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In the present study, a hemagglutinin (BSH,\n                    <jats:italic>B. speciosus<\/jats:italic>\n                    hemagglutinin) was isolated from its fruiting bodies and enzymatic properties were also tested. The chromatographic procedure utilized comprised anion exchange chromatography on Q-Sepharose, cation exchange chromatography on CM-Cellulose, cation exchange chromatography on SP-Sepharose, and gel filtration by FPLC on Superdex 75. The hemagglutinin was a homodimer which was estimated to be approximately 31\u2009kDa in size. The activity of BSH was stable up to 60\u00b0C, while there was a precipitous drop in activity when the temperature was elevated to 70\u00b0C. BSH retained 25% hemagglutinating activity when exposed to 100\u2009mM NaOH and 25\u2009mM HCl. The activity was potently inhibited by 1.25\u2009mM\u2009Hg\n                    <jats:sup>2+<\/jats:sup>\n                    and slightly inhibited by Fe\n                    <jats:sup>2+<\/jats:sup>\n                    , Ca\n                    <jats:sup>2+<\/jats:sup>\n                    , and Pb\n                    <jats:sup>2+<\/jats:sup>\n                    . None of the sugars tested showed inhibition towards BSH. Its hemagglutinating activity towards human erythrocytes type A, type B, and type AB was higher than type O. The hemagglutinin showed antiproliferative activity towards hepatoma Hep G2 cells and mouse lymphocytic leukemia cells (L1210)\n                    <jats:italic>in vitro<\/jats:italic>\n                    , with IC\n                    <jats:sub>50<\/jats:sub>\n                    of 4.7\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M and 7.0\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M, respectively. It also exhibited HIV-1 reverse transcriptase inhibitory activity with an IC\n                    <jats:sub>50<\/jats:sub>\n                    of 7.1\u2009\n                    <jats:italic>\u03bc<\/jats:italic>\n                    M.\n                  <\/jats:p>","DOI":"10.1155\/2014\/340467","type":"journal-article","created":{"date-parts":[[2014,5,29]],"date-time":"2014-05-29T17:04:50Z","timestamp":1401383090000},"page":"1-7","source":"Crossref","is-referenced-by-count":15,"title":["A Novel Hemagglutinin with Antiproliferative Activity against Tumor Cells from the Hallucinogenic Mushroom\n                    <i>Boletus speciosus<\/i>"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9520-3200","authenticated-orcid":true,"given":"Jian","family":"Sun","sequence":"first","affiliation":[{"name":"Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, College of Biological Sciences and Engineering, Beijing University of 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