{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T03:10:39Z","timestamp":1791083439766,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81270434"],"award-info":[{"award-number":["81270434"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Oxidative Medicine and Cellular Longevity"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Nitrates are the most frequently prescribed and utilized drugs worldwide. The elderly are a major population receiving nitrate therapy. Both nitrates and aging can increase in vivo reactive oxygen species (ROS) and reactive nitrogen species (RNS). To date, the effects of aging upon nitrate-induced ROS\/RNS alteration are unknown. The present study tested the effects of aging upon nitrate-induced ROS\/RNS alteration in vivo. 32 adults and 43 elderly unstable angina (UA) patients were subjected to 48 hours of isosorbide dinitrate intravenous injection (50\u2009<jats:italic>\u03bc<\/jats:italic>g\/minutes) in this clinical study. Blood samples were obtained at baseline and conclusion. Outcome measures of oxidative stress included plasma malondialdehyde (MDA), myeloperoxidase (MPO), and reduced glutathione (GSH). Plasma concentrations of NO<jats:italic>x<\/jats:italic>and nitrotyrosine served as markers of RNS. Because of the significant differences in basic clinical characters between adults and the elderly, we designed an additional experiment determining ROS\/RNS stress in rat cardiac tissue. Additionally, rat thoracic aortic NOS activity served as a marker indicating endothelial function. Our study demonstrated that nitrate therapy significantly increased in vivo ROS\/RNS stress in the elderly compared to adult patients, confirmed by animal data. Decreased NOS activity was observed in old rats. Taken together, the present study\u2019s data suggests a synergism between nitrate treatment and the aging process.<\/jats:p>","DOI":"10.1155\/2014\/376515","type":"journal-article","created":{"date-parts":[[2014,3,23]],"date-time":"2014-03-23T17:01:28Z","timestamp":1395594088000},"page":"1-7","source":"Crossref","is-referenced-by-count":26,"title":["Aging Aggravates Nitrate-Mediated ROS\/RNS Changes"],"prefix":"10.1155","volume":"2014","author":[{"given":"Qian","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lifen","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Neurology, The Second Affiliated Hospital of Chong Qing Medical University, Chong Qing, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shujuan","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayne Bond","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Emergency Medicine, Thomas Jefferson University, Philadelphia, PA 19107, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Le Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Heart Center, Beijing Chaoyang Hospital-Affiliate of Beijing Capital Medical University, 8 Gongtinan Road, Beijing 100020, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing Hua","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Cardiology, Beijing An Zhen Hospital, Capital Medical University, Beijing Institute of Heart, Lung and Blood Vessel Disease, Beijing 100029, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.107.187998"},{"key":"2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/480895"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1007\/s10522-009-9253-z"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1354.003"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-012-9421-y"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-011-9259-8"},{"issue":"1","key":"7","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1172\/JCI117637","volume":"95","year":"1995","journal-title":"The Journal of Clinical Investigation"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-005-1455-5"},{"issue":"23","key":"9","doi-asserted-by":"crossref","first-page":"2810","DOI":"10.1161\/01.CIR.102.23.2810","volume":"102","year":"2000","journal-title":"Circulation"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1023\/A:1020766502316"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1042\/CS20100369"},{"issue":"1","key":"12","first-page":"11","volume":"16","year":"2009","journal-title":"Cardiology Journal"},{"key":"13","doi-asserted-by":"publisher","DOI":"10.1097\/FJC.0b013e3181d265a3"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(98)00456-2"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1016\/S0735-1097(01)01510-8"}],"container-title":["Oxidative Medicine and Cellular Longevity"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2014\/376515.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2014\/376515.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/omcl\/2014\/376515.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T06:12:38Z","timestamp":1498111958000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.hindawi.com\/journals\/omcl\/2014\/376515\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":15,"alternative-id":["376515","376515"],"URL":"https:\/\/doi.org\/10.1155\/2014\/376515","relation":{},"ISSN":["1942-0900","1942-0994"],"issn-type":[{"value":"1942-0900","type":"print"},{"value":"1942-0994","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}