{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T19:03:10Z","timestamp":1740164590656,"version":"3.37.3"},"reference-count":19,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005961","name":"National University of Cuyo","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005961","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Case Reports in Gastrointestinal Medicine"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Leiomyomas constitute 2.5% of all resected neoplasms of the stomach. They are usually asymptomatic, but may present mucosal ulceration. Aberrant DNA methylation is a well-defined epigenetic change in human neoplasms; however, gene-acquired methylation may not necessarily be related with a malignant phenotype. In this report we analyzed in a gastric leiomyoma, the methylation status of 84\u2009CpGI in tumor suppressor and DNA repair genes. We analyzed the tumor center (TC) and tumor periphery (TP) separately. We found aberrant methylation in 2\/84\u2009CpGI in the TC portion, that is,<jats:italic>MLH1<\/jats:italic>and<jats:italic>MSH3<\/jats:italic>, and 5\/84\u2009CpGI in the TP, that is,<jats:italic>MLH1<\/jats:italic>,<jats:italic>MSH3<\/jats:italic>,<jats:italic>APC<\/jats:italic>,<jats:italic>MSH6<\/jats:italic>, and<jats:italic>MGMT<\/jats:italic>. The gene with the highest methylation percentage in the TC and TP was<jats:italic>MLH1<\/jats:italic>. Given that<jats:italic>MLH1<\/jats:italic>methylation has been associated with microsatellite instability, we analyzed the status of the microsatellite Bat-26. We found that neither the TC nor the TP presented instability. The methylation of<jats:italic>MLH1<\/jats:italic>,<jats:italic>MGMT<\/jats:italic>, and<jats:italic>APC<\/jats:italic>has been described in GISTs, but to the best of our knowledge this is the first time that the methylation of these genes has been associated with gastric leiomyoma. Further research should be conducted to identify reliable molecular markers that could differentiate between GISTs and gastric leiomyomas.<\/jats:p>","DOI":"10.1155\/2014\/371638","type":"journal-article","created":{"date-parts":[[2014,12,4]],"date-time":"2014-12-04T21:01:34Z","timestamp":1417726894000},"page":"1-5","source":"Crossref","is-referenced-by-count":1,"title":["Epigenetic Alterations in a Gastric Leiomyoma"],"prefix":"10.1155","volume":"2014","author":[{"given":"M. T.","family":"Branham","sequence":"first","affiliation":[{"name":"Institute of Histology and Embryology (IHEM-CCT-CONICET) and School of Medical Sciences, National University of Cuyo, M5502JMA Mendoza, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Pellicer","sequence":"additional","affiliation":[{"name":"Pathology Laboratory, J5402EQC San Juan, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Campoy","sequence":"additional","affiliation":[{"name":"Institute of Histology and Embryology (IHEM-CCT-CONICET) and School of Medical Sciences, National University of Cuyo, M5502JMA Mendoza, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Palma","sequence":"additional","affiliation":[{"name":"Surgery Unit, San Martin Hospital, E3100 Entre R\u00edos, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Correa","sequence":"additional","affiliation":[{"name":"Surgery Unit, Espa\u00f1ol Hospital, M5501 Mendoza, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Roqu\u00e9","sequence":"additional","affiliation":[{"name":"Institute of Histology and Embryology (IHEM-CCT-CONICET) and School of Medical Sciences, National University of Cuyo, M5502JMA Mendoza, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"issue":"1","key":"1","first-page":"77","volume":"8","year":"2004","journal-title":"JSLS"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1097\/00000658-199001000-00011"},{"issue":"11","key":"3","first-page":"1758","volume":"25","year":"2004","journal-title":"Saudi Medical Journal"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880210"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra072067"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1016\/j.gassur.2003.08.002"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2011.241034"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2007.00682.x"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1038\/oncsis.2012.17"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1007\/s13402-013-0148-2"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0059064"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2006.08.2107"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2010.12.004"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgm228"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/260549"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63663-2"},{"key":"17","doi-asserted-by":"publisher","DOI":"10.1186\/1752-1947-5-516"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202178"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206365"}],"container-title":["Case Reports in Gastrointestinal Medicine"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/crigm\/2014\/371638.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/crigm\/2014\/371638.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/crigm\/2014\/371638.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2020,5,12]],"date-time":"2020-05-12T14:21:31Z","timestamp":1589293291000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/crigm\/2014\/371638\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":19,"alternative-id":["371638","371638"],"URL":"https:\/\/doi.org\/10.1155\/2014\/371638","relation":{},"ISSN":["2090-6528","2090-6536"],"issn-type":[{"type":"print","value":"2090-6528"},{"type":"electronic","value":"2090-6536"}],"subject":[],"published":{"date-parts":[[2014]]}}}