{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:24:18Z","timestamp":1791264258193,"version":"4.1.0"},"reference-count":38,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001677","name":"Institut national de la sant\u00e9 et de la recherche m\u00e9dicale","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001677","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>\n                    Angiogenesis is a key process for proper placental development and for the success of pregnancy. Although numerous\n                    <jats:italic>in vitro<\/jats:italic>\n                    methods have been developed for the assessment of this process, relatively few reliable\n                    <jats:italic>in vivo<\/jats:italic>\n                    methods are available to evaluate this activity throughout gestation. Here we report an\n                    <jats:italic>in vivo<\/jats:italic>\n                    technique that specifically measures placental neovascularization. The technique is based on the measurement of a fluorescent alpha\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                      <mml:mrow>\n                        <mml:mi>v<\/mml:mi>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    beta 3 (\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\">\n                      <mml:msub>\n                        <mml:mrow>\n                          <mml:mi>\u03b1<\/mml:mi>\n                        <\/mml:mrow>\n                        <mml:mrow>\n                          <mml:mi>v<\/mml:mi>\n                        <\/mml:mrow>\n                      <\/mml:msub>\n                      <mml:msub>\n                        <mml:mrow>\n                          <mml:mi>\u03b2<\/mml:mi>\n                        <\/mml:mrow>\n                        <mml:mrow>\n                          <mml:mn>3<\/mml:mn>\n                        <\/mml:mrow>\n                      <\/mml:msub>\n                    <\/mml:math>\n                    ) integrin-targeting molecule called Angiolone-Alexa-Fluor 700. The\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\">\n                      <mml:msub>\n                        <mml:mrow>\n                          <mml:mi>\u03b1<\/mml:mi>\n                        <\/mml:mrow>\n                        <mml:mrow>\n                          <mml:mi>v<\/mml:mi>\n                        <\/mml:mrow>\n                      <\/mml:msub>\n                      <mml:msub>\n                        <mml:mrow>\n                          <mml:mi>\u03b2<\/mml:mi>\n                        <\/mml:mrow>\n                        <mml:mrow>\n                          <mml:mn>3<\/mml:mn>\n                        <\/mml:mrow>\n                      <\/mml:msub>\n                    <\/mml:math>\n                    integrin is highly expressed by endothelial cells during the neovascularization and by trophoblast cells during their invasion of the maternal decidua. Angiolone was injected to gravid mice at 6.5 and 11.5 days post coitus (dpc). The fluorescence was analyzed one day later at 7.5 and 12.5\u2009dpc, respectively. We demonstrated that (i) Angiolone targets\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\">\n                      <mml:msub>\n                        <mml:mrow>\n                          <mml:mi>\u03b1<\/mml:mi>\n                        <\/mml:mrow>\n                        <mml:mrow>\n                          <mml:mi>v<\/mml:mi>\n                        <\/mml:mrow>\n                      <\/mml:msub>\n                      <mml:msub>\n                        <mml:mrow>\n                          <mml:mi>\u03b2<\/mml:mi>\n                        <\/mml:mrow>\n                        <mml:mrow>\n                          <mml:mn>3<\/mml:mn>\n                        <\/mml:mrow>\n                      <\/mml:msub>\n                    <\/mml:math>\n                    protein in the placenta with a strong specificity, (ii) this technique is quantitative as the measurement was correlated to the increase of the placental size observed with increasing gestational age, and (iii) information on the outcome is possible, as abnormal placentation could be detected early on during gestation. In conclusion, we report the validation of a new noninvasive and quantitative method to assess the placental angiogenic activity,\n                    <jats:italic>in vivo<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1155\/2014\/309082","type":"journal-article","created":{"date-parts":[[2014,7,10]],"date-time":"2014-07-10T17:10:23Z","timestamp":1405012223000},"page":"1-8","source":"Crossref","is-referenced-by-count":4,"title":["Noninvasive and Quantitative Assessment of\n                    <i>In Vivo<\/i>\n                    Fetomaternal Interface Angiogenesis Using RGD-Based Fluorescence"],"prefix":"10.1155","volume":"2014","author":[{"given":"M.","family":"Keramidas","sequence":"first","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Unit\u00e9 823, Institut Albert Bonniot, Rond-point de la Chantourne, 38700 La Tronche, France"},{"name":"Universit\u00e9 Grenoble Alpes, 38041 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Lavaud","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Unit\u00e9 823, Institut Albert Bonniot, Rond-point de la Chantourne, 38700 La Tronche, France"},{"name":"Universit\u00e9 Grenoble Alpes, 38041 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F.","family":"Sergent","sequence":"additional","affiliation":[{"name":"Commissariat \u00e0 l\u2019\u00c9nergie Atomique et aux \u00c9nergies Alternatives, Institut de Recherches en Sciences et Technologies pour le Vivant (iRTSV), Biologie du Cancer et de l\u2019Infection, 17 rue des Martyrs, 38054 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Hoffmann","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, 38041 Grenoble, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Unit\u00e9 1036, Biologie du Cancer et de l\u2019Infection, 17 rue des Martyrs, 38054 Grenoble, France"},{"name":"CHU de Grenoble, H\u00f4pital Couple Enfant, D\u00e9partement de G\u00e9n\u00e9tique et Procr\u00e9ation, Centre d\u2019Aide M\u00e9dicale \u00e0 la Procr\u00e9ation, BP 217, 38043 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Brouillet","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, 38041 Grenoble, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Unit\u00e9 1036, Biologie du Cancer et de l\u2019Infection, 17 rue des Martyrs, 38054 Grenoble, France"},{"name":"CHU de Grenoble, H\u00f4pital Couple Enfant, D\u00e9partement de G\u00e9n\u00e9tique et Procr\u00e9ation, Centre d\u2019Aide M\u00e9dicale \u00e0 la Procr\u00e9ation, BP 217, 38043 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-J.","family":"Feige","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Grenoble Alpes, 38041 Grenoble, France"},{"name":"Commissariat \u00e0 l\u2019\u00c9nergie Atomique et aux \u00c9nergies Alternatives, Institut de Recherches en Sciences et Technologies pour le Vivant (iRTSV), Biologie du Cancer et de l\u2019Infection, 17 rue des Martyrs, 38054 Grenoble, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Unit\u00e9 1036, Biologie du Cancer et de l\u2019Infection, 17 rue des Martyrs, 38054 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.-L.","family":"Coll","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Unit\u00e9 823, Institut Albert Bonniot, Rond-point de la Chantourne, 38700 La Tronche, France"},{"name":"Universit\u00e9 Grenoble Alpes, 38041 Grenoble, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"N.","family":"Alfaidy","sequence":"additional","affiliation":[{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Unit\u00e9 823, Institut Albert Bonniot, Rond-point de la Chantourne, 38700 La Tronche, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale (INSERM), Unit\u00e9 1036, Biologie du Cancer et de l\u2019Infection, 17 rue des Martyrs, 38054 Grenoble, France"},{"name":"CHU de Grenoble, H\u00f4pital Couple Enfant, D\u00e9partement de G\u00e9n\u00e9tique et Procr\u00e9ation, Centre d\u2019Aide M\u00e9dicale \u00e0 la Procr\u00e9ation, BP 217, 38043 Grenoble, 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