{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T17:56:36Z","timestamp":1790790996937,"version":"4.1.0"},"reference-count":13,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Scientific World Journal"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Enterococci are nosocomial pathogen with multiple-drug resistance by intrinsic and extrinsic mechanisms. Aminoglycosides along with cell wall inhibitors are given clinically for treating enterococcal infections. 178 enterococcal isolates were analyzed in this study.<jats:italic>E. faecalis<\/jats:italic>is identified to be the predominant<jats:italic>Enterococcus<\/jats:italic>species, along with<jats:italic>E. faecium<\/jats:italic>,<jats:italic>E. avium<\/jats:italic>,<jats:italic>E. hirae<\/jats:italic>,<jats:italic>E. durans<\/jats:italic>,<jats:italic>E. dispar<\/jats:italic>and<jats:italic>E. gallinarum<\/jats:italic>. High level aminoglycoside resistance (HLAR) by MIC for gentamicin (GM), streptomycin (SM) and both (GM + SM) antibiotics was found to be 42.7%, 29.8%, and 21.9%, respectively. Detection of aminoglycoside modifying enzyme encoding genes (AME) in enterococci was identified by multiplex PCR for<jats:italic>aac(6\u2032)-Ie-aph(2\u2032\u2032)-Ia<\/jats:italic>;<jats:italic>aph(2\u2032\u2032)-Ib<\/jats:italic>;<jats:italic>aph(2\u2032\u2032)-Ic<\/jats:italic>;<jats:italic>aph(2\u2032\u2032)-Id<\/jats:italic>and<jats:italic>aph(3\u2032)-IIIa<\/jats:italic>genes. 38.2% isolates carried<jats:italic>aac(6\u2032)-Ie-aph(2\u2032\u2032)-Ia<\/jats:italic>gene and 40.4% isolates carried<jats:italic>aph(3\u2032)-IIIa<\/jats:italic>gene.<jats:italic>aph(2\u2032\u2032)-Ib; aph(2\u2032\u2032)-Ic; aph(2\u2032\u2032)-Id<\/jats:italic>were not detected among our study isolates.<jats:italic>aac(6\u2032)-Ie-aph(2\u2032\u2032)-Ia<\/jats:italic>and<jats:italic>aph(3\u2032)-IIIa<\/jats:italic>genes were also observed in HLAR<jats:italic>E. durans<\/jats:italic>,<jats:italic>E. avium<\/jats:italic>,<jats:italic>E. hirae<\/jats:italic>, and<jats:italic>E. gallinarum<\/jats:italic>isolates. This indicates that high level aminoglycoside resistance genes are widely disseminated among isolates of enterococci from Chennai.<\/jats:p>","DOI":"10.1155\/2014\/329157","type":"journal-article","created":{"date-parts":[[2014,2,4]],"date-time":"2014-02-04T21:01:50Z","timestamp":1391547710000},"page":"1-5","source":"Crossref","is-referenced-by-count":55,"title":["High Level Aminoglycoside Resistance and Distribution of Aminoglycoside Resistant Genes among Clinical Isolates of<i>Enterococcus<\/i>Species in Chennai, India"],"prefix":"10.1155","volume":"2014","author":[{"given":"Elango","family":"Padmasini","sequence":"first","affiliation":[{"name":"Department of Microbiology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai 600 113, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Padmaraj","sequence":"additional","affiliation":[{"name":"Department of Paediatric Nephrology, Institute of Child Health and Research Centre, Chennai 600 008, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S. Srivani","family":"Ramesh","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Chennai 600 113, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"98","reference":[{"key":"10","doi-asserted-by":"publisher","DOI":"10.4103\/1755-6783.95956"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268801005271"},{"key":"6","year":"2006"},{"issue":"1","key":"7","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1128\/JCM.33.1.24-27.1995","volume":"33","year":"1995","journal-title":"Journal of Clinical Microbiology"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.47.4.1423-1426.2003"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.7860\/JCDR\/2012\/4560.2539"},{"issue":"11","key":"8","doi-asserted-by":"crossref","first-page":"2595","DOI":"10.1128\/JCM.29.11.2595-2598.1991","volume":"29","year":"1991","journal-title":"Journal of Clinical Microbiology"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1007\/s100960050310"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.4103\/0255-0857.96687"},{"key":"1","doi-asserted-by":"publisher","DOI":"10.1086\/313949"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.3.1109-1113.2003"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.3.430-450.2003"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki347"}],"container-title":["The Scientific World Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2014\/329157.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2014\/329157.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/tswj\/2014\/329157.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,8,15]],"date-time":"2020-08-15T02:03:50Z","timestamp":1597457030000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/tswj\/2014\/329157\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":13,"alternative-id":["329157","329157"],"URL":"https:\/\/doi.org\/10.1155\/2014\/329157","relation":{},"ISSN":["2356-6140","1537-744X"],"issn-type":[{"value":"2356-6140","type":"print"},{"value":"1537-744X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}