{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,4]],"date-time":"2026-06-04T10:53:11Z","timestamp":1780570391009,"version":"3.54.1"},"reference-count":43,"publisher":"Hindawi Limited","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"name":"Chinese National Key Project of Infectious Disease","award":["2012ZX10003008-004"],"award-info":[{"award-number":["2012ZX10003008-004"]}]},{"name":"Chinese National Key Project of Infectious Disease","award":["20110181110046"],"award-info":[{"award-number":["20110181110046"]}]},{"name":"Fund of Doctoral Scientific Research of MOE","award":["2012ZX10003008-004"],"award-info":[{"award-number":["2012ZX10003008-004"]}]},{"name":"Fund of Doctoral Scientific Research of MOE","award":["20110181110046"],"award-info":[{"award-number":["20110181110046"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Journal of Immunology Research"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Mycobacterium tuberculosis<\/jats:italic>infection is still a major global public health problem. Presently the only tuberculosis (TB) vaccine available is Bacille Calmette-Gu\u00e9rin (BCG), although it fails to adequately protect against pulmonary TB in adults. To solve this problem, the development of a new effective vaccine is urgently desired. BCG-prime DNA-booster vaccinations strategy has been shown to induce greater protection against tuberculosis (TB) than BCG alone. Some studies have demonstrated that the two genes (Rv1769 and Rv1772) are excellent T-cell antigens and could induce T-cell immune responses. In this research, we built BCG-C or BCG-P prime-recombination plasmid PcDNA3.1-Rv1769 or PcDNA3.1-Rv1772 boost vaccinations strategy to immunize BALB\/c mice and evaluated its immunogenicity. The data suggests that the BCG-C+3.1-72 strategy could elicit the most long-lasting and strongest Th1-type cellular immune responses and the BCG-C+3.1-69 strategy could induce the high level CD8+ T-cell response at certain time points. These findings support the ideas that the prime-boost strategy as a combination of vaccines may be better than a single vaccine for protection against tuberculosis.<\/jats:p>","DOI":"10.1155\/2014\/395626","type":"journal-article","created":{"date-parts":[[2014,3,11]],"date-time":"2014-03-11T21:01:42Z","timestamp":1394571702000},"page":"1-8","source":"Crossref","is-referenced-by-count":7,"title":["A<i>Mycobacterium bovis<\/i>BCG-Naked DNA Prime-Boost Vaccination Strategy Induced CD4<sup>+<\/sup>and CD8<sup>+<\/sup>T-Cell Response against<i>Mycobacterium tuberculosis<\/i>Immunogens"],"prefix":"10.1155","volume":"2014","author":[{"given":"Miao","family":"Lu","sequence":"first","affiliation":[{"name":"Laboratory of Infection and Immunity, School of Basic Medical Science, West China Center of Medical Sciences, Sichuan University, No. 17, Third Section, Ren Min Nan Road, Chengdu, Sichuan 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi Yang","family":"Xia","sequence":"additional","affiliation":[{"name":"Laboratory of Infection and Immunity, School of Basic Medical Science, West China Center of Medical Sciences, Sichuan University, No. 17, Third Section, Ren Min Nan Road, Chengdu, Sichuan 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lang","family":"Bao","sequence":"additional","affiliation":[{"name":"Laboratory of Infection and Immunity, School of Basic Medical Science, West China Center of Medical Sciences, Sichuan University, No. 17, Third Section, Ren Min Nan Road, Chengdu, Sichuan 610041, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"98","reference":[{"issue":"1","key":"2","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1093\/clinids\/20.1.11","volume":"20","year":"1995","journal-title":"Clinical Infectious 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