{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,10]],"date-time":"2026-04-10T22:00:24Z","timestamp":1775858424673,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003803","name":"University of Hong Kong","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003803","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Areas of Excellence Scheme - University Grants Committee"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Background.<\/jats:italic>The deregulated tumorigenic long non-coding RNA (lncRNA) has been reported in several malignancies. However, there is still no comprehensive study on tongue squamous cell carcinoma (SCC).<jats:italic>Methods.<\/jats:italic>Functional reannotation for the human lncRNA was carried out by ncFANs. Real-time quantitative PCR was used to validate the identified lncRNAs.<jats:italic>Results.<\/jats:italic>Using the functional annotation algorithm from ncFANs, 8 differentially expressed lncRNAs were identified. Lnc-PPP2R4-5, lnc-SPRR2D-1, lnc-MAN1A2-1, lnc-FAM46A-1, lnc-MBL2-4:1, and lnc-MBL2-4:3 were upregulated in the microdissected tongue SCC tissues. In comparison, lnc-AL355149.1-1 and lnc-STXBP5-1 showed significant downregulation. High level of lnc-MBL2-4:3 was significantly associated with the node positive tongue SCC patients. Further, patients with advanced T-stage demonstrated a further reduction of lnc-AL355149.1-1 in the tumor tissues. Treatment of tongue SCC cells with 5-fluorouracil and paclitaxel can reserve the expression patterns observed in the tongue SCC tissues. Further, changes of lnc-MBL2-4:3 and lnc-AL355149.1-1 expression levels were noticed in the cisplatin-resistant tongue SCC cells.<jats:italic>Conclusions.<\/jats:italic>Our results demonstrated that functional reannotation allows us to identify novel lncRNAs using the existing gene expression array dataset. The association of lncRNA with the T-stage and nodal status of tongue SCC patients suggested that lncRNA deregulation was involved in the pathogenesis of tongue SCC.<\/jats:p>","DOI":"10.1155\/2014\/405860","type":"journal-article","created":{"date-parts":[[2014,6,22]],"date-time":"2014-06-22T17:02:00Z","timestamp":1403456520000},"page":"1-10","source":"Crossref","is-referenced-by-count":30,"title":["Long Non-Coding RNA Deregulation in Tongue Squamous Cell Carcinoma"],"prefix":"10.1155","volume":"2014","author":[{"given":"Wei","family":"Gao","sequence":"first","affiliation":[{"name":"Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Road, Kowloon, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jimmy Yu-Wai","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Road, Kowloon, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thian-Sze","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Surgery, LKS Faculty of Medicine, The University of Hong Kong, Queen Mary Hospital, 102 Pokfulam Road, Kowloon, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20138"},{"issue":"2","key":"2","first-page":"571","volume":"32","year":"2012","journal-title":"Anticancer Research"},{"key":"3","doi-asserted-by":"publisher","DOI":"10.1002\/hed.23486"},{"key":"4","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(02)00052-0"},{"key":"5","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2006.11.015"},{"key":"6","doi-asserted-by":"publisher","DOI":"10.1101\/sqb.2010.75.060"},{"key":"7","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/943539"},{"key":"8","doi-asserted-by":"publisher","DOI":"10.1089\/cbr.2013.1574"},{"key":"9","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065309"},{"key":"10","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkr432"},{"key":"11","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2011.07.008"},{"key":"12","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2013.09.007"},{"key":"13","year":"2013","journal-title":"International Journal of Cancer"},{"key":"14","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2012.1254"},{"key":"15","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2010.07126.x"},{"key":"16","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2014.02.144"},{"issue":"1","key":"17","doi-asserted-by":"crossref","first-page":"358","DOI":"10.3892\/or.2013.2850","volume":"31","year":"2014","journal-title":"Oncology Reports"},{"key":"18","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-5-20"},{"key":"19","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.24.720"},{"issue":"7","key":"20","doi-asserted-by":"crossref","first-page":"1750","DOI":"10.1111\/febs.12737","volume":"281","year":"2014","journal-title":"FEBS Journal"},{"issue":"10","key":"21","volume":"8","year":"2013","journal-title":"PLoS ONE"},{"key":"22","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0065309"}],"container-title":["BioMed Research International"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/405860.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/405860.xml","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/downloads.hindawi.com\/journals\/bmri\/2014\/405860.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2019,8,11]],"date-time":"2019-08-11T16:22:01Z","timestamp":1565540521000},"score":1,"resource":{"primary":{"URL":"http:\/\/www.hindawi.com\/journals\/bmri\/2014\/405860\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014]]},"references-count":22,"alternative-id":["405860","405860"],"URL":"https:\/\/doi.org\/10.1155\/2014\/405860","relation":{},"ISSN":["2314-6133","2314-6141"],"issn-type":[{"value":"2314-6133","type":"print"},{"value":"2314-6141","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014]]}}}