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Typhi) is a human-specific pathogen that causes typhoid fever. In this study, we constructed<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\"><mml:mrow><mml:mi>\u0394<\/mml:mi><\/mml:mrow><\/mml:math><jats:italic>ygaE<\/jats:italic>mutant and a microarray was performed to investigate the role of<jats:italic>ygaE<\/jats:italic>in regulation of gene expression changes in response to hyperosmotic stress in<jats:italic>S<\/jats:italic>. Typhi. qRT-PCR was performed to validate the microarray results. Our data indicated that<jats:italic>ygaE<\/jats:italic>was the repressor of<jats:italic>gab<\/jats:italic>operon in<jats:italic>S<\/jats:italic>. Typhi as in<jats:italic>Escherichia coli<\/jats:italic>(<jats:italic>E. coli<\/jats:italic>), though the sequence of<jats:italic>ygaE<\/jats:italic>is totally different from<jats:italic>gabC<\/jats:italic>(formerly<jats:italic>ygaE<\/jats:italic>) in<jats:italic>E. coli<\/jats:italic>. OmpF, OmpC, and OmpA are the most abundant out membrane proteins in<jats:italic>S<\/jats:italic>. Typhi. Here we report that YgaE is a repressor of both OmpF and OmpC at the early stage of hyperosmotic stress. Two-dimensional electrophoresis was applied to analyze proteomics of total proteins in wild-type strain and<mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M2\"><mml:mrow><mml:mi>\u0394<\/mml:mi><\/mml:mrow><\/mml:math><jats:italic>ygaE<\/jats:italic>strain and we found that YgaE represses the expression of OmpA at the late stage of hyperosmotic stress. Altogether, our results implied that YgaE regulates out membrane proteins in a time-dependent manner under hyperosmotic stress in<jats:italic>S<\/jats:italic>. Typhi.<\/jats:p>","DOI":"10.1155\/2014\/374276","type":"journal-article","created":{"date-parts":[[2014,1,23]],"date-time":"2014-01-23T22:39:33Z","timestamp":1390516773000},"page":"1-9","source":"Crossref","is-referenced-by-count":0,"title":["YgaE Regulates Out Membrane Proteins in<i>Salmonella enterica<\/i>Serovar Typhi under Hyperosmotic Stress"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7717-8259","authenticated-orcid":true,"given":"Min","family":"Wang","sequence":"first","affiliation":[{"name":"Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Feng","sequence":"additional","affiliation":[{"name":"Clinical Laboratory, The Second Affiliated Hospital of Soochow University, Suzhou 215004, 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