{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:12:24Z","timestamp":1783606344887,"version":"3.55.0"},"reference-count":27,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["AGL-2009-12674"],"award-info":[{"award-number":["AGL-2009-12674"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004837","name":"Ministerio de Ciencia e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["20052008"],"award-info":[{"award-number":["20052008"]}],"id":[{"id":"10.13039\/501100004837","id-type":"DOI","asserted-by":"publisher"}]},{"name":"IV Plan regional de investigaci\u00f3n cient\u00edfica e innovaci\u00f3n tecnol\u00f3gica","award":["AGL-2009-12674"],"award-info":[{"award-number":["AGL-2009-12674"]}]},{"name":"IV Plan regional de investigaci\u00f3n cient\u00edfica e innovaci\u00f3n tecnol\u00f3gica","award":["20052008"],"award-info":[{"award-number":["20052008"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Brucellosis is endemic in most parts of Egypt, where it is caused mainly by<jats:italic>Brucella melitensis<\/jats:italic>biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. Knowledge of the predominant<jats:italic>Brucella<\/jats:italic>species\/strains circulating in a region is a prerequisite of a brucellosis control strategy. For this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17<jats:italic>Brucella<\/jats:italic>spp. isolates recovered from domestic ruminants (cattle, buffalo, sheep, and goat) from four governorates during a period of five years (2002\u20132007) was carried out using microbiological tests and molecular biology techniques (PCR, MLVA-15, and sequencing). Thirteen strains were identified as<jats:italic>B. melitensis<\/jats:italic>biovar 3 while all phenotypic and genetic techniques classified the remaining isolates as<jats:italic>B. abortus<\/jats:italic>(<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>2<\/mml:mn><\/mml:mrow><\/mml:math>) and<jats:italic>B. suis<\/jats:italic>biovar 1 (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:mi>n<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>2<\/mml:mn><\/mml:mrow><\/mml:math>). MLVA-15 yielded a high discriminatory power (<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M3\"><mml:mrow><mml:mi>h<\/mml:mi><mml:mo>=<\/mml:mo><mml:mn>0.801<\/mml:mn><\/mml:mrow><\/mml:math>), indicating a high genetic diversity among the<jats:italic>B. melitensis<\/jats:italic>strains circulating among domestic ruminants in Egypt. This is the first report of the isolation of<jats:italic>B. suis<\/jats:italic>from cattle in Egypt which, coupled with the finding of<jats:italic>B. abortus<\/jats:italic>, suggests a potential role of livestock as reservoirs of several zoonotic<jats:italic>Brucella<\/jats:italic>species in the region.<\/jats:p>","DOI":"10.1155\/2014\/353876","type":"journal-article","created":{"date-parts":[[2014,1,9]],"date-time":"2014-01-09T10:26:02Z","timestamp":1389263162000},"page":"1-7","source":"Crossref","is-referenced-by-count":40,"title":["Assessment of Genetic Diversity of Zoonotic<i>Brucella<\/i>spp. Recovered from Livestock in Egypt Using Multiple Locus VNTR Analysis"],"prefix":"10.1155","volume":"2014","author":[{"given":"Ahmed M. 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