{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T03:55:39Z","timestamp":1787630139114,"version":"build-2736575974"},"reference-count":37,"publisher":"Wiley","license":[{"start":{"date-parts":[[2014,1,1]],"date-time":"2014-01-01T00:00:00Z","timestamp":1388534400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"funder":[{"DOI":"10.13039\/501100003172","name":"Henan University of Science and Technology","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003172","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["BioMed Research International"],"published-print":{"date-parts":[[2014]]},"abstract":"<jats:p><jats:italic>Objectives<\/jats:italic>. Temporomandibular joint (TMJ) disorders are common disease in maxillofacial surgery. The aim of this study is to regenerate fibrocartilage with a mixture of TMJ fibrochondrocytes and periodontal ligament derived mesenchymal stem cells (PD-MSCs).<jats:italic>Materials and Methods<\/jats:italic>. Fibrochondrocytes and PD-MSC were cocultured (ratio 1\u2009:\u20091) for 3 weeks. Histology and glycosaminoglycans (GAGs) assay were performed to examine the deposition of GAG. Green florescent protein (GFP) was used to track PD-MSC. Conditioned medium of PD-MSCs was collected to study the soluble factors. Gene expression of fibrochondrocytes cultured in conditioned medium was tested by quantitative PCR (qPCR).<jats:italic>Results<\/jats:italic>. Increased proliferation of TMJ-CH was observed in coculture pellets when compared to monoculture. Enhanced GAG production in cocultures was shown by histology and GAG quantification. Tracing of GFP revealed the fact that PD-MSC disappears after coculture with TMJ-CH for 3 weeks. In addition, conditioned medium of PD-MSC was also shown to increase the proliferation and GAG deposition of TMJ-CH. Meanwhile, results of qPCR demonstrated that conditioned medium enhanced the expression levels of matrix-related genes in TMJ-CH.<jats:italic>Conclusions<\/jats:italic>. Results from this study support the mechanism of MSC-chondrocyte interaction, in which MSCs act as secretor of soluble factors that stimulate proliferation and extracellular matrix deposition of chondrocytes.<\/jats:p>","DOI":"10.1155\/2014\/410167","type":"journal-article","created":{"date-parts":[[2014,11,10]],"date-time":"2014-11-10T16:01:58Z","timestamp":1415635318000},"page":"1-8","source":"Crossref","is-referenced-by-count":9,"title":["Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes"],"prefix":"10.1155","volume":"2014","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2948-8233","authenticated-orcid":true,"given":"Jianli","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Stomatology, The First Affiliated Hospital of Henan, University of Science and Technology, 24 Jinghua Road, Jianxi District, Luoyang, Henan 471003, 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