{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T22:55:45Z","timestamp":1790290545083,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p>\n                    Melioidosis is a severe infectious disease caused by\n                    <jats:italic>Burkholderia pseudomallei<\/jats:italic>\n                    . It is refractory to antibiotic treatment and there is currently no licensed vaccine. In this report we detail the construction and protective efficacy of a polysaccharide-protein conjugate composed of\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    lipopolysaccharide and the\n                    <mml:math xmlns:mml=\"http:\/\/www.w3.org\/1998\/Math\/MathML\" id=\"M1\">\n                      <mml:mrow>\n                        <mml:msub>\n                          <mml:mrow>\n                            <mml:mtext>H<\/mml:mtext>\n                          <\/mml:mrow>\n                          <mml:mrow>\n                            <mml:mtext>c<\/mml:mtext>\n                          <\/mml:mrow>\n                        <\/mml:msub>\n                      <\/mml:mrow>\n                    <\/mml:math>\n                    fragment of tetanus toxin. Immunisation of mice with the lipopolysaccharide-conjugate led to significantly reduced bacterial burdens in the spleen 48 hours after challenge and afforded significant protection against a lethal challenge with\n                    <jats:italic>B. pseudomallei<\/jats:italic>\n                    . The conjugate generated significantly higher levels of antigen-specific IgG1 and IgG2a than in lipopolysaccharide-immunised mice. Immunisation with the conjugate also demonstrated a bias towards Th1 type responses, evidenced by high levels of IgG2a. In contrast, immunisation with unconjugated lipopolysaccharide evoked almost no IgG2a demonstrating a bias towards Th2 type responses. This study demonstrates the effectiveness of this approach in the development of an efficacious and protective vaccine against melioidosis.\n                  <\/jats:p>","DOI":"10.1155\/2014\/392170","type":"journal-article","created":{"date-parts":[[2014,5,7]],"date-time":"2014-05-07T17:12:18Z","timestamp":1399482738000},"page":"1-10","source":"Crossref","is-referenced-by-count":25,"title":["Protection against Experimental Melioidosis following Immunisation with a Lipopolysaccharide-Protein Conjugate"],"prefix":"10.1155","volume":"2014","author":[{"given":"Andrew E.","family":"Scott","sequence":"first","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah A.","family":"Ngugi","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas R.","family":"Laws","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Corser","sequence":"additional","affiliation":[{"name":"Fleet Bioprocessing Ltd., Pale Lane Farm, Hartley Wintney RG27 8DH, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claire L.","family":"Lonsdale","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Riccardo V.","family":"D\u2019Elia","sequence":"additional","affiliation":[{"name":"Defence Science and Technology Laboratory, Porton Down, Salisbury SP4 0JQ, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard W.","family":"Titball","sequence":"additional","affiliation":[{"name":"College of Life and Environmental Sciences, University of Exeter, Exeter EX4 4QD, UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"E. 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