{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,21]],"date-time":"2026-02-21T02:47:36Z","timestamp":1771642056830,"version":"3.50.1"},"reference-count":48,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"CAPES","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100004911","name":"FAPEPI","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004911","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100005279","name":"Universidade Federal do Piau\u00ed","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005279","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>The<jats:italic>Lippia origanoides<\/jats:italic>H.B.K. ethanol extract (LOEE) and hexane (LOHEX), dichloromethane (LODCM), and ethyl acetate (LOEA) fractions were tested for their antimicrobial activity alone or in combination with antibiotics against a methicillin resistant<jats:italic>Staphylococcus aureus<\/jats:italic>(MRSA) strain. The natural products did not show antimicrobial activity against multidrug resistant strain at the clinically significant concentrations tested. However, a modulatory effect in the antibacterial activity of the neomycin and amikacin was verified when LOEE, LOHEX and LODCM were added to the growth medium at subinhibitory concentrations. A similar modulation was found when the natural products were changed for chlorpromazine, an inhibitor of bacterial efflux pumps, suggesting the involvement of resistance mediated by efflux system in the MRSA tested. The fractions LOHEX and LODCM showed a modulatory activity bigger than their majority compounds (carvacrol, thymol, and naringenin), indicating that this activity is not due to their majority compounds only, but it is probably due to a synergism between their chemical components. These results indicate that<jats:italic>L. origanoides<\/jats:italic>H.B.K. can be a source of phytochemicals able to modify the phenotype of resistance to aminoglycosides in MRSA.<\/jats:p>","DOI":"10.1155\/2014\/305610","type":"journal-article","created":{"date-parts":[[2014,2,10]],"date-time":"2014-02-10T16:01:41Z","timestamp":1392048101000},"page":"1-7","source":"Crossref","is-referenced-by-count":30,"title":["Phytochemical Prospection and Modulation of Antibiotic Activity In Vitro by<i>Lippia origanoides<\/i>H.B.K. in Methicillin Resistant<i>Staphylococcus aureus<\/i>"],"prefix":"10.1155","volume":"2014","author":[{"given":"Humberto","family":"Medeiros Barreto","sequence":"first","affiliation":[{"name":"Laboratory of Microbiology and Immunology, Campus Am\u00edlcar Ferreira Sobral, Universidade Federal do Piau\u00ed, BR 343, Km 3,5 Bairro Melad\u00e3o, 64800-000 Floriano, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filipe","family":"Cerqueira Fontinele","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Immunology, Campus Am\u00edlcar Ferreira Sobral, Universidade Federal do Piau\u00ed, BR 343, Km 3,5 Bairro Melad\u00e3o, 64800-000 Floriano, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alde\u00eddia","family":"Pereira de Oliveira","sequence":"additional","affiliation":[{"name":"Center for Research on Medicinal Plants, Universidade Federal do Piau\u00ed, Bairro Ininga, 64049-550 Teresina, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel Dias Rufino","family":"Arcanjo","sequence":"additional","affiliation":[{"name":"Center for Research on Medicinal Plants, Universidade Federal do Piau\u00ed, Bairro Ininga, 64049-550 Teresina, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernadete Helena","family":"Cavalcanti dos Santos","sequence":"additional","affiliation":[{"name":"Laboratory of Clinical Microbiology, Universidade Federal da Paraiba, Cidade Universit\u00e1ria, 58051-900 Jo\u00e3o Pessoa, PB, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aislan Pereira Lira","family":"de Abreu","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculdade de Ensino Superior de Floriano, Bairro Manguinha, 64800-000 Floriano, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique","family":"Douglas Melo Coutinho","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology and Molecular Biology, Regional University of Cariri, Bairro Pimenta, 63105-100 Crato, CE, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Romezio","family":"Alves Carvalho da Silva","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products, Universidade Federal do Piau\u00ed, Bairro Ininga, 64049-550 Teresina, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Taciana","family":"Oliveira de Sousa","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products, Universidade Federal do Piau\u00ed, Bairro Ininga, 64049-550 Teresina, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria das Gra\u00e7as","family":"Freire de Medeiros","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products, Universidade Federal do Piau\u00ed, Bairro Ininga, 64049-550 Teresina, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonia Maria das Gra\u00e7as","family":"Lopes Cit\u00f3","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products, Universidade Federal do Piau\u00ed, Bairro Ininga, 64049-550 Teresina, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos\u00e9 