{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T16:15:47Z","timestamp":1790525747414,"version":"4.1.0"},"reference-count":22,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Throat swabs from children with suspected<jats:italic>Mycoplasma pneumoniae<\/jats:italic>(<jats:italic>M. pneumoniae<\/jats:italic>) infection were cultured for the presence of<jats:italic>M. pneumoniae<\/jats:italic>and its species specificity using the 16S rRNA gene. Seventy-six<jats:italic>M. pneumoniae<\/jats:italic>strains isolated from 580 swabs showed that 70 were erythromycin resistant with minimum inhibitory concentrations (MIC) around 32\u2013512\u2009mg\/L. Fifty<jats:italic>M. pneumoniae<\/jats:italic>strains (46 resistant, 4 sensitive) were tested for sensitivity to tetracycline, ciprofloxacin, and gentamicin. Tetracycline and ciprofloxacin had some effect, and gentamicin had an effect on the majority of<jats:italic>M. pneumoniae<\/jats:italic>strains. Domains II and V of the 23S rRNA gene and the ribosomal protein L4 and L22 genes, both of which are considered to be associated with macrolide resistance, were sequenced and the sequences were compared with the corresponding sequences in M129 registered with NCBI and the FH strain. The 70 resistant strains all showed a 2063 or 2064 site mutation in domain V of the 23S rRNA but no mutations in domain II. Site mutations of L4 or L22 can be observed in either resistant or sensitive strains, although it is not known whether this is associated with drug resistance.<\/jats:p>","DOI":"10.1155\/2014\/320801","type":"journal-article","created":{"date-parts":[[2014,1,28]],"date-time":"2014-01-28T21:01:19Z","timestamp":1390942879000},"page":"1-7","source":"Crossref","is-referenced-by-count":16,"title":["Drug Resistance Mechanisms of<i>Mycoplasma pneumoniae<\/i>to Macrolide Antibiotics"],"prefix":"10.1155","volume":"2014","author":[{"given":"Xijie","family":"Liu","sequence":"first","affiliation":[{"name":"Pediatric Apartment, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yue","family":"Jiang","sequence":"additional","affiliation":[{"name":"Beijing Institue of Tropical Medicine, Beijing Friendship Hospital, Capital Medical University, No. 95 Yongan Road, Xicheng District, Beijing 100050, 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