups of ectopic pregnancy of which 11 showed rise of \u03b2<\/jats:italic>-hCG levels and 7 patients showed declining \u03b2<\/jats:italic>-hCG levels in an interval of 48 hours prior to surgery. Trophoblastic cell proliferation and angiogenesis were assessed by Ki-67 and VEGF, respectively. Trophoblastic cell proliferation was assessed by Ki-67 and was classified into three groups (grade I: less than 1\/3 of stained nuclei, grade II: 1\/3 to 2\/3 of the stained nuclei, and grade III: more than 2\/3 of the nuclei stained). The cases analyzed for VEGF were divided into three groups (grade I: less than 1\/3 of the stained cytoplasm; grade II: 1\/3 to 2\/3 of the stained cytoplasm; grade III: more than 2\/3 of the stained cytoplasm). Statistical analysis was performed using the chi-square, ANOVA, and Kruskal-Wallis tests. Results<\/jats:italic>. The mean variation in the serum \u03b2<\/jats:italic>-hCG levels in 48 hours in tubal pregnancy patients correlated with trophoblastic cell proliferation assessed by Ki-67 and showed a decline of 13.46% in grade I, a rise of 45.99% in grade II, and ascension of 36.68% in grade III (P<\/mml:mi>=<\/mml:mo>0.030<\/mml:mn><\/mml:math>). The average variation in the serum \u03b2<\/jats:italic>-hCG in 48 hours, where angiogenesis was evaluated by VEGF, showed a decline of 18.35% in grade I, a rise of 32.95% in grade II, and ascension of 37.55% in grade III (P<\/mml:mi>=<\/mml:mo>0.047<\/mml:mn><\/mml:math>). Conclusions<\/jats:italic>. Our observations showed a direct correlation of increased levels of serum \u03b2<\/jats:italic>-hCG in 48h period prior to surgery with higher trophoblastic cell proliferation assessed by Ki-67 and angiogenesis assessed by VEGF in tubal pregnancy.<\/jats:p>","DOI":"10.1155\/2014\/302634","type":"journal-article","created":{"date-parts":[[2014,1,9]],"date-time":"2014-01-09T21:03:43Z","timestamp":1389301423000},"page":"1-5","source":"Crossref","is-referenced-by-count":0,"title":["Trophoblastic Infiltration in Tubal Pregnancy Evaluated by Immunohistochemistry and Correlation with Variation of Beta-Human Chorionic Gonadotropin"],"prefix":"10.1155","volume":"2014","author":[{"given":"Danyelle Farias","family":"Ferreira","sequence":"first","affiliation":[{"name":"Department of Obstetrics, S\u00e3o Paulo Federal University (UNIFESP), Rua Carlos Weber 956, Apartamento 113 Visage, Alto da Lapa, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Julio","family":"Elito J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, S\u00e3o Paulo Federal University (UNIFESP), Rua Carlos Weber 956, Apartamento 113 Visage, Alto da Lapa, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6145-2532","authenticated-orcid":true,"given":"Edward","family":"Araujo J\u00fanior","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, S\u00e3o Paulo Federal University (UNIFESP), Rua Carlos Weber 956, Apartamento 113 Visage, Alto da Lapa, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jo\u00e3o Norberto","family":"Stavale","sequence":"additional","affiliation":[{"name":"Department of Pathology, S\u00e3o Paulo Federal University (UNIFESP), S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Luiz","family":"Camano","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, S\u00e3o Paulo Federal University (UNIFESP), Rua Carlos Weber 956, Apartamento 113 Visage, Alto da Lapa, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio Fernandes","family":"Moron","sequence":"additional","affiliation":[{"name":"Department of Obstetrics, S\u00e3o Paulo Federal University (UNIFESP), Rua Carlos Weber 956, Apartamento 113 Visage, Alto da Lapa, S\u00e3o Paulo, SP, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"crossref","first-page":"379","DOI":"10.1056\/NEJMcp0810384","volume":"361","year":"2009","journal-title":"The New England Journal of 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