Arimateia","family":"Dantas Lopes","sequence":"additional","affiliation":[{"name":"Laboratory of Natural Products, Universidade Federal do Piau\u00ed, Bairro Ininga, 64049-550 Teresina, PI, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"1","key":"1","doi-asserted-by":"crossref","first-page":"32","DOI":"10.17305\/bjbms.2010.2733","volume":"10","year":"2010","journal-title":"Bosnian Journal of Basic Medical Sciences"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1590\/S0365-05962009000500011"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.011452-0"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.micro.112408.134309"},{"issue":"10","key":"5","first-page":"36","volume":"41","year":"2009","journal-title":"Emergency Medicine"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.11.156"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13044484"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkt045"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2012.05.112"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1159\/000328975"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2011.04.054"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1055\/s-2008-1074518"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1590\/S0102-695X2011005000013"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1002\/jssc.200900452"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-8741(01)00234-3"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.jspr.2012.05.002"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2012.06.003"},{"key":"18","first-page":"306","volume":"60","year":"2011","journal-title":"Acta Agronomica"},{"key":"19","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1016\/S0123-9392(11)70080-7","volume":"15","year":"2011","journal-title":"Infection"},{"key":"20","doi-asserted-by":"publisher","DOI":"10.1016\/j.fitote.2009.10.008"},{"issue":"5","key":"21","doi-asserted-by":"crossref","first-page":"504","DOI":"10.1080\/10412905.2004.9698782","volume":"16","year":"2004","journal-title":"Journal of Essential Oil Research"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2008.12.025"},{"key":"23","doi-asserted-by":"publisher","DOI":"10.1021\/jm9509410"},{"key":"24","doi-asserted-by":"publisher","DOI":"10.1089\/jmf.2009.0158"},{"issue":"5","key":"25","doi-asserted-by":"crossref","first-page":"834","DOI":"10.1590\/S0100-40422005000500020","volume":"28","year":"2005","journal-title":"Quimica Nova"},{"key":"26","doi-asserted-by":"publisher","DOI":"10.1078\/094471103322331476"},{"key":"27","doi-asserted-by":"publisher","DOI":"10.3109\/13880200903382686"},{"issue":"6","key":"28","first-page":"1049","volume":"29","year":"2010","journal-title":"Latin American Journal of Pharmacy"},{"issue":"2","key":"29","first-page":"287","volume":"23","year":"2009","journal-title":"In Vivo"},{"issue":"4","key":"30","doi-asserted-by":"crossref","first-page":"727","DOI":"10.1128\/AAC.43.4.727","volume":"43","year":"1999","journal-title":"Antimicrobial Agents and Chemotherapy"},{"issue":"3","key":"31","first-page":"389","volume":"135","year":"2012","journal-title":"Indian Journal of Medical Research"},{"key":"32","doi-asserted-by":"publisher","DOI":"10.2174\/1874285801307010059"},{"key":"33","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00580-10"},{"key":"34","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.13.16"},{"key":"35","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.2.719-726.2003"},{"key":"36","doi-asserted-by":"publisher","DOI":"10.1016\/j.foodchem.2008.05.060"},{"key":"37","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(03)00890-5"},{"key":"38","doi-asserted-by":"publisher","DOI":"10.1590\/S1517-83822013005000001"},{"key":"39","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2001.01428.x"},{"key":"40","doi-asserted-by":"publisher","DOI":"10.1016\/0378-8741(96)85514-0"},{"key":"41","doi-asserted-by":"publisher","DOI":"10.1021\/jf901456u"},{"key":"42","doi-asserted-by":"publisher","DOI":"10.3390\/molecules16064828"},{"key":"43","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2695"},{"key":"44","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.6.1968-1973.2004"},{"key":"45","first-page":"497","volume":"63","year":"2012","journal-title":"Journal of Physiology and Pharmacology"},{"key":"46","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2007.03456.x"},{"key":"47","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2011.05070.x"},{"key":"48","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2008.09.001"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/305610.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/305610.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/305610.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,7]],"date-time":"2019-08-07T10:05:19Z","timestamp":1565172319000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2014\/305610\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":48,"alternative-id":["305610","305610"],"URL":"https:\/\/doi.org\/10.1155\/2014\/305610","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}