{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T17:02:00Z","timestamp":1740157320567,"version":"3.37.3"},"reference-count":22,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Veterinary Medicine International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>Dogs are common hosts to the fungal organism<jats:italic>Blastomyces dermatitidis<\/jats:italic>, which causes the systemic disease blastomycosis. The goal of our study was to compare the reactivity of two<jats:italic>B. dermatitidis<\/jats:italic>yeast lysate antigens prepared from dog isolates (ERC-2, Wisconsin; T-58, Tennessee) and two lysate antigens prepared from human isolates (B5931 and B5896, Minnesota) against 48 serum specimens from dogs with confirmed blastomycosis using the indirect enzyme-linked immunosorbent assay (ELISA). Secondarily, we used three different ELISA substrates (Ultra TMB: A, SureBlue: B, and SureBlue Reserve: C) to compare the effectiveness of each substrate. Mean absorbance values ranged from 0.446 (B) to 0.651 (C) for the B5931 antigen and from 0.393 (B) to 0.540 (C) for the ERC-2 antigen in Trial 1. In Trial 2, the absorbance values ranged from 0.628 (B) to 0.909 (A) for the B5896 antigen and from 0.828 (B) to 1.375 (C) for the T-58 antigen. In Trial 1, the lysate antigen prepared from the human isolate B5931 exhibited the highest absorbance value and in Trial 2 the lysate prepared from the dog isolate T-58 was the most reactive. The overall results thus indicated that the T-58 lysate was the optimal reagent when used to detect antibody with the Sure-Blue Reserve substrate. Our laboratory is continuing to study<jats:italic>B. dermatitidis<\/jats:italic>antigen and substrate combinations for the reliable immunodiagnosis of blastomycosis in humans and animals.<\/jats:p>","DOI":"10.1155\/2014\/376725","type":"journal-article","created":{"date-parts":[[2014,3,3]],"date-time":"2014-03-03T07:56:44Z","timestamp":1393833404000},"page":"1-4","source":"Crossref","is-referenced-by-count":1,"title":["<i>Blastomyces dermatitidis<\/i>: Antibody Detection in Sera from Dogs with Blastomycosis with Yeast Lysate Antigens Produced from Human and Dog Isolates"],"prefix":"10.4061","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6487-0257","authenticated-orcid":true,"given":"Katie","family":"Mondada","sequence":"first","affiliation":[{"name":"Department of Biological Sciences, Idaho State University, P.O. Box 8007, 921 S. 8th Avenue, Pocatello, ID 83209, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9046-8763","authenticated-orcid":true,"given":"Jessie","family":"Fullmer","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Idaho State University, P.O. Box 8007, 921 S. 8th Avenue, Pocatello, ID 83209, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0452-6523","authenticated-orcid":true,"given":"Eric","family":"Hungerford","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Idaho State University, P.O. Box 8007, 921 S. 8th Avenue, Pocatello, ID 83209, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katrina","family":"Novack","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Idaho State University, P.O. Box 8007, 921 S. 8th Avenue, Pocatello, ID 83209, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristen","family":"Vickers","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Idaho State University, P.O. Box 8007, 921 S. 8th Avenue, Pocatello, ID 83209, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9862-1184","authenticated-orcid":true,"given":"Gene","family":"Scalarone","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, Idaho State University, P.O. 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