{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T23:31:12Z","timestamp":1790465472147,"version":"4.1.0"},"reference-count":20,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003669","name":"Korea Centers for Diseases Control and Prevention","doi-asserted-by":"crossref","award":["2012E4400100"],"award-info":[{"award-number":["2012E4400100"]}],"id":[{"id":"10.13039\/501100003669","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>We analyzed the whole genome sequence and resistome of the outbreak<jats:italic>Klebsiella pneumoniae<\/jats:italic>strain MP14 and compared it with those of<jats:italic>K. pneumoniae<\/jats:italic>carbapenemase- (KPC-) producing isolates that showed high similarity in the NCBI genome database. A KPC-2-producing multidrug-resistant (MDR)<jats:italic>K. pneumoniae<\/jats:italic>clinical isolate was obtained from a patient admitted to a Korean hospital in 2011. The strain MP14 was resistant to all tested<jats:italic>\u03b2<\/jats:italic>-lactams including monobactam, amikacin, levofloxacin, and cotrimoxazole, but susceptible to tigecycline and colistin. Resistome analysis showed the presence of<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>\u03b2<\/mml:mi><\/mml:mrow><\/mml:math>-lactamase genes including<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>KPC-2<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>SHV-11<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>,<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M4\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>TEM-169<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>, and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M5\"><mml:mrow><mml:msub><mml:mrow><mml:mi>b<\/mml:mi><mml:mi>l<\/mml:mi><mml:mi>a<\/mml:mi><\/mml:mrow><mml:mrow><mml:mtext>OXA-9<\/mml:mtext><\/mml:mrow><\/mml:msub><\/mml:mrow><\/mml:math>. MP14 also possessed<jats:italic>aac(6<\/jats:italic>\u2032-<jats:italic>)Ib<\/jats:italic>,<jats:italic>aadA2,<\/jats:italic>and<jats:italic>aph(3<\/jats:italic>\u2032-<jats:italic>)Ia<\/jats:italic>as aminoglycoside resistance-encoding genes,<jats:italic>mph(A)<\/jats:italic>for macrolides,<jats:italic>oqxA<\/jats:italic>and<jats:italic>oqxB<\/jats:italic>for quinolone,<jats:italic>catA1<\/jats:italic>for phenicol,<jats:italic>sul1<\/jats:italic>for sulfonamide, and<jats:italic>dfrA12<\/jats:italic>for trimethoprim. Both SNP tree and cgMLST analysis showed the close relatedness with the KPC producers (KPNIH strains) isolated from an outbreak in the USA and colistin-resistant strains isolated in Italy. The plasmid-scaffold genes in plasmids pKpQil, pKpQil-IT, pKPN3, or pKPN-IT were identified in MP14, KPNIH, and Italian strains. The KPC-2-producing MDR<jats:italic>K. pneumoniae<\/jats:italic>ST258 stain isolated in Korea was highly clonally related with MDR<jats:italic>K. pneumoniae<\/jats:italic>strains from the USA and Italy. Global spread of KPC-producing<jats:italic>K. pneumoniae<\/jats:italic>is a worrying phenomenon.<\/jats:p>","DOI":"10.1155\/2014\/352862","type":"journal-article","created":{"date-parts":[[2014,7,3]],"date-time":"2014-07-03T17:04:56Z","timestamp":1404407096000},"page":"1-6","source":"Crossref","is-referenced-by-count":17,"title":["Clonality and Resistome Analysis of KPC-Producing<i>Klebsiella pneumoniae<\/i>Strain Isolated in Korea Using Whole Genome Sequencing"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1220-5372","authenticated-orcid":true,"given":"Yangsoon","family":"Lee","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1243-8280","authenticated-orcid":true,"given":"Bong-Soo","family":"Kim","sequence":"additional","affiliation":[{"name":"Chunlab, Inc., Seoul National University, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3385-5171","authenticated-orcid":true,"given":"Jongsik","family":"Chun","sequence":"additional","affiliation":[{"name":"Chunlab, Inc., Seoul National University, Republic of Korea"},{"name":"School of Biological Sciences, Seoul National University, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hyun","family":"Yong","sequence":"additional","affiliation":[{"name":"Department of Science, University of British Columbia, Vancouver, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeong Seon","family":"Lee","sequence":"additional","affiliation":[{"name":"Division of Antimicrobial Resistance, Korea National Institute of Health, Osong, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jung Sik","family":"Yoo","sequence":"additional","affiliation":[{"name":"Division of Antimicrobial Resistance, Korea National Institute of Health, Osong, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongeun","family":"Yong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seong Geun","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, CHA Bundang Medical Center, CHA University, Seongnam, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roshan","family":"D\u2019Souza","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenneth S.","family":"Thomson","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Center for Research in Anti-Infectives and Biotechnology, Creighton University School of Medicine, Omaha, NE, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyungwon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunsop","family":"Chong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Research Institute of Bacterial Resistance, Yonsei University College of Medicine, 50 Yonsei-ro, Seodaemun-gu, Seoul 120-752, Republic of 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